Summary
Using a set of overlapping deletion mutants in the tetracycline-resistance transposon Tn10, it has been established that certain regions of the Tn10 genome exert a powerful inhibition on translocation of an intact Tn10 element into the bacterial genome. Such inhibition is strongly temperature dependent: at 37° C translocation is inhibited by at least a factor of 100; no inhibition of translocation is detected at 30° C.
This is a preview of subscription content, log in via an institution to check access.
References
Beck CF (1979) A genetic approach to the analysis of transposons. Proc Natl Acad Sci USA 76:2376–2380
Chou J, Casadaban MJ, Lemaux P, Cohen SN (1979) Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. Proc Natl Acad Sci USA 76:4020–4024
Franklin TJ (1967) Resistance of Escherichia coli to tetracyclines. Changes in permeability to tetracyclines in Escherichia coli bearing transferable resistance factors. Biochem J 105:371–378
Heffron F, Bedinger P, Champoux JJ, Falkow S (1977) Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci USA 74:702–706
Heffron F, So M, McCarthy BJ (1978) In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites. Proc Natl Acad Sci USA 75:6012–6016
Heffron F, McCarthy BJ, Ohtsubo H, Ohtsubo E (1979) DNA sequence analysis of the transposon Tn3: Three genes and three sites involved in transposition of Tn3. Cell 18:1153–1163
Kleckner N, Chan RK, Tye B-K, Botstein D (1975) Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol 97:561–575
Kleckner N, Roth J, Botstein D (1977) Genetic engineering in vivo using translocatable drug-resistance elements. New Methods in Bacterial Genetics. J Mol Biol 116:125–159
Kretschmer PJ, Cohen SN (1979) Effect of temperature on translocation frequency of the Tn3 element. J Bacteriol 139:515–519
Müller-Hill B, Kania J (1974) Lac repressor can be fused to β-galactosidase. Nature (London) 249:561–563
Robinson MK, Bennett PM, Richmond MH (1977) Inhibition of TnA translocation by TnA. J Bacteriol 129:407–414
Robinson MK, Bennett PM, Grinsted T, Richmond MH (1978) The stable carriage of two TnA units on a single replicon. Mol Gen Genet 160:339–346
Vogel HJ, Bonner DM (1956) Acetylornithinase of Escherichia coli: Partial purification and some properties. J Biol Chem 218:97–106
Author information
Authors and Affiliations
Additional information
Communicated by M.M. Green
Rights and permissions
About this article
Cite this article
Beck, C.F., Moyed, H. & Ingraham, J.L. The tetracycline-resistance transposon Tn10 inhibits translocation of Tn10. Molec. Gen. Genet. 179, 453–455 (1980). https://doi.org/10.1007/BF00425476
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425476