Summary
Adenovirus type 5 was degraded by 8m urea to release three readily identifiable internal components and three species of surface hexon component each of which appeared to be associated with minor constituents which were lacking in the “soluble” hexon antigens. The vertex projection was destroyed.
All components were recognized serologically. Whole virus antiserum appeared to contain antibody to both external and internal components. Any breakdown product that reacted with this antiserum and not “soluble” hexon antiserum was regarded as an internal component.
The major internal component was estimated to be 170Å×23Å and of molecular weight (MW) 280,000. Two minor internal components were of approximate MW 5,000.
The individual hexons, as well as “soluble” hexons were estimated to be 160Å×33Å and of MW 380,000. The three species were ascribed various positions in the virus particle.
-
1.
Hexons surrounding the vertex capsomere — peripentons.
-
2.
Hexons that lie along the adjacent sides — adjacent hexons.
-
3.
Hexons occurring in triangular facets of six lying in the one plane — hexons proper.
It is suggested that the central hole of the hexons plays a role in attaching them to the virus particle. This attachment may be mediated by the major internal component which could occur as a radial array around the DNA base structure.
Similar content being viewed by others
References
Biddle, F., andK. F. Shortridge: Immunological cross-reactions of influenza virus inhibitors. Brit. J. exp. Path.48, 285–293 (1967).
Bradstreet, C. M., andC. E. D. Taylor: Technique of complement fixation test applicable to the diagnosis of virus diseases. Mth. Bull. Minist. Hlth Lab. Serv.21, 96–104 (1962).
Edsall, J. T.: In: The Proteins (H. Neurath andK. Bailey, eds.), Vol. 1, Part B, Chapter 7. New York: Academic Press (1953).
Godman, G. C., C. Morgan, P. M. Breitenfeld, andH. M. Rose: A correlative study by electron and light microscopy of the development of type 5 adenovirus. J. exp. Med.112, 383–402 (1960).
Hershey, A. D.: An upper limit to the protein content of the germinal substance of bacteriophage T2. Virology1, 108–127 (1955).
Johnson, M. W., andR. Markham: Nature of the polyamine in plant viruses. Virology17, 276–281 (1962).
Kjellén, L., andH. G. Pereira: Role of adenovirus antigens in the induction of virus neutralizing antibody. J. gen. Virol.2, 177–185 (1968).
Laver, W. G., H. G. Pereira, W. C. Russell, andR. C. Valentine: Isolation of an internal component from adenovirus type 5. J. molec. Biol.37, 379–386 (1968).
Levine, L., J. L. Barlow, andH. Van Vunakis: An internal protein in T2 and T4 bacteriophages. Virology6, 702–717 (1958).
Maizel, J. V., D. O. White, andM. D. Scharff: The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology36, 115–125 (1968a).
Maizel, J. V., D. O. White, andM.D. Scharff: The polypeptides of adenovirus. I. Soluble proteins, cores, top components and the structure of the virion. Virology36, 126–136 (1968b).
Minagawa, T.: Some characteristics of the internal protein of phage T2. Virology13, 515–527 (1961).
Pereira, H. G.: Antigenic structure of non-infectious adenovirus materials. Nature (Lond.)186, 571–572 (1960).
Petterson, U., L. Philipson, andS. Höglund: Structural proteins of adenoviruses. I. Purification and characterization of adenovirus type 2 hexon antigen. Virology33, 575–590 (1967).
Rosen, L.: Haemagglutination by adenovirus. Virology5, 574–577.
Russell, W. C., andY. Becker: A maturation factor for adenovirus. Virology35, 18–27 (1968).
Russell, W. C., andB. E. Knight: Evidence for a new antigen within the adenovirus capsid. J. gen. Virol.1, 523–528 (1967).
Russell, W. C., R. C. Valentine, andH. G. Pereira: The effect of heat on the anatomy of the adenovirus. J. gen. Virol.1, 509–522 (1967).
Sephadex — Gel Filtration in Theory and Practice. Pharmacia fine chemicals, Uppsala, Sweden (1966).
Shortridge, K. F., andF. Biddle: A human serum inhibitor of adenovirus haemagglutination. Arch. ges. Virusforsch.25, 148–159 (1968).
Siegel, L. M., andK. J. Monty: Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulphite and hydroxylamine reductases. Biochim. biophys. Acta (Amst.)112, 346–362 (1966).
Smith, K. O., W. C. Gehle, andM. D. Trousdale: Architecture of the adenovirus capsid. J. Bact.90, 254–261 (1965).
Valentine, R. C., andH. G. Pereira: Antigens and structure of the adenovirus. J. molec. Biol.13, 13–20 (1965).
Werner, E. A.: The constitution of carbamides: Part XV. A delicate and trust-worthy test for the recognition of cyanic acid. J. Chem. Soc.123, 2577–2579 (1923).
Wilcox, W. C., H. S. Ginsberg, andT. F. Anderson: Structure of type 5 adenovirus. II. Fine structure of virus subunits. Morphological relationship of structural subunits to virus specific soluble antigens from infected cells. J. exp. Med.118, 307–314 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shortridge, K.F., Biddle, F. The proteins of adenovirus type 5. Archiv f Virusforschung 29, 1–24 (1970). https://doi.org/10.1007/BF01253875
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01253875