Summary
A surgical procedure to expose the arterial bifurcation at the base of the rat brain was developed without sacrificing the animal. Using this technique, visualization of flow in and around the induced cerebral aneurysm was achieved by detecting and following fluorescent particles in the blood stream. Cerebral aneurysms were produced by ligating one common carotid artery, inducing experimental hypertension and feeding them with beta-aminopropionitrile.
Flow studies of the arterial bifurcation with an early aneurysmal formation showed that there were spiral flows proximal and distal to the bifurcation. This was the first direct visualization of the actual flow in and around cerebral aneurysms in a vital state. This technology can add further information on the development, growth and rupture of cerebral aneurysms.
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References
Fukushima T, Homma T, Azuma T, Harakawa K (1987) Characteristics of secondary flow in steady and pulsatile flows through a symmetrical bifurcation. Biorheology 24: 3–12
Hale SL, Vivaldi MT, Kloner RA (1986) Fluorescent microspheres: a new tool for visualization of ischaemic myocardium in rats. Am J Physiol 251: 863–868
Hashimoto N, Handa H, Nagata I, Hazama F (1984) Animal model of cerebral aneurysms: pathology and pathogenesis of induced cerebral aneurysms in rats. Neurol Res 6: 32–40
Hashimoto N, Kim C, Kikuchi H, Kojima M, Kang Y, Hazama F (1987) Experimental induction of cerebral aneurysms in monkeys. J Neurosurg 67: 903–905
Hazama F, Kataoka H, Yamada E, Kayembe K, Hashimoto N, Kojima M, Kim G (1986) Early changes of experimentally induced cerebral aneurysms in rats. Light microscopic study. Am J Pathol 124: 399–404
Karino T, Motomiya M (1983) Flow visualization in isolated parent natural blood vessels. Biorheology 20: 119–127
Kim C, Kikuchi H, Hashimoto N, Kojima M, Hazama F (1988) Involvement of internal elastic lamina in development of induced cerebral aneurysms in rats. Stroke 19: 507–511
Kojima M, Handa H, Hashimoto N, Kim C, Hazama F (1986) Early changes of experimentally induced cerebral aneurysms in rats. Scanning electron microscopic study. Stroke 17: 835–841
McDonagh PF, Niven AT, Roberts D (1984) Direct visualization for pharmacologic and physiologic studies. Microvasc Res 28: 180–196
Nakatani H, Hashimoto N, Kang Y, Yamazoe N, Kikuchi H, Yamaguchi S, Niimi H (1991) Cerebral blood flow patterns at major vessel bifurcations and aneurysms. J Neurosurg 74: 258–262
Niimi H, Kawano Y, Sugiyama I (1984) Structure of blood flow through a curved vessel with an aneurysm. Biorheology 21: 603–615
Nuttall AL (1987) Techniques for the observation and measurement of red blood cell velocity in vessels of the guinea pig cochlea. Heart Res 27: 111–119
Stehbens WE (1975) Flow in glass models of arterial bifurcations and berry aneurysms at low Reynolds numbers. J Exp Physiol 60: 181–192
Steiger HJ, Poll A, Liepsch DW,et al (1987) Basic flow structure in saccular aneurysms: a flow visualization study. Heart Vessels 3: 55–65
Steiger HJ (1990) Pathophysiology of development and rupture of cerebral aneurysms. Acta Neurochir (Wien) [Suppl] 48
Tangelder GJ, Slaaf DW, Muijtjens AMM, Ats T, Mirjam GA, Oude Egbrink, Reneman RS (1986) Velocity profiles of blood platelets and red blood cells flowing in arterioles of the rabbit mesentery. Circ Res 59: 505–514
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This study was supported by Grant-in Aids for Scientific Research from the Ministry of Education, Science and Culture of Japan (02670626, 02452120).
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Nakatani, H., Hashimoto, N., Kikuchi, H. et al. In vivo flow visualization of induced saccular cerebral aneurysms in rats. Acta neurochir 122, 244–249 (1993). https://doi.org/10.1007/BF01405537
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DOI: https://doi.org/10.1007/BF01405537