Summary
In the isolated taenia coli of the guinea-pig, a cooling to 13°C was necessary to suppress the spontaneous activity completely. Under these conditions, regular spikes (intracellular measurements) with contractions can be produced by electrical stimulation.
The Ca-content of unstimulated preparations, determined as a basis for fluxmeasurements to test the hypothesis of calcium spikes, was constant during the observation period, 1–3 h after the beginning of the experiment, (3.1 mmole/kg wet weight as compared to 1.9 mmole/kg in the fresh tissue). In the resting state, 63% of the total Ca was exchangeable with45Ca in 2 h. The exchange could be expressed as the sum of two exponential components with half-times of 0.4 min and 4 min. This allows one to interpret the ion movements, by means of a three compartment model, as transport processes between the organ bath (fraction,S 3) and the extracellular space (fractionS 2) as well as betweenS 2 and a cellular fraction (S 1).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Bauer, H., Goodford, P. J., Hüter, J.: The calcium content and45calcium uptake of the smooth muscle of the guinea-pig taenia coli. J. Physiol. (Lond.)176, 163–179 (1965).
Brading, A., Bülbring, E., Tomita, T.: The effect of sodium and calcium on the action potential of the smooth muscle of the guinea-pig taenia coli. J. Physiol. (Lond.)200, 637–654 (1969).
—, Jones, A. W.: Distribution and kinetics of Co-EDTA in smooth muscle, and its use as an extracellular marker. J. Physiol. (Lond.)200, 387–401 (1969).
van Breemen, C., Daniel, E. E., van Breemen, D.: Calcium distribution and exchange in the rat uterus. J. gen. Physiol.49, 1265–1297 (1966).
Bülbring, E., Kuriyama, H.: Effects of changes in the external sodium and calcium concentrations on the spontaneous electrical activity in smooth muscle of guinea-pig taenia coli. J. Physiol. (Lond.)166, 29–58 (1963a).
——: The effect of adrenaline on the smooth muscle of guinea-pig taenia coli in relation to the degree of stretch. J. Physiol. (Lond.)169, 198–212 (1963b).
Chujyo, N., Holland, W. C.: Potassium-induced contracture and calcium exchange in the guinea pig's taenia coli. Amer. J. Physiol.205, 94–100 (1963).
Golenhofen, K., Loh, D. v.: Elektrophysiologische Untersuchungen zur normalen Spontanaktivität der isolierten Taenia coli des Meerschweinchens. Pflügers Arch.314, 312–328 (1970).
Goodford, P. J.: The loss of radioactive45calcium from the smooth muscle of the guinea-pig taenia coli. J. Physiol. (Lond.)176, 180–190 (1965).
—: An interaction between potassium and sodium in the smooth muscle of the guinea-pig taenia coli. J. Physiol. (Lond.)186, 11–26 (1966).
Goodford, P. J.: The radioactive cobalticyanide ion60Co (CN) 3−6 as an extracellular marker in smooth muscle. J. Physiol. (Lond.)191, 111 P (1967).
—, Hermansen, K.: Sodium and potassium movements in the unstriated muscle of the guinea-pig taenia coli. J. Physiol. (Lond.)158, 426–448 (1961).
—, Leach, E. H.: The extracellular space of the smooth muscle of the guinea-pig taenia coli. J. Physiol. (Lond.)186, 1–10 (1966).
Haas, H. G.: Zur Kinetik des Ionenaustausches in mehrphasigen Systemen. Pflügers Arch. ges. Physiol.275, 347–357 (1962).
—, Glitsch, H. G.: Kalium-Fluxe am Vorhof des Froschherzens. Pflügers Arch. ges. Physiol.275, 358–375 (1962).
Hagiwara, S., Naka, K.-I.: The initiation of spike potential in barnacle muscle fibers under low intracellular Ca++. J. gen. Physiol.48, 141–162 (1965).
Hattingberg, M. v., Kuschinsky, G., Rahn, K. H.: Der Einfluß von Pharmaka auf Calciumgehalt und45Calciumaustausch der glatten Muskulatur der Taenia coli vom Meerschweinchen. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.253, 438–443 (1966).
Lammel, E., Golenhofen, K.: Messungen der45Ca-Aufnahme an intestinaler glatter Muskulatur zur Hypothese eines von Ca-Ionen getragenen Aktionsstromes. Pflügers Arch.329, 269–282 (1971).
——, Schaumlöffel, E.: Ca++-Influx an der isolierten Taenia coli des Meerschweinchens bei 13°C, in Ruhe und bei elektrischer Reizung. Pflügers Arch.307, R85 (1969).
Loh, D. v., Golenhofen, K.: Temperatureinflüsse auf die Spontanaktivität der isolierten Taenia coli vom Meerschweinchen. Pflügers Arch.318, 35–50 (1970).
Pfaffmann, M., Holland, W.: Effects of ouabain and 2,4-dinitrophenol on the Ca exchange in taenia coli. Amer. J. Physiol.211, 400–402 (1966).
Schatzmann, H. J.: Calciumaufnahme und Abgabe am Darmmuskel des Meerschweinchens. Pflügers Arch. ges. Physiol.274, 295–310 (1961).
Shimo, Y., Porter, M. T., Holland, W. C.: Effects of ouabain on calcium exchange and tension in taenia coli. Amer. J. Physiol.214, 804–807 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lammel, E., Golenhofen, K. Erregbarkeit und Ca-Austausch intestinaler glatter Muskulatur (Taenia coli des Meerschweinchens) unterhalb der kritischen Temperatur für die Spontanaktivität. Pflugers Arch. 329, 258–268 (1971). https://doi.org/10.1007/BF00586619
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00586619