Summary
When the temperature of air-equilibrated water is varied, water O2 concentration changes broadly because the O2 solubility coefficient is temperature-dependent. With decreasing temperature the air-equilibrated water remains ‘normoxic’ as to O2 tension,P O 2, but becomes hyperoxic as to O2 concentration,C O 2. A change of temperature, or a change of water oxygenation affects the hemolymph acid-base balance, ABB, of the crayfishAstacus leptodactylus. The present experiments were designed to test the effect of temperature changes on hemolymph ABB at either constant waterP O 2 or at constantC O 2.
In vivo experimental results show that an increase of pH with a fall in temperature at constantP O 2 is offset by the concomitant increase of waterC O 2. At constantP O 2, the pH vs temperature slope was −0.008 pH unit·°C−1; at constantC O 2 the mean slope was −0.014 pH unit·°C−1.
In conclusion, an interpretation of the effect of ambient temperature variations on the ABB of the crayfish must take into account the fact that there are concomitant changes of the water oxygenation which has an effect per se on hemolymph ABB. The temperature-induced pH change of the hemolymph is about 40% less at constantP O 2 than at constantC O 2.
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Abbreviations
- ABB:
-
acid-base balance
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Dejours, P., Armand, J. Acid-base balance of crayfish hemolymph: Effects of simultaneous changes of ambient temperature and water oxygenation. J Comp Physiol B 149, 463–468 (1983). https://doi.org/10.1007/BF00690004
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DOI: https://doi.org/10.1007/BF00690004