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Induced pluripotent stem cells for cardiac repair

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Abstract

Myocardial stem cell therapies are emerging as novel therapeutic paradigms for myocardial repair, but are hampered by the lack of sources for autologous human cardiomyocytes. An exciting development in the field of cardiovascular regenerative medicine is the ability to reprogram adult somatic cells into pluripotent stem cell lines (induced pluripotent stem cells, iPSCs) and to coax their differentiation into functional cardiomyocytes. This technology holds great promise for the emerging disciplines of personalized and regenerative medicine, because of the ability to derive patient-specific iPSCs that could potentially elude the immune system. The current review describes the latest techniques of generating iPSCs as well as the methods used to direct their differentiation towards the cardiac lineage. We then detail the unique potential as well as the possible hurdles on the road to clinical utilizing of the iPSCs derived cardiomyocytes in the emerging field of cardiovascular regenerative medicine.

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Abbreviations

CPCs:

Cardiac progenitor cells

EBs:

Embryoid bodies

ESCs:

Embryonic stem cells

hESCs:

Human embryonic stem cells

hiPSCs:

Human-induced pluripotent stem cells

hiPSCs-CMs:

Human-induced pluripotent stem cells-derived cardiomyocytes

hPSCs:

Human pluripotent stem cells

iPSCs:

Induced pluripotent stem cells

miPSCs:

Murine-induced pluripotent stem cells

miRNAs:

MicroRNAs

MSCs:

Mesenchymal stem cells

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Acknowledgments

We apologize to authors whose excellent works were not cited due to space restrictions. This work was supported in part by the Israel Science Foundation [1449/10], by the Israel Science Foundation and Legacy Heritage Foundation (No. 1225/09), by the Lorry Lokey research fund, and by the Nancy and Stephen Grand Philanthropic Fund.

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Authors and Affiliations

  1. The Sohnis Family Research Laboratory for Cardiac Electrophysiology and Regenerative Medicine, The Rappaport Faculty of Medicine and Research Institute, Technion’s Faculty of Medicine, Technion–Israel Institute of Technology, POB 9649, 31096, Haifa, Israel

    Limor Zwi-Dantsis & Lior Gepstein

  2. The Biotechnology Interdisciplinary Unit, Technion–Israel Institute of Technology, Haifa, Israel

    Limor Zwi-Dantsis

Authors
  1. Limor Zwi-Dantsis
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  2. Lior Gepstein
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Correspondence to Lior Gepstein.

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Zwi-Dantsis, L., Gepstein, L. Induced pluripotent stem cells for cardiac repair. Cell. Mol. Life Sci. 69, 3285–3299 (2012). https://doi.org/10.1007/s00018-012-1078-2

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  • DOI: https://doi.org/10.1007/s00018-012-1078-2

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