Summary
Interphase cells ofDictyota dichotoma (Hudson) Lamour. lack cortical microtubules (Mts) but display an impressive network of cytoplasmic microtubules (c-Mts). These are focussed on two opposed perinuclear centriolar sites where centrin or a centrin-homologue is localized. Some of the Mts surround the nucleus, but the majority traverse the cytoplasm as bundles variously directed towards the plasmalemma. In apical cells, and to a lesser extent in the square or slightly elongated meristematic cells, Mts are more or less evenly arranged. In elongated cells they form thick bundles longitudinally traversing the cytoplasm; a pattern maintained in differentiated cells. In early prophase the non-perinuclear Mts disappear but by late prophase a bi-astral arrangement of short Mts is observed. They enter polar nuclear depressions and attach to differentiated regions of the nuclear envelope where polar gaps open. By metaphase the spindle Mts converge on the centrioles at the polar gaps. At anaphase, interzonal Mts are evident and the asters start to reassemble. After telophase disruption of the interzonal Mts, the daughter nuclei approach each other, but move apart again before cytokinesis. The latter movement keeps pace with the development of two interdigitating Mt systems, ensheathing both daughter nuclei. The partition membrane “bisects” this Mt “cage”. Between telophase and cytokinesis the centrosomes separate, finally occupying opposed perinuclear sites. New Mts arise at the new centrosomes, some terminating on the consolidating partition membrane. Our data show thatD. dichotoma vegetative cells display a prominent cytoplasmic Mt cytoskeleton, which undergoes continual, but definite, change in organization during the cell cycle.
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References
Aitchison WA, Brown DL (1986) Duplication of the flagellar apparatus and cytoskeletal microtubule system in the algaPolytomella. Cell Motil Cytoskeleton 6: 122–127
Bakker RP, Lokhorst GM (1987) Ultrastructure of mitosis and cytokinesis inZygnema sp. (Zygnematales, Chlorophyta). Protoplasma 138: 105–118
Baskin TI, Cande WZ (1990) The structure and function of the mitotic spindle in flowering plants. Annu Rev Plant Physiol Plant Mol Biol 41: 277–315
Baron AT, Salisbury JL (1988) Identification and localization of a novel, cytoskeletal, centrosome-associated protein in PtK2 cells. J Cell Biol 107: 2669–2678
Bech-Hansen CW, Fowke LC (1972) Mitosis inMougeotia sp. Can J Bot 50: 1811–1816
Bouck GB (1965) Fine structure and organelle associations in brown algae. J Cell Biol 26: 523–537
Brawley SH, Quatrano RS, Wetherbee R (1977) Fine-structural studies of the gametes and embryo ofFucus vesiculosus L. (Phaeophyta). III Cytokinesis and the multicellular embryo. J Cell Sci 24: 275–294
Davies JM, Ferrier NC, Johnston CS (1973) The ultrastructure of the meristoderm cells of the hapteron ofLaminaria. J Mar Biol Assoc UK 53: 237–246
Fowke LC, Pickett-Heaps JD (1969) Cell division inSpirogyra. I. Mitosis. J Phycol 5: 240–259
Galatis B, Katsaros C, Mitrakos K (1973) Ultrastructure of the mitotic apparatus inDictyota dichotoma (Lamour). Rapp Comm Int Mer Medit 22: 53–54
Galway ME, Hardham AR (1991) Immunofluorescent localization of microtubules throughout the cell cycle in the green algaMougeotia (Zygnemataceae). Amer J Bot 78: 451–461
Hallam ND, Luff SE (1988) The fixation and infiltration of larger brown algae (Phaeophyta) for electron microscopy. Br Phycol J 23: 337–346
Höhfeld I, Otten J, Melkonian M (1988) Contractile eucaryotic flagella: centrin is involved. Protoplasma 147: 16–24
Hogetsu T (1987) Re-formation and ordering of wall microtubules inSpirogyra cells. Plant Cell Physiol 28: 875–883
—, Oshima Y (1985) Immunofluorescence microscopy of microtubule arrangement inClosterium acerosum (Schrank) Ehrenberg. Planta 166: 169–175
Katsaros C (1980) An ultrastructural study on the morphogenesis of the thallus of five brown algal species. PhD Thesis, Athens, Greece
—, Galatis B (1985) Ultrastructural studies on thallus development inDictyota dichotoma (Phaeophyta, Dictyotales). Br Phycol J 20: 263–276
— — (1988) Thallus development inDictyopteris membranacea (Phaeophyta, Dictyotales). Br Phycol J 23: 71–88
— — (1990) Thallus development inHalopteris filicina (Phaeophyceae, Sphacelariales). Br Phycol J 25: 63–74
— —, Mitrakos K (1983) Fine structural studies on the interphase and dividing apical cells ofSphacelaria tribuloides (Phaeophyta). J Phycol 19: 16–30
—, Kreimer G, Melkonian M (1991) Localization of tubulin and a centrin-homologue in vegetative cells and developing gametangia ofEctocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales). A combined immunofluorescence and confocal laser scanning microscope study. Bot Acta 104: 87–92
La Claire JW II (1982) Light and electron microscopic studies on growth and reproduction inCutleria (Phaeophyta). III. Nuclear division in the trichothalic meristem ofC. cylindrica. Phycologia 21: 273–287
— (1987) Microtubule cytoskeleton in intact and wounded coenocytic green algae. Planta 171: 30–42
—, West JA (1979) Light-and electron-microscopic studies of growth and reproduction inCutleria (Phaeophyta). II. Gametogenesis in the male plant ofC. hancockii. Protoplasma 101: 247–267
Lloyd CW (1987) The plant cytoskeleton: the impact of fluorescence microscopy. Annu Rev Plant Physiol 38: 119–139
Markey DR, Wilce RT (1975) The ultrastructure of reproduction in the brown algaPylaiella littoralis. I. Mitosis and cytokinesis in the plurilocular gametangia. Protoplasma 85: 219–241
— — (1976) The ultrastructure of reproduction of the brown algaPylaiella littoralis. II. Zoosporogenesis in the unilocular sporangia. Protoplasma 88: 147–173
Mazia D (1987) The chromosome cycle and the centrosome cycle in the mitotic cycle. Int Rev Cytol 100: 49–92
Melkonian M (1989) Centrin-mediated motility: a novel cell motility mechanism in eukaryotic cells. Bot Acta 102: 3–4
—, Beech PL, Katsaros C, Schulze D (1992) Centrin-mediated cell motility in algae. In: Melkonian M (ed) Algal cell motility. Chapman and Hall, New York, pp 19–2217
Motomura T (1991) Immunofluorescence microscopy of fertilization and parthenogenesis inLaminaria angustata (Phaeophyta). J Phycol 27: 248–257
—, Sakai Y (1985) Ultrastructural studies on nuclear division in sporophyte ofCarpomitra cabrerae (Clemente) Kützing (Phaeophyta, Sporochnales). Jpn J Phycol 33: 199–209
Neushul M, Dahl AL (1972) Ultrastructural studies of brown algal nuclei. Amer J Bot 59: 401–410
Osborn M, Born T, Koitsch H, Weber K (1978) Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules, and tonofilaments. Cell 14: 477–488
Pickett-Heaps JD (1975) Green algae: structure, reproduction, and evolution in selected genera. Sinauer, Sunderland, MA
Rawlence DJ (1973) Some aspects of the ultrastructure ofAscophyllum nodosum (L.) Le Jolis (Phaeophyceae, Fucales) including observations on cell plate formation. Phycologia 12: 17–28
Salisbury J, Baron A, Surek B, Melkonian M (1984) Striated flagellar roots: isolation and partial characterization of a calcium-modulated contractile organelle. J Cell Biol 99: 962–970
— —, Coling DE, Martindale VE, Sanders MA (1986) Calcium-modulated contractile proteins associated with the eucaryotic centrosome. Cell Motil Cytoskeleton 6: 193–197
Seagull RW (1989) The plant cytoskeleton. CRC Crit Rev Plant Sci 82: 131–167
Segaar PJ (1989) Dynamics of the microtubular cytoskeleton in the green algaAgaphanochaete magna (Chlorophyta). II. The cortical cytoskeleton, astral microtubules, and spindle during the division cycle. Can J Bot 67: 239–246
— (1990) The flagellar apparatus and temporary centriole-associated microtubule systems at the interphase-mitosis transition in the green algaGloeomonas kupfferi: an example of the spatiotemporal flexibility of microtubule organizing centres. Acta Bot Neerl 39: 29–42
—, Lokhorst GM (1987) Cell division in the green algaUlothrix palusalsa (Ulvophyceae, Chlorophyta): a combined immunofluorescence and transmission electron microscopy study. Phycologia 26: 100–110
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Katsaros, C., Galatis, B. Immunofluorescence and electron microscopic studies of microtubule organization during the cell cycle ofDictyota dichotoma (Phaeophyta, Dictyotales). Protoplasma 169, 75–84 (1992). https://doi.org/10.1007/BF01343372
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DOI: https://doi.org/10.1007/BF01343372