Summary
RSU2, a plasmid transmissible between strains of P. aeruginosa but not to Escherichia coli can be mobilized by R751. Conjugatants receive a single plasmid composed of DNA from both R751 and RSU2 which has the compatibility properties of a member of group P (like R751).
Study of this fusion plasmid suggests that the failure of RSU2 to transfer into enterobacteria is due to an inability to replicate in these bacteria. The fusion plasmid replicates using the genes of R751.
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References
Acar, J. F., Baudens, J. G., Chabbert, Y. A.: Clinical and laboratory evaluation of cephalexin in urinary-tract infections. Antimicrob. Agents and Chemother. 398-404 (1970)
Bachmann, B. J.: Pedigrees of some mutant strains of Escherichia coli K12. Bact. Rev. 36, 525–557 (1972)
Barth, P. T., Grinter, N. J.: Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulphonamides. J. Bact. 120, 618–630 (1974)
Bertani, G., Weigle, J. J.: Host controlled variation in bacterial viruses. J. Bact. 65, 113–121 (1953)
Bryan, L. E., Elzen, H. M. van den, Tseng, J. T.: Transferable drug resistance in Pseudomonas aeruginosa. Antimicrob. Agents and Chemother. 1, 22–29 (1972)
Bryan, L.E., Semaka, S. D., Elzen, H.M. van den, Kinnear, J. E., Whitehouse, R. L. S.: Characteristics of R931 and other Pseudomonas aeruginosa R factors. Antimicrob. Agents and Chemother. 3, 625–637 (1973)
Bryan, L. E., Shahrabadi, M. S., Elzen, H. M. van den,: Gentamicin resistance in Pseudomonas aeruginosa: R factor mediated resistance. Antimicrob. Agents and Chemother. 6, 191–199 (1974)
Burgi, E., Hershey, A. D.: Sedimentation rate as a measure of molecular weight of DNA. Biophys. J. 3, 309–321 (1963)
Clowes, R. C.: Molecular structure of bacterial plasmids. Bact. Rev. 36, 361–405 (1972)
Coetzee, J. N., Datta, N., Hedges, R. W.: R factors from Proteus rettgeri. J. gen. Microbiol. 72, 543–552 (1972)
Datta, N., Hedges, R. W.: R factors identified in Paris, some conferring gentamicin resistance, constitute a new compatibility group. Ann. Inst. Pasteur 123, 879–883 (1972)
Datta, N., Hedges, R. W.: An I-pilus determining R factor with anomalous compatibility properties, mobilizing a gentamicin-resistance plasmid. J. gen. Microbiol. 77, 11–17 (1973)
Datta, N., Hedges, R. W., Shaw, E. J., Sykes, R. B., Richmond, M. H.: Properties of an R factor from Pseudomonas aeruginosa. J. Bact. 108, 1244–1249 (1971)
Davis, J. E., Strauss, J. H., Sinsheimer, R. L.: Bacteriophage MS2: another RNA phage. Science 134, 1427 (1961)
Dennison, S.: Naturally occurring R factor, derepressed for Pilus Synthesis, belonging to the same compatibility group as the sex factor F of Escherichia coli K12. J. Bact. 109, 416–422 (1972)
Falkow, S., Guerry, P., Hedges, R. W., Datta, N.: Polynucleotide sequence relationships among plasmids of the I compatibility complex. J. gen. Microbiol. 85, 65–76 (1974)
Fullbrook, P. D., Elson, S. W., Slocombe, B.: R factor mediated β-lactamase in Pseudomonas aeruginosa. Nature (Lond.) 226, 1054–1056 (1970)
Grindley, N. D. F., Humphreys, G. O., Anderson, E. S.: Molecular studies of R factor compatibility groups. J. Bact. 115, 387–398 (1973)
Hedges, R. W.: R factors from swarming Proteus strains. J. gen. Microbiol. (in the press 1975)
Hedges, R. W., Datta, N.: R124, an fi + R factor of a new compatibility class. J. gen. Microbiol. 71, 403–405 (1972)
Hedges, R. W., Jacob, A. E.: Transposition of ampicillin resistance from RP4 to other replicons. Molec. gen. Genet. 132, 31–40 (1974)
Hedges, R. W., Jacob, A. E., Smith, J. T.: Properties of an R factor from Bordetella bronchiseptica. J. gen. Microbiol. 84, 199–204 (1974)
Iyobe, S., Hasuda, K., Fuse, A., Mitsuhashi, S.: Demonstration of R factors from Pseudomonas aeruginosa. Antimicrob. Agents and Chemother. 5, 547–552 (1974)
Jacob, A. E., Hobbs, S. J.: Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J. Bact. 117, 360–372 (1974)
Jacoby, G. A.: Properties of R plasmids determining gentamicin resistance by acetylation in Pseudomonas aeruginosa. Antimicrob. Agents and Chemother. 6, 239–252 (1974)
Jobanputra, R. S., Datta, N.: Trimethoprim resistance factors in enterobacteria from clinical specimens. J. med. Microbiol. 7, 169–177 (1974)
Kawakami, Y., Mikoshiba, F., Nagasaki, S., Matsumoto, H., Tazaki, T.: Prevalence of Pseudomonas aeruginosa strains possessing R factor in a hospital. J. Antibiot. (Tokyo) 25, 607–609 (1972)
Lawn, A. M., Meynell, E., Meynell, G. G., Datta, N.: Sex pili and the classification of sex factors in the Enterobacteriaceae. Nature (Lond.) 216, 343–346 (1967)
Mandel, M., Higa, A.: Calcium-dependent bacteriophage DNA infection. J. molec. Biol. 53, 159–162 (1970)
Olsen, R. H., Shipley, P.: Host range and properties of the Pseudomonas aeruginosa R factor R1822. J. Bact. 113, 772–780 (1973)
Sykes, R. B., Richmond, M. H.: Intergeneric transfer of a β-lactamase gene between Pseudomonas aeruginosa and Escherichia coli. Nature (Lond.) 226, 952–954 (1970)
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Communicated by W. Arber
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Hedges, R.W., Jacob, A.E. Mobilization of plasmid-borne drug resistance determinants for transfer from Pseudomonas aeruginosa to Escherichia coli . Molec. Gen. Genet. 140, 69–79 (1975). https://doi.org/10.1007/BF00268990
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DOI: https://doi.org/10.1007/BF00268990