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In vitro stimulation of rat liver cells by homologous partial hepatectomy serum

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Summary

A low passage rat liver cell line demonstrated in vitro growth stimulation when cultured in the presence of serum of homologous, partially hepatectomized rats. After 4-day incubation a 3.25-fold increase in the cell population was observed in cultures supplemented with posthepatectomy serum at a dilution of 1∶10. No response was observed with sham-operated animal serum. Continous cultures of Chang human liver and Don hamster lung cells were not responsive to the posthepatectomy serum. The limitations of tetraphenylboron as a dispersing agent for primary rat liver cells are discussed.

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Author notes

  1. William G. Taylor

    Present address: Tissue Culture Section, Laboratory of Biology, National Cancer Institute, National Institutes of Health, 20014, Bethesda, Md

Authors and Affiliations

  1. Department of Microbiology, University of Ilinois at the Medical Center, 60680, Chicago, Illinois

    Lynne P. Rutzky, William G. Taylor & Robert W. Pumper

Authors
  1. Lynne P. Rutzky
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  2. William G. Taylor
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  3. Robert W. Pumper
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Additional information

Supported by Grant 67-7 from the Illinois Division of the American Cancer Society.

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Rutzky, L.P., Taylor, W.G. & Pumper, R.W. In vitro stimulation of rat liver cells by homologous partial hepatectomy serum. In Vitro 7, 1–5 (1971). https://doi.org/10.1007/BF02618997

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