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Ultrastructure of endometrial epithelial cells in a three-dimensional cell culture system for human implantation studies

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Abstract

Purpose

A three-dimensional cell culture system imitating normal uterine endometrium has previously been established. To what degree do cultured epithelial cells retain their morphological characteristics as compared to in vivomaterial obtained simultaneously from the same tissue donor.

Results

We found a high degree of similarity between the in vivoand in vitrosituations. The present culture system furthermore imitates the day-to-day morphology of the cycle.

Conclusions

This indicates, that a correct timing of the biopsy tissue is important for future human implantation studies.

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Authors and Affiliations

  1. Department of Obstetrics-Gynecology, Herlev Hospital, University of Copenhagen, DK 2730, Herlev, Denmark

    U. Bentin-Ley, S. Lindenberg & J. Falck Larsen

  2. Department of Pathology, Herlev Hospital, University of Copenhagen, DK 2730, Herlev, Denmark

    T. Horn

  3. Ciconia Fertility Clinic, Frydendahlsvej 5, DK 1809, Frederiksberg C, Denmark

    S. Lindenberg

Authors
  1. U. Bentin-Ley
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  2. S. Lindenberg
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  3. T. Horn
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  4. J. Falck Larsen
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Bentin-Ley, U., Lindenberg, S., Horn, T. et al. Ultrastructure of endometrial epithelial cells in a three-dimensional cell culture system for human implantation studies. J Assist Reprod Genet 12, 632–638 (1995). https://doi.org/10.1007/BF02212588

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  • DOI: https://doi.org/10.1007/BF02212588

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