Summary
A replication map for strain C of Escherichia coli was constructed using the three different attachment sites, location I, II and III, of prophage P2, and the one of prophage λ in a marker-frequency analysis. Marker-frequencies for the four prophage locations were estimated by hybridization of radioactive phage DNA with DNA extracted from exponentially growing lysogenic bacteria, and immobilized on nitrocellulose membrane filters.
The results obtained were interpreted according to a model of bidirectional symmetric chromosome replication, with the replication points starting in the left upper quadrant of the genetic map (at 86±1 minutes on a 101 minute map), and proceeding simultaneously in both directions to a terminus in the right lower quadrant. The results are inconsistent with a unidirectional mode of chromosome replication.
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References
Abe, M., Tomizawa, J.: Replication of the Escherichia coli K-12 chromosome. Proc. nat. Acad. Sci. (Wash.) 58, 1911–1918 (1967)
Berns, K. I., Thomas, C. A., Jr.: Isolation of high molecular weight DNA from Hemophilus influenzae. J. molec. Biol. 11, 476–490 (1965).
Bertani, L. E.: The effect of ultraviolet light on the establishment of lysogeny. Virology 7, 92–111 (1959).
Bertani, L. E.: Stabilization of P2 tandem double lysogens by int mutations in the prophage. Virology 46, 426–436 (1971).
Bertani, L. E., Bertani, G.: Preparation and characterization of temperate, non-inducible bacteriophage P2 (host: Escherichia coli). J. gen. Virol. 6, 201–212 (1970).
Bird, R. E., Louarn, J., Martuscelli, J., Caro, L. G.: The origin and sequence of chromosome replication in Escherichia coli. J. molec. Biol. 70, 549–566 (1972).
Britten, R. J., Kohne, D. E.: Repeated sequences in DNA. Science 161, 529–540 (1968).
Burgi, E., Hershey, A. D.: Sedimentation rate as a measure of molecular weight of DNA. Biophys. J. 3, 309–321 (1963).
Cairns, J.: The bacterial chromosome and its manner of replication as seen by autoradiography. J. molec. Biol. 6, 208–213 (1963).
Cairns, J.: The chromosome of Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 28, 43–45 (1963).
Calendar, R., Lindahl, G.: Attachment of prophage P2: Gene order at different host chromosomal sites. Virology 39, 867–881 (1969).
Caro, L. G., Berg, C. M.: Chromosome replication in some strains of Escherichia coli K-12. Cold Spr. Harb. Symp. quant. Biol. 33, 559–573 (1968).
Cerdá-Olmedo, E., Hanawalt, P. C., Guerola, N.: Mutagenesis of the replication point by nitrosoguanidine. Map and pattern of replication of the Escherichia coli chromosome. J. molec. Biol. 33, 705–719 (1968).
Clark, D. J., Maaløe, O.: DNA replication and the division cycle in Escherichia coli. J. molec. Biol. 23, 99–112 (1967).
Cooper, S., Helmstetter, C. E.: Chromosome replication and the division cycle of Escherichia coli B/r. J. molec. Biol. 31, 519–540 (1968).
Denhardt, D. T.: A membrane-filter technique for the detection of complementary DNA. Biochem. biophys. Res. Commun. 23, 641–646 (1966).
Helmstetter, C. E., Cooper S.: DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J. molec. Biol. 31, 507–518 (1968).
Inman, R. B., Bertani, G.: Heat denaturation of P2 bacteriophage DNA: Compositional heterogeneity. J. molec. Biol. 44, 533–549 (1969).
Lark, K. G.: Regulation of chromosome replication and segregation in bacteria. Bact. Rev. 30, 3–32 (1966).
Lark, K. G., Repko, T., Hoffman, E.: The effect of amino acid deprivation on subsequent deoxyribonucleic acid replication. Biochim. biophys. Acta (Amst.) 73, 9–24 (1963).
Lindahl, G., Sironi, G., Bialy, H., Calendar, R.: Bacteriophage Lambda: Abortive infection of bacteria lysogenic for phage P2. Proc. nat. Acad. Sci (Wash.) 66, 587–594 (1970).
Maaløe, O., Hanawalt, P. C.: Thymidine deficiency and the normal DNA replication cycle. I. J. molec. Biol. 3, 144–155 (1961).
Maaløe, O., Kjeldgaard, P. C.: Control of macromolecular synthesis: A study of DNA, RNA and protein synthesis in bacteria. New York: Benjamin 1966.
Mandell, J. D., Hershey, A. D.: A fractionating column for analysis of nucleic acids. Analyt. Biochem. 1, 66–77 (1960).
Marmur, J.: A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. molec. Biol. 3, 208–218 (1961).
Masters, M., Broda, P.: Evidence for the bidirectional replication of the Escherichia coli chromosome. Nature (Lond.) New Biol. 232, 137–140 (1971).
Meselson, M., Stahl, F.W.: The replication of DNA in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 44, 671–682 (1958).
Nagata, T.: The molecular synchrony and sequential replication of DNA in Escherichia coli. Genetics 49, 551–559 (1963).
Nishioka, Y., Eisenstark A.: Sequence of genes replicated in Salmonella typhimurium as examined by transduction techniques. J. Bact. 102, 320–333 (1970).
Sasaki, I., Bertani, G.: Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J. gen. Microbiol. 40, 365–376 (1965).
Schnös, M., Inman, R. B.: Position of branch points in replicating λ DNA. J. molec. Biol. 51, 61–73 (1970).
Sueoka, N., Yoshikawa, H.: The chromosome of B. subtilis. I. Theory of marker frequency analysis. Genetics 52, 747–757 (1965).
Taylor, A. L., Trotter, C. D.: Revised linkage map of Escherichia coli. Bact. Rev. 31, 332–353 (1967).
Wiman, M., Bertani, G., Kelly, B., Sasaki, I.: Genetic map of Escherichia coli strain C. Molec. gen. Genet. 107, 1–31 (1970).
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Communicated by P. Starlinger
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Jonasson, J. Evidence for bidirectional chromosome replication in Escherichia coli C based on marker-frequency analysis by DNA/DNA hybridization with P2 and λ prophages. Molec. gen. Genet. 120, 69–90 (1973). https://doi.org/10.1007/BF00332985
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DOI: https://doi.org/10.1007/BF00332985