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A new technique for dissociation of hair follicles into single cells

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Summary

A method is described for the complete dissociation of mouse hair follicles into a suspension of single cells suitable for cell culture. Dermal tissue containing hair follicles is digested sequentially with trypsin and chondroitinase ABC under mild conditions.

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References

  1. R. Frater, J. invest. Derm.64, 235 (1975).

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  2. R. Frater, in preparation.

  3. B. Sylvén, Expl. Cell Res.1, 582 (1950).

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  4. J. P. Kaltenbach, M. H. Kaltenbach andW. B. Lyons, Expl Cell Res.15, 112 (1958).

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Authors and Affiliations

  1. Division of Protein Chemistry, CSIRO, 343 Royal Parade, 3052, Parkville, Victoria, Australia

    R. Frater

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  1. R. Frater
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Frater, R. A new technique for dissociation of hair follicles into single cells. Experientia 32, 675–676 (1976). https://doi.org/10.1007/BF01990230

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  • DOI: https://doi.org/10.1007/BF01990230

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