Summary
A low passage rat liver cell line demonstrated in vitro growth stimulation when cultured in the presence of serum of homologous, partially hepatectomized rats. After 4-day incubation a 3.25-fold increase in the cell population was observed in cultures supplemented with posthepatectomy serum at a dilution of 1∶10. No response was observed with sham-operated animal serum. Continous cultures of Chang human liver and Don hamster lung cells were not responsive to the posthepatectomy serum. The limitations of tetraphenylboron as a dispersing agent for primary rat liver cells are discussed.
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Supported by Grant 67-7 from the Illinois Division of the American Cancer Society.
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Rutzky, L.P., Taylor, W.G. & Pumper, R.W. In vitro stimulation of rat liver cells by homologous partial hepatectomy serum. In Vitro 7, 1–5 (1971). https://doi.org/10.1007/BF02618997
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DOI: https://doi.org/10.1007/BF02618997