Skip to main content
SpringerLink
Log in

Conjugal transfer of UV-damaged F-prime sex factors and indirect induction of prophage λ

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Conjugal transfer of UV-damaged F-prime sex factors was investigated in connection with indirect induction. Using donors and recipients of different ability to perform pyrimidine dimer excision and/or recombination repair, it was found that:

  1. 1.

    Transfer of UV-irradiated F-prime DNA to the recipient cell is unaffected by the presence of pyrimidine dimers. The efficiency of transfer does not decrease even at rather high UV doses given to the donor in which, as a result of irradiation, chromosome replication is strongly inhibited.

  2. 2.

    Transfer of the signal responsible for indirect UV induction can be correlated with the transmission of the UV-damaged sex factor from donor to recipient.

  3. 3.

    In the recipient cell, the sex factor DNA does not undergo excision repair but can be repaired by recombination. It is inferred that the transferred DNA structure is not fully double-stranded.

  4. 4.

    In conclusion, indirect UV induction occurs in a λ-lysogenic recipient upon transmission of a UV damaged replicon which carries stabilized lesions as a consequence of its conjugal transfer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Appleyard, R. K., McGregor, J. F., Baird, K. M.: Mutation to extended host range and the occurrence of phenotypic mixing in the temperate coliphage lambda. Virology 2, 565 (1956).

    Google Scholar 

  • Arber, W.: Transduction of chromosomal genes and episomes in Escherichia coli. Virology 11, 273 (1960).

    Google Scholar 

  • Borek, E., Ryan, A.: The transfer of irradiation-elicited induction in a lysogenic organism. Proc. nat. Acad. Sci. (Wash.) 44, 374 (1958).

    Google Scholar 

  • —: The transfer of a biologically active irradiation product from cell to cell. Biochem. biophys. Acta (Amst.) 41, 67 (1960).

    Google Scholar 

  • Brooks, K., Clark, A. J.: Behavior of λ bacteriophage in a recombination deficient strain of E. coli. J. Virol. 1, 283 (1967).

    Google Scholar 

  • Campbell, A.: Sensitive mutants of bacteriophage λ. Virology 14, 22 (1961).

    Google Scholar 

  • Cuzin, F., Jacob, F.: Mutations de l'épisome F d'E. coli K-12, II: Mutants à réplication thermosensible. Ann. Inst. Pasteur 112, 397 (1967).

    Google Scholar 

  • Davis, J. E., Strauss, J. H., Jr., Sinsheimer, R. L.: Bacteriophage MS2: Another RNA phage. Science 134, 1427 (1961)

    Google Scholar 

  • Devoret, R., George, J.: Induction indirecte du prophage λ par le rayonnement ultraviolet. Mutation Res. 7, 713 (1967).

    Google Scholar 

  • — Monk, M., George, J.: Indirect ultraviolet induction of prophage λ and colicin I factor. Zbl. Bakt., I. Abt. Orig. 196, 193 (1965).

    Google Scholar 

  • -Devoret, R. Rörsch, A.: Cit. in: Mattern, I. E., Winden, M. P. van, Rörsch, A., The range of action of genes controlling radiation sensitivity in Escherichia coli. Mutation Res. 2, 111 (1965).

  • Dove, W. F.: Strains of phage lambda in current use. Virology 38, 349 (1969).

    Google Scholar 

  • George, J.: Corrélation entre la disparition de l'induction indirecte du prophage λ et la restriction du DNA transmis à la bactérie réceptrice inductible dans un croisement F+xF- hétérospécifique. C. R. Acad. Sci. (Paris) 262, 1805 (1965).

    Google Scholar 

  • Greenberg, J., Green, M. M. L., Bar-Nun, N.: The effect of UV irradiation on the capacity of an Hfr RecA strain of Escherichia coli to act as donor. Molec. Gen. Genetics 107, 209 (1970).

    Google Scholar 

  • Hertman, I., Luria, S. E.: Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage. J. molec. Biol. 23, 117 (1967).

    Google Scholar 

  • Howard-Flanders, P., Boyce, R. P.: DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat. Res. 6 (Suppl.) 156 (1966).

    Google Scholar 

  • — Rupp, W. D., Wilkins, B. M., Cole, R. S.: DNA replication and recombination after UV irradiation. Cold Spr. Harb. Symp. quant. Biol. 33, 195 (1968).

    Google Scholar 

  • — Theriot, L.: A method for selecting radiation sensitive mutants of Escherichia coli. Genetics 47, 1219 (1962).

    Google Scholar 

  • —, Stedeford, J. B.: Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12. J. Bact. 97, 1134 (1969).

    Google Scholar 

  • Jacob, F., Adelberg, E. A.: Transfert de caractères génétiques par incorporation au facteur sexuel d'Escherichia coli. C. R. Acad. Sci. (Paris) 249, 189 (1959).

    Google Scholar 

  • —, Campbell, A.: Sur le système de répression assurant l'immunité chez les bactéries lysogènes. C. R. Acad. Sci. (Paris) 248, 3219 (1959).

    Google Scholar 

  • —, Monod, J.: On the regulation of gene activity. Cold Spr. Harb. Symp. quant. Biol. 26, 193 (1961).

    Google Scholar 

  • —, Wollman, E. L.: Induction of phage development in lysogenic bacteria. Cold Spr. Harb. Symp. quant. Biol. 18, 101 (1953).

    Google Scholar 

  • — Sexuality and the genetics of bacteria, p. 196. New York: Academic Press 1961.

    Google Scholar 

  • Jansz, H. S., Pouwels, P. H., Rotterdam, V. van: Sensitivity to ultraviolet light of single—and double—stranded DNA. Biochim. biophys. Acta (Amst.) 76, 655 (1963).

    Google Scholar 

  • Kaiser, A. D.: Mutations in a temperate bacteriophage affecting its ability to lysogenize E. coli. Virology 3, 42 (1957).

    Google Scholar 

  • — Jacob, F.: Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology 4, 509 (1957).

    Google Scholar 

  • Lederberg, E. M., Lederberg, J.: Genetic studies of lysogenicity in E. coli. Genetics 38, 51 (1963).

    Google Scholar 

  • Luria, S. E.: Radiation and viruses in: Radiation biology, vol. II: Ultraviolet and related radiations (A. Hollaender). New York-Toronto-London: McGraw-Hill Book Company, Inc. (1955).

    Google Scholar 

  • Monk, M.: Induction of phage λ by transferred irradiated col I DNA. Molec. Gen. Genetics 106, 14 (1969).

    Google Scholar 

  • Pardee, A. B., Prestidge, L. S.: Ultraviolet-sensitive targets in the enzyme synthesizing apparatus of Escherichia coli. J. Bact. 93, 1210 (1967).

    Google Scholar 

  • Rosner, J. L., Kass, L. R., Yarmolinsky, M. B.: Parallel behaviour of F and P1 in causing indirect induction of lysogenic bacteria. Cold Spr. Harb. Symp. quant. Biol. 33, 785 (1968).

    Google Scholar 

  • Rupp, W. D., Howard-Flanders, P.: Discontinuities in the DNA synthesized in an excisiondefective strain of Escherichia coli following ultraviolet irradiation. J. molec. Biol. 31, 291 (1968).

    Google Scholar 

  • Setlow, J. K.: Photoreactivation. Radiat. Res. 6 (Suppl.) 141 (1966).

    Google Scholar 

  • Stacey, K. A., Evenchik, Z., Hayes, W.: The effects of ultraviolet irradiation on the fertility of F+ and Hfr strains of Escherichia coli K 12 defective for the repair of damaged DNA. J. gen. Microbiol. 59, 185 (1969).

    Google Scholar 

  • Weigle, J. J., Delbrück, M.: Mutual exclusion between an infecting phage and a carried phage. J. Bact. 62, 301 (1951)

    Google Scholar 

  • Wilkins, B. M., Howard-Flanders, P.: The genetic properties of DNA transferred from ultraviolet-irradiated Hfr cells of Escherichia coli K-12 during mating. Genetics 60, 243 (1968).

    Google Scholar 

  • Wood, W. B.: Host specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNA. J. molec. Biol. 16, 118 (1966).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Radiobiologie, Centre des Faibles Radioactivités, C.N.R.S., 91, Gif-sur-Y vette, France

    Jacqueline George & Raymond Devoret

Authors
  1. Jacqueline George
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Raymond Devoret
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Arber

Rights and permissions

Reprints and permissions

About this article

Cite this article

George, J., Devoret, R. Conjugal transfer of UV-damaged F-prime sex factors and indirect induction of prophage λ. Molec. Gen. Genet. 111, 103–119 (1971). https://doi.org/10.1007/BF00267786

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267786

Keywords

Search

Navigation