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The proteins of adenovirus type 5

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Summary

Adenovirus type 5 was degraded by 8m urea to release three readily identifiable internal components and three species of surface hexon component each of which appeared to be associated with minor constituents which were lacking in the “soluble” hexon antigens. The vertex projection was destroyed.

All components were recognized serologically. Whole virus antiserum appeared to contain antibody to both external and internal components. Any breakdown product that reacted with this antiserum and not “soluble” hexon antiserum was regarded as an internal component.

The major internal component was estimated to be 170Å×23Å and of molecular weight (MW) 280,000. Two minor internal components were of approximate MW 5,000.

The individual hexons, as well as “soluble” hexons were estimated to be 160Å×33Å and of MW 380,000. The three species were ascribed various positions in the virus particle.

  1. 1.

    Hexons surrounding the vertex capsomere — peripentons.

  2. 2.

    Hexons that lie along the adjacent sides — adjacent hexons.

  3. 3.

    Hexons occurring in triangular facets of six lying in the one plane — hexons proper.

It is suggested that the central hole of the hexons plays a role in attaching them to the virus particle. This attachment may be mediated by the major internal component which could occur as a radial array around the DNA base structure.

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Author notes

  1. K. F. Shortridge

    Present address: Department of Bacteriology, University College Hospital Medical School, London, England

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  1. Department of Bacteriology, University College Hospital Medical School, London, England

    F. Biddle

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Shortridge, K.F., Biddle, F. The proteins of adenovirus type 5. Archiv f Virusforschung 29, 1–24 (1970). https://doi.org/10.1007/BF01253875

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  • DOI: https://doi.org/10.1007/BF01253875

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