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Characterization of anticonvulsant and antiepileptogenic potential of thymol in various experimental models

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Abstract

The present study was to investigate the anticonvulsant and antiepileptogenic potential of thymol. Anticonvulsant activity of thymol (5–100 mg/kg i.p.) was studied using maximal electroshock, pentylenetetrazole (PTZ), strychnine and 4-aminopyridine (4-AP) models. Thymol at the selected dose was also studied for its effect on locomotion. Antiepileptogenic property of thymol (5–25 mg/kg) was evaluated using PTZ-induced kindling model along with its effect on malondialdehyde and glutathione levels. Thymol (50 and 100 mg/kg, i.p.) showed anticonvulsant activity against maximal electroshock and pentylenetetrazole (66.66 and 83.33 % protection at 50 and 100 mg/kg, respectively) model but not against strychnine and 4-aminopyridine models. Thymol exhibited decreased locomotor activity in dose-dependent manner at the same dose range. Thymol at the dose of (25 mg/kg, i.p.) significantly decreased the seizure score, increased glutathione levels and decreased malondialdehyde levels in pentylenetetrazole-induced kindling model. Thymol exhibited significant anticonvulsant and antiepileptogenic property.

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Abbreviations

GABAA :

Gamma aminobutyric acid

HLE:

Hind limb extension

ICT:

Institute of Chemical Technology

IAEC:

Institutional Animals Ethics Committee

MES:

Maximal electroshock

MDA:

Malondialdehyde

PTZ:

Pentylenetetrazole

ROS:

Reactive oxygen species

STR:

Strychnine

4-AP:

4-Aminopyridine

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Acknowledgement

The authors would like to thank to UGC-SAP for funding the research.

Conflict of interest

The authors have declared that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, 400019, Maharashtra, India

    Jayant Sancheti, Mohd Farooq Shaikh, Rahul Chaudhari, Gauresh Somani, Sachin Patil, Pankaj Jain & Sadhana Sathaye

  2. Brain Research Institute, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Petaling Jaya, Selangor, 46150, Malaysia

    Mohd Farooq Shaikh

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  1. Jayant Sancheti
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  2. Mohd Farooq Shaikh
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  3. Rahul Chaudhari
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  4. Gauresh Somani
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  5. Sachin Patil
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  6. Pankaj Jain
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  7. Sadhana Sathaye
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Correspondence to Sadhana Sathaye.

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Sancheti, J., Shaikh, M.F., Chaudhari, R. et al. Characterization of anticonvulsant and antiepileptogenic potential of thymol in various experimental models. Naunyn-Schmiedeberg's Arch Pharmacol 387, 59–66 (2014). https://doi.org/10.1007/s00210-013-0917-5

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  • DOI: https://doi.org/10.1007/s00210-013-0917-5

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