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In vivo development of in vitro fertilized bovine oocytes matured in vivo versus in vitro

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Abstract

In vivo developmental potentials of in vivo and in vitro matured oocytes fertilized in vitro were assessed in cattle. One-cell stages produced from in vivo matured oocytes developed into a pregnancy when transferred to the ampulla part of oviducts of synchronized heifers. In vitro matured oocytes achieved high penetration and cleavage rates but did not develop into pregnancies when transferred to synchronized heifers.

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References

  1. Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF, Dressel MA: Normal development following in vitro fertilization in the cow. Biol Reprod 1982;27:147–158

    PubMed  Google Scholar 

  2. Sirard MA, Lambert RD: Birth of calves after in vitro fertilization using laparoscopy and rabbit oviduct incubation of zygotes. Vet Rec 1986;119:167–169

    PubMed  Google Scholar 

  3. Parrish JJ, Susko-Parrish JL, Leibfried-Rutledge ML, Critser ES, Eyestone WH, First NL: Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 1986;25:591–600

    Google Scholar 

  4. Critser ES, Leibfried-Rutledge ML, Eyestone WH, Northey DL, First NL: Acquisition of developmental competence during maturation in vitro. Theriogenology 1986;25:150

    Google Scholar 

  5. Trounson AO, Willadsen SM, Rowson LEA: Fertilization and development capability of bovine follicular oocytes matured in vitro and in vivo and transferred to the oviducts of rabbits and cows. J Reprod Fertil 1977;51:321–327

    PubMed  Google Scholar 

  6. Hyttel P, Callesen H, Greve T: Ultrastructural features of preovulatory oocyte maturation in superovulated cattle. J Reprod Fertil 1986a;76:645–656

    PubMed  Google Scholar 

  7. Hyttel P, Xu KP, Smith S, Greve T: Ultrastructure of in vitro oocyte maturation in cattle. J Reprod Fertil 1986b;78:615–625

    PubMed  Google Scholar 

  8. Ball GD, Leibfried ML, Lenz RW, Ax RL, Bavister BD, First NL: Factors affecting successful in vitro fertilization of bovine follicular oocytes. Biol Reprod 1983;28:717–725

    PubMed  Google Scholar 

  9. Callesen H, Greve T, Hyttel P: Preovulatory endocrinology and oocyte maturation in superovulated cattle. Theriogenology 1986;25:71–86

    Google Scholar 

  10. Høier R, Callesen H, Greve T: A semiquantitative and rapid method for early prediction of time of ovulation in cattle by means of a radio immuno-sorbent assay of luteinizing hormone (submitted for publication)

  11. Xu KP, Greve T, Smith S, Hyttel P: Chronological changes of bovine follicular oocytes in vitro. Acta Vet Scand 1986;27:505–519

    PubMed  Google Scholar 

  12. Parrish JJ, Susko-Parrish JL, First NL: Effect of heparin and chrondroitin sulfate on the acrosome reaction and fertility of bovine sperm in vitro. Theriogenology 1985;24:537–549

    Google Scholar 

  13. Shea BF, Janzen RE, McAlister R: Recovery and fertilization of bovine follicular oocytes. Theriogenology 1983;19:385–390

    Google Scholar 

  14. Xu KP, Greve T, Callesen H, Hyttel P: Pregnancy resulting from cattle oocytes matured and fertilized in vitro. J Reprod Fertil 1987 (to appear)

  15. Newcomb R, Christie WB, Rowson LEA: Birth of calves after in vivo fertilization of oocytes removed from follicles and matured in vitro. Vet Rec 1978;102:461–462

    PubMed  Google Scholar 

  16. Moor RM, Trounson AO: Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent developmental capacity. J Reprod Fertil 1977;49:101–109

    PubMed  Google Scholar 

  17. Staigmiller RB, Moor RM: Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicle. Gamete Res 9:221–229

  18. Moor RM, Osborn JC, Cran DG, Walters DE: Selective effect of gonadotrophins on cell coupling, nuclear maturation and protein synthesis in mammalian oocytes. J Embryol Exp Morphol 1981;61:347–365

    PubMed  Google Scholar 

  19. King WA, Bousquet D, Greve T, Goff AK: Meiosis in bovine oocytes matured in vitro and in vivo. Acta Vet Scand 1986;27:267–279

    PubMed  Google Scholar 

  20. Hyttel P, Greve T, Xu KP, Callesen H: Ultrastructural aspects of bovine fertilization in vivo and in vitro.In Proceedings of Symposium: Application of Egg and Embryo Technologies to Domestic Animals, T Greve (ed). Copenhagen, Department of Animal Reproduction, Royal Veterinary and Agricultural University, 1987, pp 41–44

    Google Scholar 

  21. Tesarik J, Kopency V, Plachot M, Mandelbaum J: Activation of nucleolar and extranucleolar RNA synthesis and changes in the ribosomal content of human embryos developing in vitro. J Reprod Fertil 1986;78:463–470

    PubMed  Google Scholar 

  22. Eyestone WH, First NL: A study of the 8- to 16-cell developmental block in bovine embryos cultured in vitro. Theriogenology 1986;25:152

    Google Scholar 

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Greve, T., Xu, K.P., Callesen, H. et al. In vivo development of in vitro fertilized bovine oocytes matured in vivo versus in vitro. J Assist Reprod Genet 4, 281–285 (1987). https://doi.org/10.1007/BF01555205

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  • DOI: https://doi.org/10.1007/BF01555205

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