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Fucoidan increases TNF-α-induced MMP-9 secretion in monocytic cell line U937

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Abstract

Objective

To investigate the effect of fucoidan on the expression of matrix metalloproteinase-9 (MMP-9) from monocytes.

Methods

Human monocytic cell line U937 was purchased from ATCC. During the experiment, FBS-free 1640 was used and U937 was cultivated with 20 ng/ml TNF-α and/or different concentrations of fucoidan for 24 h. RT-PCR experiments were used to determine the MMP-9 mRNA expression. ELISA and gelatin zymography detected MMP-9 amounts and activity in the supernatant. The intracellular level of MMP-9 was assayed by Western blot, and the level of CD44 on the surface was assayed by FACS.

Results

In this study, we showed that pro-inflammatory cytokine TNF-α up-regulated U937 MMP-9 mRNA and protein levels (P < 0.05). Fucoidan can increase the TNF-α-induced MMP-9 secretion from U937 (P < 0.05), but no significant difference was observed in MMP-9 mRNA. The intracellular level of MMP-9 treated with TNF-α and fucoidan was lower (P < 0.05) than that treated with TNF-α alone. In addition, we demonstrated that fucoidan downregulated the surface level of CD44, the main molecule to which MMP-9 attaches.

Conclusions

We demonstrated that fucoidan post-translationally regulated MMP-9 secretion from U937. Reduced intracellular level and decreased membrane attachment may contribute to the increase in MMP-9 secretion.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (no. 30671902 and no. 30872321), and the Natural Science Foundation of Shandong (no. Y2008C02 and no. Y2006C122).

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Authors and Affiliations

  1. Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, 250012, Jinan, Shandong, People’s Republic of China

    Sun Jintang, Feng Alei, Zhang Yun, Hu Weixu, Yang Meixiang & Qu Xun

  2. The School of Stomatology, Shandong University, 250012, Jinan, Shandong, People’s Republic of China

    Sun Shanzhen & Wei Fengcai

Authors
  1. Sun Jintang
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  2. Feng Alei
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  3. Zhang Yun
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  4. Sun Shanzhen
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  5. Hu Weixu
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  6. Yang Meixiang
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  7. Wei Fengcai
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  8. Qu Xun
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Corresponding author

Correspondence to Qu Xun.

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Responsible Editor: G. Wallace.

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Jintang, S., Alei, F., Yun, Z. et al. Fucoidan increases TNF-α-induced MMP-9 secretion in monocytic cell line U937. Inflamm. Res. 59, 271–276 (2010). https://doi.org/10.1007/s00011-009-0095-6

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  • DOI: https://doi.org/10.1007/s00011-009-0095-6

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