Skip to main content
SpringerLink
Log in

Enhanced inhibition of RNA synthesis by amanitins in in vitro cultured cells

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The inhibition of RNA synthesis by α-amanitin on vitro cultured cells is very slow. The action of various analogues of the toxin was tested and some of them proved more effective. Moreover pretreatment of cell cultures with DEAE-dextran greatly enhanced the effect of β-amanitin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L. Fiume andTh. Wieland, FEBS Lett.8, 1 (1970).

    Article  CAS  PubMed  Google Scholar 

  2. Th. Wieland andO. Wieland, inMicrobial Toxins (Eds.S. Kadis, A. Ciegler andS. J. Ajl; Academic Press, New York 1972), vol. 8, p. 249.

    Google Scholar 

  3. P. Chambon, inThe Enzymes (Ed.P. D. Boyer; Academic Press, New York and London 1974), vol. 10, p. 261.

    Google Scholar 

  4. F. Stirpe andL. Fiume, Biochem. J.105, 779 (1967).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. L. Montanaro, F. Novello andF. Stirpe, Biochim. biophys. Acta319, 188 (1973).

    Article  CAS  PubMed  Google Scholar 

  6. L. Fiume, M. La Placa andM. Portolani, Sperimentale116, 15 (1966).

    CAS  PubMed  Google Scholar 

  7. A. Mannini Palenzona, F. Costanzo andM. La Placa, Arch. ges. Virusforsch.34, 381 (1971).

    Article  Google Scholar 

  8. L. Fiume andG. Barbanti-Brodano, Experientia30, 76 (1974).

    Article  CAS  Google Scholar 

  9. A. Buku, G. Campadelli-Fiume, L. Fiume andTh. Wieland, FEBS Lett.14, 42 (1971).

    Article  CAS  PubMed  Google Scholar 

  10. H. Faulstich andH. Trischmann, Hoppe-Seyler's Z. physiol. Chem.354, 1395 (1973).

    Article  CAS  Google Scholar 

  11. M. Meilhac, C. Kedinger, P. Chambon, H. Faulstich, M. V. Govindan andTh. Wieland, FEBS Lett.9, 258 (1970).

    Article  Google Scholar 

  12. M. Kuwano andY. Ikehara, Expl Cell Res.82, 454 (1973).

    Article  CAS  Google Scholar 

  13. O. H. Lowry, N. J. Rosebrough, A. L. Farr andJ. R. Randal, J. biol. Chem.193, 265 (1951).

    Article  CAS  PubMed  Google Scholar 

  14. J. M. Hoskins,Virological Procedures (Butterworths, London 1967).

    Google Scholar 

  15. Th. Wieland andW. Boehringer, Justus Liebigs Annln. Chem.635, 178 (1960).

    Article  CAS  Google Scholar 

  16. G. Barbanti-Brodano, P. Swetly andH. Koprowski, J. Virol.6, 644 (1970).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Microbiologia e Virologia, Università di Bologna, Via San Giacomo 12, I-40126, Bologna, Italy

    Laura Foá-Tomasi, F. Costanzo & Gabriella Campadelli-Fiume

Authors
  1. Laura Foá-Tomasi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Costanzo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gabriella Campadelli-Fiume
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgments. We thank ProfessorTh. Wieland and Dr.H. Faulstich (Max-Planck-Institut, Heidelberg) for the generous gift of amanitins. This investigation was supported by grant No. 74.00637.65 from C.N.R. (Roma).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Foá-Tomasi, L., Costanzo, F. & Campadelli-Fiume, G. Enhanced inhibition of RNA synthesis by amanitins in in vitro cultured cells. Experientia 32, 45–46 (1976). https://doi.org/10.1007/BF01932615

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01932615

Keywords

Search

Navigation