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Nucleotide sequence of the glnA control region of Escherichia coli

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Summary

The RNA polymerase binding sites present along a DNA segment encompasing the glnA, glnL, and glnG genes have been identified in a hybrid plasmid carrying this chromosomal region of Escherichia coli. The DNA sequence was determined of an 817 base pair segment that contains the region coding for the first 42 amino acids of the NH2-terminal and of the glnA structural gene, as well as its regulatory region. Analysis of this nucleotide sequence revealed three probable RNA polymerase recognition sites, imperfect palindromes, inverted repeats, and direct repeated sequences.

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References

  • Bender RA, Magasanik B (1977) Regulatory mutations in the Klebsiella aerogenes structural gene for glutamine synthetase. J Bacteriol 132:100–105

    Google Scholar 

  • Covarrubias AA, Rocha M, Bolivar F, Bastarrachea F (1980a) Cloning and physical mapping of the glnA gene of Escherichia coli K-12. Gene 11:239–251

    Google Scholar 

  • Covarrubias AA, Sánchez-Pescador R, Osorio A, Bolivar F, Bastarrachea F (1980b) ColE1 hybrid plasmids containing the Escherichia coli genes involved in the biosynthesis of glutamate and glutamine. Plasmid 3:150–164

    Google Scholar 

  • Foor F, Reuveny Z, Magasanik B (1980) Regulation of the synthesis of glutamine synthetase by the PII protein in Klebsiella aerogenes. Proc Natl Acad Sci USA 77:2636–2640

    Google Scholar 

  • Gaillardin CM, Magasanik B (1978) Involvement of the product of the glnF gene in the autogenous regulation of glutamine synthetase-formation in Klebsiella aerogenes. J Bacteriol 133:1329–1338

    Google Scholar 

  • Ginsburg A, Stadtman ER (1973) Regulation of glutamine synthetase in Escherichia coli. In: Prusiner S, Stadtman ER (eds) The enzymes of glutamine metabolism. Academic Press, New York, pp 9–43

    Google Scholar 

  • Higgins CF, Ames FG (1982) Regulatory regions of two transport operons under nitrogen control: Nucleotide sequences. Proc Natl Acad Sci USA 79:1083–1087

    Google Scholar 

  • Kingdon SH, Noyes C, Lahiri A, Heinrikson RL (1972) Primary structure of Escherichia coli glutamine synthetase. J Biol Chem 247:7923–7926

    Google Scholar 

  • Kustu S, Burton D, García E, McCarter L, McFarland N (1979) Nitrogen control in Salmonella: Regulation by the glnR and glnF gene products. Proc Natl Acad Sci USA 76:4576–4580

    Google Scholar 

  • Messing J, Crea R, Seeburg PH (1981) A system for shotgun DNA sequencing. Nucl Acids Res 9:309–321

    Google Scholar 

  • McFarland N, McCarter L, Artz S, Kustu S (1981) Nitrogen regulatory locus “glnR” of enteric bacteria is composed of cistrons ntrB and ntrC: identification of their products. Proc Natl Acad Sci USA 78:2135–2139

    Google Scholar 

  • Pahel G, Tyler B (1979) A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli. Proc Natl Acad Sci USA 76:4544–4548

    Google Scholar 

  • Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Shine J, Dalgarno L (1974) The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA; complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346

    Google Scholar 

  • Stuber D, Bujard H (1981) Organization of transcriptional signals in plasmids pBR322 and pACYC 184. Proc Natl Acad Sci USA 78:167–171

    Google Scholar 

  • Tyler B (1978) Regulation of the assimilation of nitrogen compounds. Annu Rev Biochem 47:1127–1162

    Google Scholar 

  • West RW, Rodríguez RL (1980) Construction and characterization of E. coli promoter—probe plasmid vectors II. RNA polymerase binding studies on antibiotic—resistance promoters. Gene 9:175–193

    Google Scholar 

  • Woolfolk CA, Shapiro BM, Stadtman ER (1966) Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase of Escherichia coli. Arch Biochem Biophys 116:177–192

    Google Scholar 

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Communicated by G. O'Donovan

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Covarrubias, A.A., Bastarrachea, F. Nucleotide sequence of the glnA control region of Escherichia coli . Mol Gen Genet 190, 171–175 (1983). https://doi.org/10.1007/BF00330342

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