Abstract
The influence of elevated levels of oleate on insulin-stimulated 3-0-methylglucose transport was assessed in vitro, in isolated skeletal muscle obtained from patients with type 2 (non-insulin-dependent) diabetes mellitus (n=7) and control subjects (n=8). An increase in oleate levels from 0.3 to 1.0 mmol/l induced a 3.7-fold increase in the rate of oleate oxidation (P<0.001) in skeletal muscle from control subjects. However, the rate of insulinstimulated 3-0-methylglucose transport was not altered in isolated skeletal muscle from the control subjects or the type 2 diabetic patients following exposure to 1.0 mmol/l oleate. This observation indicates that elevation of nonesterified fatty acids to a high physiological level has no inhibitory effect on glucose transport.
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References
Randle PJ, Garland PB, Hales CN, Newsholme EA, The glucose fatty-acid cycle: its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet I:785–789, 1963
Randle PJ, Newsholme EA, Garland PB, Regulation of glucose uptake by muscle. 8. Effects of fatty acids, ketone bodies, pyruvate, and of alloxan diabetes and starvation, on the uptake and metabolic fate of glucose in rat heart and diaphragm muscles. Biochem J 93:652–665, 1964
Nuutila P, Koivisto VA, Knnuti J, Ruotsalainen U, Teräs M, Haaparanta M, Bergman J, Solin O, Voipio-Pulkki L-M, Wegelius U, Yki-Järvinen H, Glucose-free fatty acid cycle operates in human heart and skeletal muscle in vivo. J Clin Invest 89:1767–1774, 1992
Argyraki M, Wright PD, Venables CW, Proud G, Taylor R, In vitro study of human skeletal muscle strips: effect of nonesterified fatty acid supply on glucose storage. Metabolism 38:1183–1187, 1989
Groop LC, Bonadonna RC, DelPrato S, Ratheiser K, Zyck K, Ferrannini E, DeFronzo RA, Glucose and free fatty acid metabolism in non-insulin-dependent diabetes mellitus. J Clin Invest 84:205–213, 1989
Dohm GL, Tapscott EB, Pories WJ, Dabbs DJ, Flickinger EG, Meelheim D, Fushiki T, Atkinson SM, Elton CW, Caro JF, An in vitro human muscle preparation suitable for metabolic studies. J Clin Invest 82:486–494, 1988
Andréasson K, Galuska D, Thörne A, Sonnenfeld T, Wallberg-Henriksson H, Decreased insulin-stimulated 3-0-methylglucose transport in in vitro incubated muscle strips from type II diabetic subjects. Acta Physiol Scand 142:255–260, 1991
Zierath JR, Galuska D, Engström Å, Johanson KH, Betsholtz C, Westermark P, Wallberg-Henriksson H, Human islet amyloid polypeptide at pharmacological levels inhibits insulin and phorbol ester-stimulated glucose transport in in vitro incubated human muscle strips. Diabetologia 35:26–31, 1992
Johansson U, Eriksson LS, Galuska D, Zierath JR, Wallberg-Henriksson H, Insulin action on glucose transport in isolated skeletal muscle from patients with liver cirrhosis. Scand Gastroenterol 29:71–76, 1994
Bergman RN, Ider YZ, Bowden CR, Cobelli C, Quantitative estimation of insulin sensitivity. Am J Physiol 236:E667–E677 1979
Wallberg-Henriksson H, Nie Z, Henriksson J, Reversibility of decreased insulin-stimulated glucose transport capacity in diabetic muscle with in vitro incubation: insulin is not required. J Biol Chem 262:7665–7671, 1987
Young DA, Ho RS, Bell PA, Cohan DK, McIntosh RN, Nadelson J, Foley JE, Inhibition of hepatic glucose production by SDZ 5141. Diabetes 39:1408–1413, 1990
Zierath JR, Galuska D, Nolte L, Thörne A, Smedegaard Kristensen J, Wallberg-Henriksson H, Effects of glycaemia on glucose transport in isolated skeletal muscle from patients with type II (non-insulin-dependent) diabetes mellitus: In vitro reversal of muscular insulin resistance. Diabetologia 37:270–277, 1994
Ferrannini E, Barrett EJ, Bevilacqua S, DeFronzo RA, Effect of fatty acids on glucose production and utilization in man. J Clin Invest 72:1737–1747, 1983
Wallberg-Henriksson H, Glucose transport into skeletal muscle. Influence of contractile activity, insulin, catechlamines and diabetes mellitus. Acta Physiol Scand 131:[Suppl 564]:1–80, 1987
Falholt K, Jensen I, Lindkæe Jensen S, Mortensen H, Volund Aa, Heding LG, Noerskov Petersen P, Falholt W, Carbohydrate and lipid metabolism of skeletal muscle in type 2 diabetic patients. Diabetic Med 5:27–31, 1988
Nolte LA, Galuska D, Martin IK, Zierath JR, Wallberg-Henriksson H, Elevated free fatty acid levels inhibit glucose phosphorylation in slow-twitch rat skeletal muscle. Acta Physiol Scand 151:51–59, 1994
Hardy RW, Ladenson JH, Henriksen EJ, Holloszy JO, McDonald JM, Palmitate stimulates glucose transport in rat adipocytes by a mechanism involving translocation of the insulin, sensitive glucose transporter (GLUT4). Biochem Biophys Res Commun 177:343–349, 1991
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Galuska, D., Nolte, L., Wahlström, E. et al. Effects of non-esterified fatty acids on insulin-stimulated glucose transport in isolated skeletal muscle from patients with type 2 (non-insulin-dependent) diabetes mellitus. Acta Diabetol 31, 169–172 (1994). https://doi.org/10.1007/BF00570374
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DOI: https://doi.org/10.1007/BF00570374