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Bean pathogenesis-related (PR) proteins deduced from elicitor-induced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergens

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Summary

We have searched for induced transcripts in a cDNA library derived from bean cell suspension cultures treated with an elicitor fromColletotrichum lindemuthianum. Six independently isolated cDNAs corresponding to rapidly induced small mRNAs have been classified by their DNA sequence and slightly different induction behaviour into two groups. 5′- and 3′-untranslated regions exhibit little similarity, but the deduced small acidic proteins designated PvPR1 and PvPR2 are 89% identical. No relationship was found with the well-characterized PR1 proteins from tobacco. However, the PvPR proteins are closely related to pI49 in pea (64% identity), pSTH2 in potato (41 % identity) and PcPR1-1 in parsley (39% identity), which are also induced in response to elicitor or microbial attack. Moreover, a major pollen allergen in birch (Betv1) has a 44% identity with PvPR1 proteins. These similarities establish a ubiquitous class of conserved defense-related proteins and suggest a common yet still unknown function. Southern blot analysis indicates that PvPR protein gene organization is highly complex with an estimated copy number of more than 12 genes.

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Author notes

  1. Claude Grand

    Present address: RAGT, 18 rue de Séguret Sainerie, B.P. 326, F-12003, Rodez, France

Authors and Affiliations

  1. Institut für Planzenphysiologie 260, Universität Hohenheim, P.O. Box 700562, D-7000, Stuttgart 70, Germany

    Michael H. Walter, Jan-Wei Liu & Dieter Hess

  2. The Salk Institute for Biological Studies, Plant Biology Laboratory, P.O. Box 85800, 92138-9216, San Diego, CA, USA

    Michael H. Walter, Claude Grand & Christopher J. Lamb

Authors
  1. Michael H. Walter
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  2. Jan-Wei Liu
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  3. Claude Grand
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  4. Christopher J. Lamb
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  5. Dieter Hess
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Communicated by R.G. Herrmann

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Walter, M.H., Liu, JW., Grand, C. et al. Bean pathogenesis-related (PR) proteins deduced from elicitor-induced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergens. Mol Gen Genet 222, 353–360 (1990). https://doi.org/10.1007/BF00633840

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  • DOI: https://doi.org/10.1007/BF00633840

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