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On-line fermenter headspace gas analysis of methanol and ethanol by capillary inlet mass spectrometry

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Biotechnology Techniques

Summary

A new capillary inlet system was used with a magnetic sector mass spectrometer to analyse headspace gas from air-sparged aqueous solutions of methanol and ethanol. The system responded to pulse additions within 2 minutes and gave ≥90% of equilibrium response after 10 minutes. No memory effects or hysteresis were observed. Signal to concentration ratio was linear with alcohol concentrations up to 5 g/L. Liquid ethanol concentration in aerobic yeast fermentation was followed successfully by on-line headspace gas analysis.

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References

  • Bohatka, S., Langer, G., Szilagyi, J. and Berecz, I. (1983). Int. J. Mass. Spectrom. Ion. Phys.,48, 277–280.

    Article  Google Scholar 

  • buckland, B.C., Brix, T., Fastert, H., Gbewonyo, K., Hunt, G. and Jain, D. (1985). Bio/Technology,3, 982–988.

    Article  Google Scholar 

  • Cregg, J.M., Tschopp, J.F., Stillman, C., Siegel, R., Akong, M., Craig, W.S., Buckholz, R.G., Madden, K.R., Kellaris, P.A., Davis, G.R., Smiley, B.L., Cruze, J., Torregrossa, R., veliçelibi, G. and Thill, G.P. (1987). Bio/Technology,5, 479–485.

    Article  Google Scholar 

  • Hamer, G. (1965a). Biotechnol. Bioeng.,7, 199–214.

    Article  Google Scholar 

  • Hamer, G. (1965b). Biotechnol. Bioeng.,7, 215–227.

    Article  Google Scholar 

  • Heinzle, E. (1987). “Mass Spectrometry for On-line Monitoring of Biochemical Processes”, in Advances in Biochemical Engineering/Biotechnology,35, 1–45.

  • Heinzle, E., Furukawa, K., Dunn, I.J. and Bourne, J.R. (1983). Bio/Technology,1, 181–188.

    Article  Google Scholar 

  • Lloyd, D. and James, C.J. (1987). FEMS Microbiol. Letts.42, 27–31.

    Article  Google Scholar 

  • Pungor, E., Perley, C.R., Cooney, C.L. and Weaver, J.C. (1983). Biotechnol. Letts.,2, 409–414.

    Article  Google Scholar 

  • Pungor, E., Schaefer, E.J., Cooney, C.L. and Weaver, J.C. (1983). Eur. J. Appl. Microbiol. Biotechnol.18, 135–140.

    Article  Google Scholar 

  • Williams, L.A. (1983). Biotechnol. Bioeng.,25, 1597–1612.

    Article  Google Scholar 

  • Winter, M.J. (1987). “The application of single detector magnetic sector mass spectrometer systems in fermentation off-gas and liquid analysis”, in Mass Spectrometry in Biotechnological Process Analysis and Control, Eds. Heinzle, E. and Reuss, M., Plenum Press, New York, 17–38.

    Google Scholar 

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Authors and Affiliations

  1. Biological Division, Smith Kline, RIT rue de l'Institut 89, B-1330, Rixensart, Belgium

    J. P. Camelbeeck, D. M. Comberbach, J. Goossens & P. Roelants

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  1. J. P. Camelbeeck
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  2. D. M. Comberbach
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  3. J. Goossens
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  4. P. Roelants
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Camelbeeck, J.P., Comberbach, D.M., Goossens, J. et al. On-line fermenter headspace gas analysis of methanol and ethanol by capillary inlet mass spectrometry. Biotechnol Tech 2, 183–188 (1988). https://doi.org/10.1007/BF01875762

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  • DOI: https://doi.org/10.1007/BF01875762

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