Summary
mRNA of the galactose operon of E. coli was measured in wildtype E. coli and in gal operon amber and insertion mutants. The mRNA coded by the distal half of the operon is reduced in the mutants. This reduction is more pronounced in the insertion mutants than in the amber mutants. It was compared with the polar effects of the mutations on the enzymes of the operon.
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Adesnik, M., Levinthal, C.: The synthesis and degradation of lactose operon messenger RNA in E. coli. Cold Spr. Harb. Symp. quant. Biol. 35, 451 (1970).
Altman, S.: Isolation of tyrosine tRNA precursor molecules. Nature (Lond.) New Biol. 229, 19 (1971).
Bøvre, K., Szybalski, W.: Multistep DNA-RNA hybridization techniques. Methods in enzymology, vol. 21, p. 350 (ed.: L. Grossman, and K. Moldave). New York and London: Academic Press 1971.
Buttin, G.: Mechanismes régulateur dans la biosynthèse des enzymes du métabolisme du galactose chez Escherichia coli K12. I. La biosynthèse induite de la galactokinase et l'induction simultanée de la sequence enzymatique. J. molec. Biol. 7, 164 (1963).
Contesse, G., Crépin, M., Gros, F.: Transcription of the lactose operon in E. coli. In: The lactose operon (ed.: J. Beckwith and D. Zipser), p. 111. Cold Spring Harbor 1970.
Feiss, M., Adhya, S., Court, D. L.: Isolation of plaque-forming, galactose-transducing strains of phage lambda. Genetics 71, 189 (1972).
Fiandt, M., Szybalski, W., Malamy, M. H.: Polar mutations in Lac, gal and phage λ consist of a few DNA sequences inserted with either orientation. Molec. gen. Genet. 119, 223 (1972).
Goldberg, A. R., Howe, M.: New mutations in the S cistron of bacteriophage λ affecting host cell lysis. Virology 38, 200 (1969).
Gosden, J. R., Irving, M. I., Bishop, J. O.: Galactose-specific messenger ribonucleic acid contents in Escherichia coli. Biochem. J. 121, 109 (1971).
Guha, A., Tabaczyński, M., Sszybalski, W.: Orientation of transcription for the galactose operon as determined by hybridization of gal-mRNA with the separated DNA strands of coliphage λdg. J. molec. Biol. 35, 207 (1968).
Hill, C. W., Echols, H.: Properties of a mutant blocked in inducibility of messenger RNA for the galactose operon of Escherichia coli. J. molec. Biol. 19, 38 (1966).
Hirsch, H.-J., Saedler, H., Starlinger, P.: Insertion mutations in the control region of the gal Operon of E. coli. II. Physical characterization of the mutations. Molec. gen. Genet. 115, 266 1972a.
Hirsch, H.-J., Starlinger, P., Brachet, P.: Two kinds of insertions in bacterial genes. Molec. gen. Genet. 119, 191 (1972b).
Jordan, E., Saedler, H., Starlinger, P.: Strong-polar mutations in the transferase gene of the galactose operon in E. coli. Molec. gen. Genet. 100, 296 (1967).
Jordan, E., Yarmolinsky, M., Kalckar, H. M.: Control of inducibility of enzymes of the galactose sequence in E. coli. Proc. nat. Acad. Sci. (Wash.) 48, 32 (1962).
Kemper, B.: Increased recombinant frequencies with an amber mutation in the gal operon of E. coli. Molec. gen. Genet. 107, 107 (1970).
Mackie, G., Wilson, D. B.: Polarity and transcription in the galactose operon of E. coli. Biochem. biophys. Res. Commun. 48, 226 (1972).
Malamy, M. H., Fiandt, M., Szybalski, W.: Electron microscopy of polor insertions in the lac operon of Escherichia coli. Molec. gen. Genet. 119, 207 (1972).
Michaelis, G., Saedler, H., Venkov, P., Starlinger, P.: Two insertions in the galactose operon having different sizes but homologous DNA sequences. Molec. gen. Genet. 104, 371 (1969).
Pfeifer, D., Oellermann, R.: Mapping of gal- mutants by transducing λdg carrying deletions of the gal region. Molec. gen. Genet. 99, 248 (1967).
Saedler, H., Besemer, J., Kemper, B., Rosenwirth, B., Starlinger, P.: Insertion mutations in the control region of the gal operon of E. coli. I. Biological characterization of the mutations. Molec. gen. Genet. 115, 258 (1972).
Saedler, H., Starlinger, P.: 0° mutations in the galactose operon in E. coli. II. Physiological characterization. Molec. gen. Genet. 100, 190 (1967).
Shapiro, J. A.: The structure of the galactose operon in E. coli K-12. Ph. D. Thesis, University of Cambridge, England, 1967.
Starlinger, P., Saedler, H.: Insertion mutations in microorganisms. Biochemie 54, 177 (1972).
Szybalski, W., Kubinski, H., Hradecna, Z., Summers, W. C.: Analytical and preparative separation of the complementary DNA strands. Methods in enzymology, vol. 21, p. 386. (eds.: L. Grossman and K. Moldave). New York and London: Academic Press 1971.
Wetekam, W., Staack, K., Ehring, R.: DNA-dependent in vitro synthesis of enzymes of the galactose operon of Escherichia coli. Molec. gen. Genet. 112, 14 (1971).
Wilson, D. B., Hogness, D. S.: The enzymes of the galactose operon in Escherichia coli. IV. The frequencies of translation of the terminal cistrons in the operon. J. biol. Chem. 244, 2143 (1969).
Zabin, I., Fowler, A. V.: β-galactosidase and thiogalactoside transacelylase. In: The lactose operon (ed.: J. Beckwith and D. Zipser), p. 27. Cold Spring Harbor 1970.
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Communicated by E. Bautz
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Starlinger, P., Saedler, H., Rak, B. et al. mRNA distal to polar nonsense and insertion mutations in the gal operon of E. coli . Molec. Gen. Genet. 122, 279–286 (1973). https://doi.org/10.1007/BF00278603
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DOI: https://doi.org/10.1007/BF00278603