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Development of a photodiode array biochip using a bipolar semiconductor and its application to detection of human papilloma virus

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Abstract

We describe a DNA microarray system using a bipolar integrated circuit photodiode array (PDA) chip as a new platform for DNA analysis. The PDA chip comprises an 8 × 6 array of photodiodes each with a diameter of 600 μm. Each photodiode element acts both as a support for an immobilizing probe DNA and as a two-dimensional photodetector. The usefulness of the PDA microarray platform is demonstrated by the detection of high-risk subtypes of human papilloma virus (HPV). The polymerase chain reaction (PCR)-amplified biotinylated HPV target DNA was hybridized with the immobilized probe DNA on the photodiode surface, and the chip was incubated in an anti-biotin antibody-conjugated gold nanoparticle solution. The silver enhancement by the gold nanoparticles bound to the biotin of the HPV target DNA precipitates silver metal particles at the chip surfaces, which block light irradiated from above. The resulting drop in output voltage depends on the amount of target DNA present in the sample solution, which allows the specific detection and the quantitative analysis of the complementary target DNA. The PDA chip showed high relative signal ratios of HPV probe DNA hybridized with complementary target DNA, indicating an excellent capability in discriminating HPV subtypes. The detection limit for the HPV target DNA analysis improved from 1.2 nM to 30 pM by changing the silver development time from 5 to 10 min. Moreover, the enhanced silver development promoted by the gold nanoparticles could be applied to a broader range of target DNA concentration by controlling the silver development time.

An optical image of the PDA chip and target DNA detection through silver enhancement

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Acknowledgment

This research was partially supported by the Korea Industrial Technology Foundation and Korean Ministry of Commerce, Industry and Energy under contract 10024715-2006-11. We thank Dr. Jong Won Kim of Biomedlab Co. for his helpful discussions.

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Authors and Affiliations

  1. Department of Applied Chemistry, Hanyang University, Sa-1 dong 1271, Ansan, 425-791, South Korea

    Taek Jin Baek, Pan Yun Park, Kwi Nam Han & Gi Hun Seong

  2. 8F-8, Hightech Center, Gyeonggi Technopark, Celltek, Co., Ltd., Ansan, 426-170, South Korea

    Ho Taik Kwon

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  1. Taek Jin Baek
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  2. Pan Yun Park
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  3. Kwi Nam Han
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  4. Ho Taik Kwon
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Correspondence to Gi Hun Seong.

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Baek, T.J., Park, P.Y., Han, K.N. et al. Development of a photodiode array biochip using a bipolar semiconductor and its application to detection of human papilloma virus. Anal Bioanal Chem 390, 1373–1378 (2008). https://doi.org/10.1007/s00216-007-1814-x

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  • DOI: https://doi.org/10.1007/s00216-007-1814-x

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