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A simple method for R banding of human chromosomes, showing a pH-dependent connection between R and G bands

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Summary

Incubation in an unbuffered solution of 1 M NaH2PO4 at 88°C (pH 4.0–4.5) causes R banding to appear on human chromosomes after subsequent staining in Giemsa dissolved in distilled water. G banding, results when the pH of the incubation medium is adjusted towards neutral.

Zusammenfassung

Bei Inkubation in einer ungepufferten 1 M NaH2PO4-Lösung bei 88°C (pH 4,0–4,5) und darauffolgender Färbung mit Giemsa in destilliertem Wasser erscheint ein R-Band-Muster auf menschlichen Chromosomen. Bei Änderung des pH des Inkubationsmediums in neutraler Richtung erscheint ein G-Band-Muster.

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References

  • Dutrillaux, B., Lejeune, J.: Sur une nouvelle technique d'analyse du caryotype humain. C.R. Acad. Sci. (Paris) 272, série D, 2638–2640 (1971)

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Authors and Affiliations

  1. Institute of Human Genetics, University of Aarhus, Aarhus, Denmark

    Jens Sehested

  2. Aarhus Kommunehospital, University of Aarhus, DK-8000, Aarhus C, Denmark

    Jens Sehested

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  1. Jens Sehested
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Sehested, J. A simple method for R banding of human chromosomes, showing a pH-dependent connection between R and G bands. Hum Genet 21, 55–58 (1974). https://doi.org/10.1007/BF00278565

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  • DOI: https://doi.org/10.1007/BF00278565

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