Abstract
Single cod [Gadus Morhua (L.)] and haddock [Melanogrammus aeglefinus (L.)] eggs from the first day and the eighth day after fertilization were acid hydrolised. Fatty acids were liberated and derivatized to methyl esters in a one-step reaction. The non-polar hexane extract was analysed by High Resolution Gas Chromatography (HRGC). Selected peaks, occuring in both types of samples and in all chromatograms, were used to create a matrix for multivariate analysis. HRGC and Mass Spectrometry have been used to identify the fatty acids. The approach is shown to be useful in distinguishing between cod and haddock eggs at both stages and information regarding changes in the fatty acid distributions are obtained. Implications on future possibilities within chemosystematics are discussed.
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Albano, C., W. J. Dunn III, U. Edlund, E. Johansson, B. Norden, M. Sjøstrøm and S. Wold: Four levels of pattern recognition. Anal. chim. Acta. 103, 429–443 (1978)
Albano, C., S. Wold, G. Blomquist, D. Coomans, W. J. Dunn III, U. Edlund, B. Eliasson, S. Helleberg, E. Johansson, B. Norden, M. Sjøstrøm, B. Søderstrøm and H. Wold. Pattern recognition by the method of disjoint principal component models (SIMCA), pp 183–218. Proc. Symp. applied statistics, NEUCC, DTH' Lyngby, NOVO Fonden, Denmark 1981
Bergan, T., P. A. D. Grimont and F. Grimont: Fatty acids of Serratia determined by gas chromatography. Curr. Microbiol. 8, 7–11 (1983)
Bisani, M. L., D. Faraone, S. Clementi, K. H. Esbensen and S. Wold: Principal component and partial least squares analysis of the geochemistry of volcanic rocks from the Aeolian archipelago. Anal. chim. Acta. 150, 129–144 (1983)
Bjorvatn, B., V. Lund, B.-E. Kristiansen, L. Korsnes, O. Spanne and B. Lundquist: Applications of restriction endonuclease fingerprinting of chromosomal DNA of Neisseria meningitidis. J. clin. Microbiol. 19, 763–765 (1984)
Bobbie, R. J. and D. C. White: Characterization of benthic microbial community structure by high-resolution gas chromatography of fatty acid methyl esters. Appl. envir. Microbiol. 1212–1222 (1980)
Christie, O. H. J. and S. Wold: The influence of skewed measurement distributions commonly found in geochemistry. Anal. Lett. 12 (A9), 979–993 (1979)
Clark, A., N. Doherty, A. L. DeVries and J. T. Eastman: Lipid content and composition of three species of Antarctic fish in relation to buoyancy. Pol. Biol. 3, 77–83 (1984)
Dahl, E., D. S. Danielssen, E. Moksness and P. Solemdal: The propagation of cod (Gadus morhua L.), 895 pp. Parts I and II. An International symposium, Arendal, Norway. 14–17 June, 1983. 1984
Derde, M. P. and D. L. Massart: Extraction of information from large data sets by pattern recognition. Fresenius Z. anal. Chem. 313, 484–495 (1982)
Dunn III, W. J., S. Wold, U. Edlund, S. Hellberg and J. Gasteiger: Multivariate structure-activity relationships between data from a battery of biological tests and an ensemble of chemical descriptors: the PLS method. Quant. Struct.-Act. Relationships 3, 131–137 (1984)
Federle, T. W., R. J. Livingston, D. A. Meeter and D. C. White: Modifications of estuarine sedimentary microbiota by exclusion of epibenthic predators. J. exp. mar. Biol. Ecol. 73, 81–94 (1983)
Henderson, R. J., J. R. Sargent and C. C. E. Hopkins: Changes in the content and fatty acid composition of lipid in an isolated population of the capelin Mallotus villosus during sexual maturation and spawning. Mar. Biol. 78, 255–263 (1984)
Jangaard, P. M., R. G. Ackman and J. C. Sipos: Seasonal changes in fatty acid composition of cod liver, flesh, roe and milt lipids. J. Fish. Res. Bd Can. 24, 613–627 (1967)
Jantzen, E.: Gas chromatography of bacterial whole cell methanolysates. A chemotaxonomic study with special reference to Neisseriaceae. Ph.D. thesis. University of Oslo, Norway 1976
Johansson, E., S. Wold and K. Sjødin: Minimizing the effect of closure on analytical data. Anal. Chem. 56, 1685–1688 (1984)
Johnson, A. G., F. M. Utter and H. O. Hodgkins: Study of the feasability of immunochemical methods for identification of pleuronectid eggs. J. Cons. int. Explor. Mer. 36, 158–161 (1975)
Knutsen, H., E. Moksness and N. B. Vogt: Distinguishing between I day old cod (Gadus morhua (L.)) and haddock (Melanogrammus aeglefinus (L.)) eggs using gas chromatography and SIMCA pattern recognition. J. Fish. Res. Bd Can. 42, 1823–1826 (1985)
Knutsen, H. and N. B. Vogt: A supplementary approach to identifying feeding patterns of juvenile lobsters (stage IV) using chemical analysis and pattern recognition by the method of SIMCA. Parts I and II. J. exp. mar. Biol. Ecol. 89, 109–131 (1985)
Koslow, J. A., S. Brault, J. Dugas, R. O. Fournier and P. Hughes: Condition of larval cod (Gadus morhua) off southwest Nova Scotia in 1983 in relation to plankton abundance and temperature. Mar. Biol. 86, 113–121 (1985)
Martens, H. and H. Russwurm (Eds.): Food research and data analysis, 535 pp. London: Applied Science Publ. 1983
Massart, D. L. and L. Kaufman: The interpretation of analytical chemical data by the use of cluster analysis. Chemical Analysis, vol. 65, 237 pp. New York: John Wiley & Sons 1983
Matta, J. F. and H. G. Marshall: A multivariate analysis of phytoplankton populations in the gulf of Maine, 14 pp. ICES paper. Biol. Oceanogr. Comm. C.M. 1983/E:60, 1983
Moksness, E. and J. Riis-Vestergaard: Spawning of haddock (Melanogrammus aeglefinus) in captivity. Flødevigen Rapportseries 2, 1–9 (1982)
Mork, J., P. Solemdal and G. Sundnes: Identification of marine fish eggs: A biochemical approach. Can. J. Fish. aquat. Sci 40, 361–369 (1983)
Mork, J., C. Reuterwall, N. Ryman and G. Ståhl: Genetic variation in Atlantic cod (Gadus morhua L.): a quantitative estimate from a Norwegian coastal population. Hereditas 96, 55–61 (1982)
Oshima, T., S. Wada and C. Koizuma: Estimation of possible fatty acid combinations in phosphatidylcholine and phosphatidyl-ethanolamine of cod. Bull. Jap. Soc. Sci. Fish. 49, 123–130 (1983)
Rezanka, T., J. Vokoun, J. Slavicek and M. Podojil: Determination of fatty acids in algae by capillary gas chromatographymass spectrometry. J. Chrom. 268, 71–78 (1983)
Shaw, N.: Lipid composition as the guide to the classification of bacteria. In: Advances in applied microbiology, pp 63–105. Ed. by D. Perlman. New York: Academic Press 1974
Smeyers-Verbeke, J., J. C. den Hartog, W. H. Dekker, D. Coomans, L. Buydens and D. L. Massart: The use of principal component analysis for the investigation of an organic air pollutants data set. Atmos. Envir. 18, 2471–2478 (1984)
Sneath, P. H. A. and R. R. Sokal: Numerical taxonomy. The principles and practice of numerical classification, 573 pp. San Francisco: W. H. Freeman and Company 1973
Sundberg, P.: Multivariate analysis in marine pollution studies. Mar. Poll. Bull. 14, 208–209 (1983)
Thielemans, A. and D. L. Massart: The use of principal component analysis as a display method in the interpretation of analytical chemical, biochemical, environmental and epidemiological data. Chimia 39, 236–242 (1985)
Tocher, D. R. and J. R. Sargent: Analyses of lipids and fatty acids in ripe roes of some northwest European marine fish. Lipids 19, 492–499 (1984)
Tocher, D. R., A. J. Fraser, J. R. Sargent and J. C. Gamble: Fatty acid composition of phospholipids and neutral lipids during embryonic and early larval development in atlantic herring (Clupea harengus (L.)). Lipid 20, 69–74 (1985a)
Tocher, D. R., A. J. Fraser, J. R. Sargent and J. C. Gamble: Lipid class composition during embryonic and early larval development in Atlantic herring (Clupea harengus (L.)). Lipids 20, 84–89 (1985b)
Vetter, R. D., R. E. Hodson and C. Arnold: Energy metabolism in rapidly developing marine fish egg, the red drum (Sciaenops ocellata). Can. J. Fish. aquat. Sci. 40, 627–634 (1983)
Vogt, N. B. and H. Knutsen: SIMCA pattern recognition classification of five infauna taxonomic groups using non-polar compounds analysed by high resolution gas chromatography. Mar. Ecol. Prog. Ser. 26, 145–156 (1985)
Wold, S.: Pattern recognition by means of disjoint principal component models. Pattern Recognition 8, 127–139 (1976)
Wold, S.: Cross validatory estimation of the number of components in factor and principal component models. Technometrics 20, 175–177 (1978)
Wold, S. and M. Sjøstrøm: SIMCA: a method for analysing chemical data in terms of similarity and analogy. ACS symposium series, no. 52. Chemometrics: theory and application, pp 243–282. Ed. by B. Kowalski, 1977
Wold, S., C. Albano, W. J. Dunn III, U. Edlund, K. Esbensen, P. Geladi, S. Hellberg, E. Johansson, W. Lindberg and M. Sjøstrøm: Multivariate data analysis in chemistry. In: Proc. NATO Adv. Study Institute on Chemometrics. Cosenza, Italy, 484 pp. Ed. by B. R. Kowalski. Dordrecht, Holland: Reidel Publ. Co. 1984a
Wold, S., C. Albano, W. J. Dunn III, K. Esbensen, S. Hellberg, E. Johansson and M. Sjøstrøm: Multivariate analytical chemical data evaluation using SIMCA and MACUP. In: Modern trends in analytical chemistry, pp 157–188. Ed. by E. Punger and G. E. Veress. Budapest: Akademiai Kiado 1984b
Wold, S., G. Blomquist, E. Johansson and B. Søderstrøm: Classification of fungi by means of pyrolysis-gas chromatography and pattern recognition. J. Chromatogr. 173, 19–32 (1979)
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Communicated by T. Fenchel, Aarhus
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Vogt, N.B., Moksness, E., Sporstøl, S.P. et al. SIMCA principal component analysis of fatty acid patterns in Day-1 and Day-8 cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) eggs. Marine Biology 92, 173–182 (1986). https://doi.org/10.1007/BF00392834
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DOI: https://doi.org/10.1007/BF00392834