Summary
The Bucyrus strain of equine arteritis virus (EAV) was grown in BHK-21, primary rabbit kidney and primary horse testicle cells. Viral yields from these cells produced distinct plaques in BHK-21 cells. The relationship between virus concentration and plaque count was linear. Addition of 50 μg/ml DEAE-Dextran to the diluent of EAV gave a maximal number of plaques. Differences in end-point titers of infectivity indicated that BHK-21 cells were as sensitive to EAV as rabbit kidney or horse testicle cells.
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Hyllseth, B. A plaque assay of equine arteritis virus in BHK-21 cells. Archiv f Virusforschung 28, 26–33 (1969). https://doi.org/10.1007/BF01250842
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DOI: https://doi.org/10.1007/BF01250842