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Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence

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Summary

Revertants from streptomycin dependence to independence were isolated as single step mutants from six different streptomycin dependent strains. The ribosomal proteins from 100 such mutants were analyzed by two-dimensional polyacrylamide gel electrophoresis and some of them were also examined by immunological techniques. Altered proteins were found in 40 mutants, 24 in protein S4 and 16 in protein S5. No change in any other protein was detected.

Altered S5 proteins migrated into five different positions on the polyacrylamide plate and it can be concluded that the mutant proteins differ from the wild type probably by single amino acid replacements. The altered S4 proteins migrated into 17 different positions on the plate. Extensive changes of length, both shorter and longer than wild type S4 protein, are postulated for many of the mutant S4 proteins.

Analysis of the ribosomal proteins of four ram mutants revealed altered S4 protein in two of them. The alterations in these mutant proteins are probably very similar to those found in streptomycin independent mutants.

Among the revertants there was no apparent correlation between the protein alteration and the particular response to streptomycin.

These studies suggest a strong interaction between protein S12, which confers streptomycin dependence, and protein S4 or S5, which can suppress this dependence.

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References

  • Apirion, D.: Altered ribosomes in a suppressor strain of Escherichia coli. J. molec. Biol. 16, 285–301 (1966)

    Google Scholar 

  • Apirion, D., Schlessinger, D.: Reversion from streptomycin dependence in Escherichia coli by a further change in the ribosome. J. Bact. 94, 1275–1276 (1967)

    Google Scholar 

  • Apirion, D., Schlessinger, D., Phillips, S., Sypherd, P.: Escherichia coli: Reversion from streptomycin dependence, a mutation in a specific 30S ribosomal protein. J. molec. Biol. 43, 327–329 (1969)

    Google Scholar 

  • Birge, E. A., Kurland, C. G.: Altered ribosomal protein in streptomycin dependent Escherichia coli. Science 166, 1282–1284 (1969)

    Google Scholar 

  • Birge, E., Kurland, C. G.: Reversion of a streptomycin dependent strain of Escherichia coli. Molec. gen. Genet. 109, 356–369 (1970)

    Google Scholar 

  • Bjare, U., Gorini, L.: Drug dependence reversed by a ribosomal ambiguity mutation ram. J. molec. Biol. 57, 423–435 (1971)

    Google Scholar 

  • Brownstein, B. L., Lewandowski, L. J.: A mutation suppressing streptomycin dependence. I. An effect of ribosome function. J. molec. Biol. 25, 99–109 (1967)

    Google Scholar 

  • Daya-Grosjean, L., Garrett, R. A., Pongs, O., Stöffler, G., Wittmann, H. G.: Properties of the interaction of ribosomal protein S4 and 16S RNA in Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 119, 277–286 (1972)

    Google Scholar 

  • Deusser, E., Stöffler, G., Wittmann, H. G., Apirion, D.: Ribosomal proteins XVI. Altered S4 proteins in Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 109, 298–302 (1970)

    Google Scholar 

  • Donner, D., Kurland, C. G.: Changes in the primary structure of a mutational altered ribosomal protein S4 of Escherichia coli. Molec. gen. Genet. 115, 49–53 (1972)

    Google Scholar 

  • Funatsu, G., Puls, W., Schiltz, E., Reinbolt, J., Wittmann, H. G.: Ribosomal proteins, XXXI. Comparative studies on S4 proteins of six Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 115, 131–139 (1972)

    Google Scholar 

  • Funatsu, G., Schiltz, E., Wittmann, H. G.: Ribosomal proteins, XXVII. Localisation of the amino acid exchanges in protein S5 from Escherichia coli mutants resistant to spectinomycin. Molec. gen. Genet. 114, 106–111 (1971)

    Google Scholar 

  • Funatsu, G., Wittmann, H. G.: Ribosomal proteins XXXIII. Location of amino acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycin. J. molec. Biol. 68, 547–550 (1972)

    Google Scholar 

  • Garrett, R. A., Rak, K. H., Daya, L., Stöffler, G.: Ribosomal proteins, XXIX. Specific protein binding sites on 16S rRNA of Escherichia coli. Molec. gen. Genet. 114, 112–124 (1971)

    Google Scholar 

  • Green, M., Kurland, C. G.: A mutationally altered ribosomal protein with a defective RNA binding site. Nature (Lond.) New Biol. 234, 273–274 (1971)

    Google Scholar 

  • Hashimoto, K.: Streptomycin resistance in Escherichia coli analyzed by transductions. Genetics 45, 49–62 (1960)

    Google Scholar 

  • Hindennach, I., Stöffler, G., Wittmann, H. G.: Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli. Europ. J. Biochem. 23, 7–11 (1971)

    Google Scholar 

  • Itoh, T., Wittmann, H. G.: Amino acid replacements in protein S5 and S12 of two Escherichia coli revertants from streptomycin dependence to independence. Molec. gen. Genet. 127, 19–32 (1973)

    Google Scholar 

  • Kaltschmidt, E., Stöffler, G., Dzionara, M., Wittmann, H. G.: Ribosomal proteins XVII. Comparative studies on ribosomal proteins of four strains of Escherichia coli. Molec. gen. Genet. 109, 303–308 (1970)

    Google Scholar 

  • Kaltschmidt, E., Wittmann, H. G.: Ribosomal proteins, VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analyt. Biochem. 36, 401–412 (1970)

    Google Scholar 

  • Kreider, G., Brownstein, B. L.: A mutation suppressing streptomycin dependence, II. An altered protein in the 30S ribosomal subunit. J. molec. Biol. 61, 135–142 (1971)

    Google Scholar 

  • Kreider, G., Brownstein, B. L.: Ribosomal proteins involved in the suppression of streptomycin dependence in Escherichia coli. J. Bact. 109, 780–785 (1972)

    Google Scholar 

  • Mizushima, S., Nomura, M.: Assembly mapping of 30S ribosomal proteins from Escherichia coli. Nature (Lond.) 226, 1214–1218 (1970)

    Google Scholar 

  • Rosset, R., Gorini, L.: Ribosomal ambiguity mutation. J. molec. Biol. 39, 95–112 (1969)

    Google Scholar 

  • Schaup, H. W., Green, M., Kurland, C. G.: Molecular interactions of ribosomal components I. Identification of RNA binding sites for individual 30S ribosomal proteins. Molec. gen. Genet. 109, 193–205 (1970)

    Google Scholar 

  • Schaup, H. W., Green, M., Kurland, C. G.: Molecular interaction of ribosomal components. II. Site-specific complex formation between 30S proteins and ribosomal RNA. Molec. gen. Genet. 112, 1–8 (1971)

    Google Scholar 

  • Silengo, L., Schlessinger, D., Mangiarotti, G., Apirion, D.: Induction of mutations to streptomycin and spectinomycin in Escherichia coli by N-methyl-N′-nitro-N-nitrosoguanidine and acridine half mustard ICR 191. Mutation Res. 4, 701–703 (1967)

    Google Scholar 

  • Stöffler, G., Deusser, E., Wittmann, H. G., Apirion, D.: Ribosomal proteins XIX. Altered S5 ribosomal proteins in an Escherichia coli revertant from streptomycin dependence to independence. Molec. gen. Genet. 111, 334–341 (1971)

    Google Scholar 

  • Stöffler, G., Wittmann, H. G.: Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods. Proc. nat. Acad. Sci. (Wash.) 68, 2283–2287 (1971)

    Google Scholar 

  • Weisblum, B., Davies, J.: Antibiotic inhibitors of the bacterial ribosome. Bact. Rev. 32, 493–528 (1968)

    Google Scholar 

  • Wittmann, H. G., Stöffler, G., Hindennach, I., Kurland, C. G., Randall-Hazelbauer, L., Birge, E. A., Nomura, M., Kaltschmidt, E., Mizushima, S., Traut, R. P., Bickle, T. A.: Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories. Molec. gen. Genet. 111, 327–333 (1971)

    Google Scholar 

  • Wittmann-Liebold, B., Wittmann, H. G.: Ribosomal proteins, XX. Isolation and analysis of the tryptic peptides of proteins S5 from strains K and B of Escherichia coli. Biochim. biophys. Acta (Amst.) 251, 44–53 (1971)

    Google Scholar 

  • Zimmermann, R. A., Carvin, R. D., Gorini, L.: Alterations of a 30S ribosomal protein accompanying ram mutation in Escherichia coli. Proc. Nat. Acad. Sci. U.S. 68, 2263–2267 (1971)

    Google Scholar 

  • Zimmermann, R. A., Muto, A., Fellner, P., Ehresmann, C., Branlant, C.: Localization of ribosomal protein binding sites on 16S ribosomal RNA. Proc. Nat. Acad. Sci. U.S.A. 69, 1282–1286 (1972)

    Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für Molekulare Genetik, Berlin-Dahlem, Germany

    R. Hasenbank, C. Guthrie, G. Stöffler, H. G. Wittmann, L. Rosen & D. Apirion

  2. Department of Microbiology, Washington University Medical School, St. Louis, USA

    R. Hasenbank, C. Guthrie, G. Stöffler, H. G. Wittmann, L. Rosen & D. Apirion

  3. Department of Biochemistry and Biophysics, University of California San Francisco Medical Center, 94122, San Francisco, California, USA

    C. Guthrie

Authors
  1. R. Hasenbank
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  2. C. Guthrie
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  3. G. Stöffler
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  4. H. G. Wittmann
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  5. L. Rosen
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  6. D. Apirion
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Additional information

Communicated by E. Bautz

Paper No. 60 on “Ribosomal Proteins”. Preceding paper is by B. Wittmann-Liebold, Hoppe-Seyler's Z. physiol. Chemie, in press.

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Hasenbank, R., Guthrie, C., Stöffler, G. et al. Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Molec. Gen. Genet. 127, 1–18 (1973). https://doi.org/10.1007/BF00267778

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  • DOI: https://doi.org/10.1007/BF00267778

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