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Responses of Microbial Community to Di-(2-ethylhcxyl) Phthalate Contamination in Brown Soil

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A Correction to this article was published on 19 June 2020

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Abstract

Di-(2-ethylhcxyl) phthalate (DEHP) is applied as plasticizer, which results in the pollution of environment. In this study, the effects of DEHP on soil microbial functions, structure and genetic diversity were investigated. The concentration of DEHP in the soil were 0, 0.1, 1, 10 and 50 mg/kg, and the experimental period were 28 days. DEHP reduced the quantity, abundance, species dominance and homogeneity of soil microbes during the first 14 days. In addition, microbial utilization efficiency of carbon (carbohydrates, aliphatics, amino acids, metabolites) was impacted after 28 days, though the effects gradually weakened. Based on denaturing gradient gel electrophoresis and clone library analysis, in the presence of DEHP, the dominant microbes in the DEHP-contaminated soil were Sphingomonas and Bacillus, which belonged to the Acidobacteria and Proteobacteriav, respectively. With 0.1 or 1 mg/kg of DEHP, the relative abundances of Acidobacteria were higher, and with 10 or 50 mg/kg of DEHP, the relative abundances of Proteobacteria were higher.

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Change history

  • 19 June 2020

    In the original publication of the article, there was an error in the abstract section. The incorrect sentence "the dominant microbes in the DEHP-contaminated soil were Sphingomonas and Bacillus, which belonged to the Acidobacteria and Proteobacteriav, respectively" should be revised to ���the dominant microbes in the DEHP-contaminated soil were belonged to the Acidobacteria and Proteobacteria, respectively.��� This has been corrected with this erratum.

References

  • Averill C, Hawkes CV (2016) Ectomycorrhizal fungi slow soil carbon cycling. Ecol Lett 19:937–947

    Article  Google Scholar 

  • Azarbad H, Niklińska M, Laskowski R et al (2015) Microbial community composition and functions are resilient to metal pollution along two forest soil gradients. FEMS Microbiol Ecol 91:1–11

    Article  CAS  Google Scholar 

  • Becerra-Castro C, Lopes AR, Vaz-Moreira I et al (2015) Wastewater reuse in irrigation: a microbiological perspective on implications in soil fertility and human and environmental health. Environ Int 75:117–135

    Article  CAS  Google Scholar 

  • Chen HL, Zhuang RS, Yao J et al (2013) A comparative study on the impact of phthalate esters on soil microbial activity. Bull Environ Contam Toxicol 91:217–223

    Article  CAS  Google Scholar 

  • Deng X, Li QF, Ni CY et al (2007) Effect of dimethoate on microbial populations in soil. Ecol Environ 16:416–420 (in Chinese)

    Google Scholar 

  • Feng NX, Yu J, Mo CH et al (2018) Biodegradation of di-n-butyl phthalate (DBP) by a novel endophytic Bacillus megaterium strain YJB3. Sci Total Environ 616–617:117–127

    Article  CAS  Google Scholar 

  • Frąc M, Oszust K, Lipiec J (2012) Community level physiological profiles (CLPP), characterization and microbial activity of soil amended with dairy sewage sludge. Sensors 12:3253–3268

    Article  Google Scholar 

  • Gao J, Shi YY, Zhou HF et al (2016) Application of modified attapulgite in phthalate acid ester-contaminated soil: effects on phthalate acid ester dissipation and the composition of soil microbial community. Environ Sci Pollut Res 23:15175–15182

    Article  CAS  Google Scholar 

  • Gao JP, Song PP, Wang G, Ying et al (2018) Responses of atrazine degradation and native bacterial community in soil to Arthrobacter sp. strain HB-5. Ecotoxicol Environ Saf 159:317–323

    Article  CAS  Google Scholar 

  • Gianfreda L, Rao MA (2004) Potential of extra cellular enzymes in remediation of polluted soils: a review. Enzyme Microbial Technol 35:339–354

    Article  CAS  Google Scholar 

  • Gryta A, Frąc M, Oszust K (2014) The application of the biolog EcoPlate approach in ecotoxicological evaluation of dairy sewage sludge. Appl Biochem Biotechnol 174:1434–1443

    Article  CAS  Google Scholar 

  • Guo PP, Zhu LS, Wang J et al (2014) Effects of low concentration endosulfan on some enzymatic activities and bacterial community structure in brunisolic soil. J Agro-Environ Sci 33:2149–2154

    CAS  Google Scholar 

  • Guo PP, Zhu LS, Wang JH et al (2015) Effects of alkyl-imidazolium ionic liquid [Omim]Cl on the functional diversity of soil microbial communities. Environ Sci Pollut Res 22:1–8

    Article  CAS  Google Scholar 

  • He LZ, Gielen G, Bolan NS et al (2015) Contamination and remediation of phthalic acid esters in agricultural soils in China: a review. Agron Sustain Dev 35:519–534

    Article  CAS  Google Scholar 

  • Hu X, Wu N, Yin P, Wu Y (2013) Effects of snowpack and litter input on soil microbial count and biomass in the Eastern Tibetan Plateau. Ecol Sci 32:359–364 (in Chinese)

    Google Scholar 

  • Kim D, Cui R, Moon J et al (2019) Soil ecotoxicity study of DEHP with respect to multiple soil species. Chemosphere 216:387–395

    Article  CAS  Google Scholar 

  • Ma TT, Zhou W, Chen LK et al (2017) Toxicity effects of di-(2-ethylhexyl) phthalate to Eisenia fetida at enzyme, cellular and genetic levels. PLoS ONE 12:e0173957

    Article  CAS  Google Scholar 

  • Nakashima R, Hayashi Y, Md K et al (2013) Exposure to DEHP decreased four fatty acid levels in plasma of prepartum mice. Toxicology 309:52–60

    Article  CAS  Google Scholar 

  • Pradeep S, Sarath Josh MK, Binod P et al (2015) Achromobacter denitrificans strain SP1 efficiently remediates di(2-ethylhexyl)phthalate. Ecotoxicol Environ Saf 112:114–121

    Article  CAS  Google Scholar 

  • Qin H, Lin X, Chen R, Yin R (2005) Effects of DEHP on dehydrogenase activity and microbial functional diversity in soil. Acta Pedol Sin 42:829–834 (in Chinese)

    CAS  Google Scholar 

  • Qiu YL, Li Y (2018) A theoretical method for the high-sensitivity fluorescence detection of PAEs through double-substitution modification. Environ Sci Pollut Res 25:34684–34692

    Article  CAS  Google Scholar 

  • Ren L, Lin Z, Liu H, Hu H (2018) Bacteria-mediated phthalic acid esters degradation and related molecular mechanisms. Appl Microbiol Biotechnol 102:1085–1096

    Article  CAS  Google Scholar 

  • Rhind SM, Kyle CE, Kerr C et al (2013) Concentrations and geographic distribution of selected organic pollutants in Scottish surface soils. Environ Pollut 182:15–27

    Article  CAS  Google Scholar 

  • Shi Y, Grogan P, Sun HB et al (2015) Multi-scale variability analysis reveals the importance of spatial distance in shaping Arctic soil microbial functional communities. Soil Biol Biochem 86:126–134

    Article  CAS  Google Scholar 

  • Song PP, Gao JP, Li XX et al (2019) Phthalate induced oxidative stress and DNA damage in earthworms (Eisenia fetida). Environ Int 129:10–17

    Article  CAS  Google Scholar 

  • Su JQ, Wei B, Xu CY et al (2014) Functional metagenomic characterization of antibiotic resistance genes in agricultural soils from China. Environ Int 65:9–15

    Article  CAS  Google Scholar 

  • Surhio MA, Talpur FN, Nizamani SM et al (2017) Effective bioremediation of endocrine-disrupting phthalate esters, mediated by bacillus strains. Water Air Soil Pollut 228:386–393

    Article  CAS  Google Scholar 

  • Taha M, Kadali KK, Al-Hothaly K et al (2015) An effective microplate method (Biolog MT2) for screening native lignocellulosic-straw-degrading bacteria. Ann Microbiol 65:2053–2064

    Article  CAS  Google Scholar 

  • Wang J, Luo YM, Ma WT et al (2013) Pollution characteristics and health risk assessment of phthalate esters in typical intensive agricultural soils. China Environ Sci 33:2235–2242 (in Chinese)

    CAS  Google Scholar 

  • Wang L, Wang LH, Chang Q et al (2017) Effects of di-(2-ethylhexyl) phthalate on microbial biomass carbon and microbial community structural diversity in a Mollisol. Eur J Soil Sci 68:897–908

    Article  CAS  Google Scholar 

  • Wang Z, Feng C, Guo W et al (2010) Effects of isoproturon on soil microbial populations and enzyme activities. Chin J Appl Environ Biol 16:688–691 (in Chinese)

    CAS  Google Scholar 

  • Wang ZG, Hu YL, Xu WH et al (2015) Impacts of dimethyl phthalate on the bacterial community and functions in black soils. Front Microbiol 6:405. https://doi.org/10.3389/fmicb.2015.00405

    Article  Google Scholar 

  • Wu ZY, Lin WX, Chen ZF et al (2013) Characteristics of soil microbial community under different vegetation types in Wuyishan National Nature Reserve, East China. Chin J Appl Ecol 24:2301–2309 (in Chinese)

    CAS  Google Scholar 

  • Wang W, Craig ZR, Basavarajappa MS et al (2012) Di (2-ethylhexyl) phthalate inhibits growth of mouse ovarian antral follicles through an oxidative stress pathway. Toxicol Appl Pharmacol 258:288–295

    Article  CAS  Google Scholar 

  • Xia QB, Wang J, Zhu LS et al (2016) Ecological effects of di (2-ethylhexyl) phthalate on soil microorganisms. J Agro-Environ Sci 35:1344–1350 (in Chinese)

    Google Scholar 

  • Xie HJ, Shi YJ, Zhang J et al (2010) Degradation of phthalate esters (PAEs) in soil and the effects of PAEs on soil microcosm activity. J Chem Technol Biotechnol 85:1108–1116

    Article  CAS  Google Scholar 

  • Xu JB, Feng YZ, Barros N et al (2017) Exploring the potential of microcalorimetry to study soil microbial metabolic diversity. J Therm Anal Calorim 127:1457–1465

    Article  CAS  Google Scholar 

  • Yan YZ, Wolkers-Rooijackers J, Nout MJR, Han BZ (2013) Microbial diversity and dynamics of microbial communities during back-slop soaking of soybeans as determined by PCR–DGGE and molecular cloning. World J Microbiol Biotechnol 29:1969–1974

    Article  Google Scholar 

  • Zhang M, Wu XW, Zhang FH (2015) Identification and biodegradation characteristics of a phthalate ester degrading bacterium. Agric Sci Technol 16:1363–1366

    Google Scholar 

  • Zhang X, Sarmah AK, Bolan NS et al (2016) Effect of aging process on adsorption of diethyl phthalate in soils amended with bamboo biochar. Chemosphere 142:28–34

    Article  CAS  Google Scholar 

  • Zhao HM, Hu RW, Huang HB et al (2017) Enhanced dissipation of DEHP in soil and simultaneously reduced bioaccumulation of DEHP in vegetable using bioaugmentation with exogenous bacteria. Biol Fertil Soils 53:663–675

    Article  CAS  Google Scholar 

  • Zhu FX, Zhu CY, Doyle E et al (2018) Fate of di (2-ethylhexyl) phthalate in different soils and associated bacterial community changes. Sci Total Environ 637–638:460–469

    Article  CAS  Google Scholar 

  • Zhu FX, Zhu CY, Zhou DM, Gao J (2019) Fate of di (2-ethylhexyl) phthalate and its impact on soil bacterial community under aerobic and anaerobic conditions. Chemosphere 216:84–93

    Article  CAS  Google Scholar 

  • Zorníková G, Jarošová A, Hřivna L (2011) Distribution of phthalic acid esters in agricultural plants and soil. Acta Universitatis Agriculturae Et Silviculturae Mendelianae Brunensis 59:233–238

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Key Research Development Plans of Special Project for Site Soils (No. 2018YFC1800605, No. 2018YFC1801001), the Key Research and Development Program of Shandong Province (2019GSF109026), the National Natural Science Foundation of China (No. 41807125), the Shandong Provincial Natural Science Foundation (Nos. ZR2018BD003 and ZR2017MD023), the China Postdoctoral Science Foundation (No.2018M632703), and the Special Funds of Taishan Scholar of Shandong Province, China.

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Author notes

  1. Cui Zhang and Peipei Song contributed equally to this work.

Authors and Affiliations

  1. College of Resources and Environment, Key Laboratory of Agriculture Environment, Shandong Agricultural University, Tai’an, 271018, People’s Republic of China

    Cui Zhang, Peipei Song, Xianxu Li, Jinhua Wang, Lusheng Zhu & Jun Wang

  2. Tai’an City Public Security Bureau in Shandong Province, Tai’an, People’s Republic of China

    Qingbing Xia

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  1. Cui Zhang
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  2. Peipei Song
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  3. Qingbing Xia
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  4. Xianxu Li
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  5. Jinhua Wang
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  6. Lusheng Zhu
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  7. Jun Wang
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Correspondence to Jun Wang.

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Zhang, C., Song, P., Xia, Q. et al. Responses of Microbial Community to Di-(2-ethylhcxyl) Phthalate Contamination in Brown Soil. Bull Environ Contam Toxicol 104, 820–827 (2020). https://doi.org/10.1007/s00128-020-02878-x

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