Summary
pH sensitivity of cells located at the ventral surface of the medulla ablongata was examined in a thin brain slice of the cat in vitro and the following results were obtained: (1) transmembrane potential of the surface cells located in the area medial to the XIIth cranial nerve was reduced slightly by application of low pH solution; (2) In the rostral part of the area medial to the XIIth cranial nerve regular neuronal discharges could be observed extracellularly. The rate of firing of these cells was increased by lowering the external pH.
These results were considered to support the idea that H+ receptor cells may exist in the surface layer of the ventral medulla.
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References
Brookhart, J. M., Moruzzi, G., Snider, R. S.: Spike discharges of single units in the cerebellar cortex. J. Neurophysiol.13, 465–486 (1950)
Brooks, C. McC., Eccles, J. C.: A study of the effects of anaesthesia and asphyxia on the monosynaptic pathway through the pinal cord. J. Neurophysiol.10, 349–360 (1947)
Brown, A. M., Berman, P. R.: Mechanism of excitation of Aplysia neurons by carbon dioxide. J. gen. Physiol.56, 543–558 (1970)
Caspers, H., Speckmann, E. J.: CerebralP O2,P CO2and pH: changes during convulsive activity and their significance for spontaneous arrest of seizures. Epilepsia13, 699–725 (1972)
Chalazonitis, N.: Effects of changes in PCO2and PO2on rhythmic potentials from giant neurons. Ann. N.Y. Acad. Sci.109, 451–479 (1963)
Eyzaguirre, C., Koyano, H.: Effects of hypoxia, hypercapnea and pH on the chemoreceptor activity of the carotid body in vitro. J. Physiol. (Lond.)178, 385–409 (1965)
Fukuda, Y., Honda, Y.: pH-sensitive cells at ventro-lateral surface of rat medulla oblongata. Nature (Lond.) New Biol.256, 317–318 (1975)
Gill, P. K., Kuno, M.: Properties of phrenic motoneurones. J. Physiol. (Lond.)168, 258–273 (1963)
Hori, T., Roth, G. I., Yamamoto, Y. S.: Respiratory sensitivity of rat brain-stem surface to chemical stimuli. J. appl. Physiol.28, 721–724 (1970)
Kille, J. F., Dermiezel, R., Willenberg, I. M.: Darstellung einzelner Neurone in vitro an der ventralen Oberfläche der Medulla oblongata. 70. Verh. Anat. Ges. (in press, 1975)
Krynjevic, K., Randic, M., Siesjö, B. K.: Cortical CO2 tension and neuronal excitability. J. Physiol. (Lond.)176, 105–122 (1965)
Kuffler, S. W., Nicholls, J. G.: The physiology of neuroglial cells. Ergebn. Physiol57, 1–90 (1966)
Li, C. L., Cullen, C., Jasper, H. H.: Microelectrode studies of electrical activity of the cerebral cortex in the cat. J. Physiol. (Lond.)121, 117–140 (1953)
Lipscomb, W. T., Boyarski, L. L.: Neurophysiological investigation of medullary chemosensitive areas of respiration. Respir. Physiol.16, 362–376 (1972)
Loeschcke, H. H.: Respiratory chemosensitivity in the medulla oblongata. Acta neurobiol. exp.33, 97–112 (1973)
Loeschcke, H. H.: Central nervous chemoreceptors. In: Respiration physiology (J. G. Widdicombe, ed.), pp. 167–196, London: Butterworth 1974
Loeschcke, H. H., de Lattre, I., Schläfke, M. E., Trouth, C. O.: Effects on respiration and circulation of electrically stimulating the ventral surface of the medulla oblongata. Respir. Physiol.10, 184–197 (1970)
Mitchell, R. A., Loeschcke, H. H., Massion, W. H., Severinghaus, J. W.: Respiratory response mediated through superficial chemosensitive areas on the medulla. J. appl. Physiol.18, 523–533 (1963)
Petrovicky, P.: Über die Glia marginalis und oberflächliche Nervenzellen im Hirnstamm der Katze. Z. Anat. Entwickl.-Gesch.127, 221–231 (1968)
Phillips, C. G.: Intracellular records from Betz cells in the cat. Quart. J. exp. Physiol.41, 58–69 (1956)
Salmoiraghi, G. C., Burns, B. D.: Localization and patterns of discharge of respiratory neurons in brain-stem of cat. J. Neurophysiol.23, 2–13 (1960)
Schläfke, M. E., Loeschcke, H. H.: Lokalisation eines an der Regulation von Atmung und Kreislauf beteiligten Gebietes an der ventralen Oberfläche der Medulla oblongata durch Kälteblockade. Pflügers Arch.297, 201–220 (1967)
Schläfke, M. E., See, W. R., Herker, A.: Response of neurons in the ventral medullary surface to alteration of H+ ion concentration in the cerebrospinal fluid. Pflügers Arch.359, (Suppl.), R 48 (1975a)
Schläfke, M. E., See, W. R., Loeschcke, H. H.: Ventilatory response to alterations of H+ ion concentration in small area of the ventral medullary surface. Respir. Physiol.10, 198–212 (1970)
Schläfke, M. E., Kille, J. F., Folgering, H., Herker, A., See, W. R.: Breathing without chemosensitivity. In: Central rhythmic and regulation (W. Umbach and H. P. Koepchen, eds.), pp. 97–104. Stuttgart: Hippokrates Verlag 1974
Schläfke, M. E., Pokorski, M., See, W. R., Prill, R. K., Loeschcke, H. H.: Chemosensitive neurons on the ventral medullary surface. Bull. Physiopath. Resp.11, 277–284 (1975b)
Speckmann, E. J., Caspers, H.: Verschiebung des corticalen Bestandspotentials bei Veränderungen der Ventilationsgröße. Pflügers Arch.310, 235–250 (1969)
Speckmann, E. J., Caspers, H., Sokolov, W.: Aktivitätsänderungen spinaler Neurone während und nach einer Asphyxie. Pflügers Arch.319, 122–138 (1970)
Tasaki, I., Polley, E. H., Orrego, F.: Action potential from individual elements in cat geniculate and striate cortex. J. Neurophysiol.17, 454–474 (1954)
Trouth, C. O., Loeschcke, H. H., Berndt, J.: Histological structures in the chemosensitive regions on the ventral surface of the cat's medulla oblongata. Pflügers Arch.339, 171–183 (1973)
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Fukuda, Y., Honda, Y. pH sensitivity of cells located at the ventrolateral surface of the cat medulla oblongata in vitro. Pflugers Arch. 364, 243–247 (1976). https://doi.org/10.1007/BF00581762
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DOI: https://doi.org/10.1007/BF00581762