Summary
Extensive chromosome aberrtions were induced in a diploid monkey cell line designated as BDS-FRhL-2 after exposure to [3H]thymidine ([3H]Tdr) for either 30 or 60 min at a dose of 1 μCi per ml of medium. Cultures exposed to [3H]Tdr for a longer period had significantly larger numbers of aberrations than those exposed for a shorter period. The most common type of aberrations were chromatid breaks. The majority of aberrations were observed in cells which were in contact with [3H]Tdr during S phase, especially the middle S. Cells from cultures of early and late passages exposed to [3H]Tdr were affected to a similar extent when chromosomes were examined. No clear relationship between sites of breakage and intensity of labeling could be established.
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This work was supported by Contract No. FDA-73-179 from the Bureau of Biologics, Food and Drug Administration. DBS-FRhL-2, Division of Biologic Standards-Fetal Rhesus Lung no. 2.
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Huang, C.C., Ninan, T.A. & Petricciani, J.C. Extensive chromosome aberrations caused by [3H]thymidine incorporation in a diploid monkey cell line DBS-FRhL-2. In Vitro 11, 234–238 (1975). https://doi.org/10.1007/BF02616339
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DOI: https://doi.org/10.1007/BF02616339