Summary
The inactivation kinetics of two different Lambda phage repressors in lysogenic complexes of an E. coli strain temperature sensitive in DNA synthesis is investigated. From the experimental data obtained it is inferred that
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1.
after a stop of DNA synthesis effector molecules are accumulated which give rise to the inactivation of phage repressors directly,
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2.
the accumulation kinetics of the effector molecules after a stop of DNA synthesis is similar to that kinetics which must be expected from accumulation of DNA precursors.
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3.
The inactivation of λ+ and λind 8repressor molecules begins 20 min and 5 min, respectively, after the stop of host DNA synthesis.
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4.
The effector concentration is rapidly diminished after restoration of DNA synthesis,
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5.
the accumulation of effector molecules proceeds without protein synthesis in this strain after temperature shift up.
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6.
The effector substance seems not to be a protein.
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References
Chadwick, P., Pirrotta, V., Steinberg, R., Hopkins, N. Ptashne, M.: The λ and 434 phage repressors. Cold Spr. Harb. Symp. quant. Biol. 35, 283–294 (1970).
Cross, R., Lieb, M.: Heat sensitive early function in induced Nsus lysogens. J. Virol. 6, 33–41 (1970).
Dove, W.: Strains of phage Lambda in current use. Virology 38, 349–351 (1969).
Geissler, E.: Die lysogene Regulation und ihre Störung. Verh. Ges. exper. Med. 23, 21, 1969.
Goldthwait, D. A., Jacob, F.: Sur le méchanisme de l'induction du developpement du prophage chez les bactéries lysogénes. C. R. Acad. Sci. (Paris) 259, 661 (1964).
Horiuchi, T., Inokuchi, H.: Temperature sensitive regulation system of prophage Lambda induction. J. molec. Biol. 23, 217–224 (1967).
Klaus, S., Noack, D., Hüller, E.: Induction of λ CI prophages in Escherichia coli C 600 T44. Z. allgem. Mikrobiol. in press (1972).
Lanka, E., Schuster, H.: Replication of bacteriophages in Escherichia coli mutants thermosensitive in DNA synthesis. Molec. gen. Genet. 106, 274–285 (1970).
Lieb, M.: Allosteric properties of the λ repressor. J. molec. Biol. 39, 379–382 (1969).
Mikolajczyk, M., Schuster, H.: The fate of newly made DNA in Escherichia coli mutants thermosensitive in DNA synthesis. Molec. gen. Genet. 110, 272–288 (1971).
Monod, J., Wyman, J., Changeux, J. P.: Model for allosteric transition. J. molec. Biol. 12, 88–118 (1965).
Neuhard, J.: Studies on the acid-soluble nucleotide pool in thymine requiring mutants of E. coli during thymine starvation. III. On the regulation of the deoxyadenosine triphosphate and cytidine triphosphate pools of E. coli. Biochim. biophys. Acta (Amst.) 128, 104–115 (1966).
Neuhard, J., Münch-Peterson, A.: Studies on the acid-soluble nucleotide pool in thymine requiring mutants of E. coli during thymine starvation II. Changes in the amounts of deoxycytidine triphosphate and deoxyadenosine triphosphate in E. coli 15 T −A−U−. Biochim. biophys. Acta (Amst.) 114, 61–71 (1966).
Noack, D., Klaus, S.: In vivo control characteristic of the repression system in E. coli (λtU37) Z. allg. Mikrobiol. 9, 633–649 (1969).
Ptashne, M.: Isolation of the λ phage repressor. Proc. nat. Acad. Sci. (Wash.) 57, 306–312 (1966).
Ptashne, M.: Specific binding of the λ phage repressor to DNA. Nature (Lond.) 214, 232–235 (1967).
Ruff, W., Kirby, E. P., Goldthwait, D. A.: Cell division and prophage induction in E. coli: Studies of nucleotide levels. J. Bact. 106, 994–1004 (1971).
Tomizawa, J. I., Ogawa, T.: Effect of ultraviolet irradiation on bacteriophage Lambda immunity. J. molec. Biol. 23, 247–259 (1967).
Worcel, A.: Induction of chromosome reinitiation in a DNAts mutant of E. coli. J. molec. Biol. 52, 371–382 (1970).
Yoshikawa, H., Haas, M.: On the regulation of the initiation of DNA replication in bacteria. Cold Spr. Harb. Symp. quant. Biol. 33, 843–855 (1968).
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Communicated by G. Bertani
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Noack, D., Klaus, S. Inactivation kinetics of Lambda phage repressors in a mutant of E. coli temperature sensitive in DNA replication. Molec. Gen. Genet. 115, 216–224 (1972). https://doi.org/10.1007/BF00268885
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DOI: https://doi.org/10.1007/BF00268885