Abstract
Hybridising certain AT-rich satellite complementary RNAs (cRNAs) to their homologous chromosomal DNA sequences at different temperatures of incubation results in a different dispersion of autoradiographic label throughout the karyotypes. The temperature at which most label, or eRNA-DNA hybrid formation, exists corresponds to the optimal rate temperature for the hybridisation of these same satellite cRNA-DNA hybrids as determined by RNA excess filter hybridisation. It is likely that the in situ hybridisation results can therefore be explained by the fact that there is a similar temperature-dependence on the rate of hybrid formation for both in situ and RNA excess hybridisation. This should have important implications for the designing of in situ hybridisation experiments in general.
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Moar, M.H., Purdom, I.F. & Jones, K.W. Influence of temperature on the detectibility and chromosomal distribution of specific DNA sequences by in situ hybridisation. Chromosoma 53, 345–359 (1975). https://doi.org/10.1007/BF00294082
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DOI: https://doi.org/10.1007/BF00294082