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An indirect method for absorption rate estimation: Flurothyl-induced seizures

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Abstract

This paper develops a method to estimate a minimal amount of flurothyl necessary to induce the seizures (the seizure threshold). A simple mathematical model is proposed which permits one to determine the drug absorption rate from the amount which has been administered and from the measured latency to onset of seizure. Experimental animal (rats) were exposed to a continuous intake of flurothyl in two different situations: either being alone in the airtight chamber or sharing it in a pair. In the latter case, we assume that the two rats uniformly share the infused drug. Our calculations estimate that approximately 20 μl of flurothyl is necessary to induced twitches, whereas 25 μl of flurothyl is the dose required for the induction of clonic seizures. The model can be used to estimate the threshold amounts of any drug producing obvious behavioral changes irrespective of the route of administration.

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Authors and Affiliations

  1. Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20, Praha 4, Czech Republic

    Petr Lánský & Libor Velíšek

  2. Department of Neurology, K 316, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461, Bronx, NY, U.S.A.

    Jana Velíšková

  3. Department of Pathological Physiology, Third School of Medicine, Charles University, Ke Karlovu 4, CZ-120 00, Praha 2, Czech Republic

    Libor Velíšek

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  1. Petr Lánský
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  2. Jana Velíšková
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  3. Libor Velíšek
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Correspondence to Petr Lánský.

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Lánský, P., Velíšková, J. & Velíšek, L. An indirect method for absorption rate estimation: Flurothyl-induced seizures. Bltn Mathcal Biology 59, 569–579 (1997). https://doi.org/10.1007/BF02459466

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  • DOI: https://doi.org/10.1007/BF02459466

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