Summary
Northway virus replication has been detected in salivary glands of wild-caughtCuliseta inornata andAedes communis mosquitoes from the western Canadian Arctic after incubation at 4° C for 9 to 11 months, and after incubation at 13° C for 3 to 4 months after they received virus by oral ingestion or intrathoracic injection.Aedes hexodontus supported Northway virus replication after incubation at 13° C for one month after intrathoracic injection.Aedes aegypti supported Northway virus replication after incubation at 13° C or 23° C for 6 to 28 days following intrathoracic injection. A larval isolate of California encephalitis virus (snowshoe hare subtype) multiplied in all 3 species of arctic mosquito after incubation at 13° C for 1 to 3 months after virus was administered by oral ingestion or intrathoracic injection. Virus was detected in salivary glands ofCs. inornata after 329 days incubation at 4° C after intrathoracic injection. Bunyavirus antigens in salivary glands of arctic and domestic mosquitoes were detected by the glucose oxidase immunoenzyme technique somewhat less frequently than by assay for virus infectivity.
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Supported through the Medical Research Council Canada Grant MT-2811 and the National Research Council Canada Contract 031-604.
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McLean, D.M., Grass, P.N., Judd, B.D. et al. Bunyavirus development in arctic andAedes aegypti mosquitoes as revealed by glucose oxidase staining and immunofluorescence. Archives of Virology 62, 313–322 (1979). https://doi.org/10.1007/BF01318105
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DOI: https://doi.org/10.1007/BF01318105