Skip to main content
SpringerLink
Log in

Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Mutations affecting sucrose metabolism have been mapped by PBS1 transduction on the Bacillus subtilis chromosome in seven loci sacA, sacB, sacQ, sacR, sacS, sacT and sacU. sacA and sacB are presumed to be the structural genes of a sucrase and a levansucrase respectively. sacR, sacS and sacT correspond to groups of mutations leading to constitutive synthesis of sucrase or both sucrase and levansucrase. In sacQ, sacS and sacU are located either mutations increasing the level of synthesis of levansucrase specifically (sacQ h, sacS h, sacU h) or mutations abolishing specifically the synthesis of levansucrase (sacU −). sacA, sacS and sacT map to the left of purA16. sacQ is located to the left of thr5, sacB and sacR between cysB3 and hisA1 and sacU between uvr1 and gtaB.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anagnostopoulos, C., Spizizen, J.: Requirements for transformation in Bacillus subtilis. J. Bact. 81, 741–746 (1961).

    Google Scholar 

  • Barat, M., Anagnostopoulos, C., Schneider, A. M.: Linkage relationships of genes controlling isoleucine, valine and leucine biosynthesis in Bacillus subtilis. J. Bact. 90, 357–369 (1965).

    Google Scholar 

  • Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315–323 (1956).

    Google Scholar 

  • Dedonder, R.: La lévane-sucrase de Bacillus subtilis. Bull. Soc. Chim. biol. (Paris) 42, 1745–1761 (1960).

    Google Scholar 

  • Dedonder, R., Lepesant, J.-A., Lepesant-Kejzlarová, J., Kunst, F., Pascal, M.: The control of the biosynthesis of sucrases in Bacillus subtilis. Genetic and biochemical aspects. In: Biochemistry of the glycoside linkage. Proceedings of the 2nd PAABS symposium. Academic Press (in press).

  • Demerec, M., Adelberg, E. A., Clark, A. J., Hartman, P. E.: A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76 (1966).

    Google Scholar 

  • Dubnau, D.: Linkage map of Bacillus subtilis. Handbook of biochemistry, selected data for molecular biology, ed. by H. A. Sober, second edit., I 39-I 45. Cleveland Ohio: Chemical Rubber Co. 1970.

    Google Scholar 

  • Dubnau, D., Goldthwaite, C., Smith, I., Marmur, J.: Genetic mapping in Bacillus subtilis. J. molec. Biol. 27, 163–185 (1967).

    Google Scholar 

  • Gay, P., Rapoport, G.: Etude des mutants dépourvus de fructose-1-phosphate kinase chez B. subtilis, C. R. Acad. Sci. (Paris) 271, 374–377 (1970).

    Google Scholar 

  • Georgopoulos, C. P.: Suppressor system in Bacillus subtilis 168. J. Bact. 97, 1397–1402 (1969).

    Google Scholar 

  • Grant, G. F., Simon, M. I.: Synthesis of bacterial flagella. II-PBS1 transduction of flagella specific markers in Bacillus subtilis. J. Bact. 99, 116–124 (1969).

    Google Scholar 

  • Harford, N., Sueoka, N.: Chromosomal location of antibiotic resistance markers in Bacillus subtilis. J. molec. Biol. 51, 267–286 (1970).

    Google Scholar 

  • Hoch, J. A., Anagnostopoulos, C.: Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis. J. Bact. 103, 295–301 (1970).

    Google Scholar 

  • Hoch, J. A., Barat, M., Anagnostopoulos, C.: Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J. Bact. 93, 1925–1937 (1967).

    Google Scholar 

  • Hoch, J. A., Mathews, J. L.: Genetic studies in Bacillus subtilis. Proceedings of the Vth international spore conference. Fontana, Wisconsin, U.S.A (U.S.A.), (1971).

  • Ivánovics, G., Csiszár, K.: Isolation and some characteristics of subtilis phages with transducing activity. Acta microbiol. Acad. Sci. hung. 9, 209–218 (1962).

    Google Scholar 

  • Kennett, R. H., Sueoka, N.: Gene expression during outgrowth of Bacillus subtilis spores. The relationship between gene order on the chromosome and temporal sequence of enzyme synthesis. J. molec. Biol. 60, 31–44 (1971).

    Google Scholar 

  • Lacks, S., Hotchkiss, R. D.: A study of the genetic material determining an enzyme activity in Pneumococcus. Biochim. biophys. Acta (Amst.) 39, 508–517 (1960).

    Google Scholar 

  • Lepesant, J.-A., Dedonder, R.: Transport du saccharose chez Bacillus subtilis. C. R. Acad. Sci. (Paris) 267, 1109–1112 (1968a).

    Google Scholar 

  • Lepesant, J.-A., Dedonder, R.: Isolement et caractérisation de mutants du système métabolique du saccharose chez Bacillus subtilis. C. R. Acad. Sci. (Paris) 266, 1442–1444 (1968b).

    Google Scholar 

  • Lepesant, J.-A., Kunst, F., Carayon, A., Dedonder, R.: Localisation génétique de mutants du système métabolique du saccharose chez Bacillus subtilis. Localisation par transduction à l'aide du phage PBS1. C. R. Acad. Sci. (Paris) 269, 1712–1715 (1969a).

    Google Scholar 

  • Lepesant, J.-A., Kunst, F., Carayon, A., Dedonder, R.: Localisátion génétique de mutants du système métabolique du saccharose chez Bacillus subtilis. Localisation par transformation. C. R. Acad. Sci. (Paris) 269, 1792–1794 (1969b).

    Google Scholar 

  • Murmur, J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. molec. Biol. 3, 208–218 (1961).

    Google Scholar 

  • Marquet, M., Wagner, M.-C., Delobbe, A., Rapoport, G.: Mise en évidence de systèmes de phosphotransférases dans le transport du glucose, du fructose et du saccharose chez Bacillus subtilis. C. R. Acad. Sci. (Paris) 271, 449–452 (1970).

    Google Scholar 

  • Masters, M., Broda, P.: Evidence for the bidirectional replication of the Escherichia coli chromosome. Nature (Lond.) New Biol. 232, 137–140 (1971).

    Google Scholar 

  • Masters, M., Pardee, A. B.: Sequence of enzyme synthesis and gene replication during the cell cycle of Bacillus subtilis. Proc. nat. Acad. Sci. (Wash.) 54, 64–70 (1965).

    Google Scholar 

  • Nester, E. W., Schafer, M., Lederberg, J.: Gene linkage in DNA transfer. A cluster of genes concerned with aromatic biosynthesis in Bacillus subtilis. Genetics 48, 529–551 (1963).

    Google Scholar 

  • Nishioka, Y., Eisenstark, A.: Sequence of genes replicated in Salmonella Thyphimurium as examined by transduction techniques. J. Bact. 102, 320–333 (1970).

    Google Scholar 

  • Okubo, S., Strauss, B., Stodolsky, H.: The possible role of recombination in the infection of competent Bacillus subtilis by bacteriophage deoxyribonucleic acid. Virology 24, 552–562 (1964).

    Google Scholar 

  • Okubo, S., Yanagida, T.: Isolation of a suppressor mutant in Bacillus subtilis. J. Bact. 95, 1187–1188 (1968).

    Google Scholar 

  • O'Sullivan, A., Sueoka, N.: Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment. J. molec. Biol. 27, 349–368 (1967).

    Google Scholar 

  • Pascal, M., Dedonder, R.: Purification et propriétés de la levane sucrase exocellulaire de Bacillus subtilis Marburg. Carbohydrate research (in Press).

  • Pascal, M., Kunst, F., Lepesant, J.-A., Dedonder, R.: Characterization of two sucrase activities in Bacillus subtilis. Marburg. Biochimie 53, 1059–1066 (1971).

    Google Scholar 

  • Prestidge, L. S., Spizizen, J.: Inducible sucrase activity in Bacillus subtilis distinct from levansucrase. J. gen. Microbiol. 59, 285–288 (1969).

    Google Scholar 

  • Raimondo, L. M., Lundh, N. P., Martinez, R. J.: Primary adsorption site of phage PBS1: the flagellum of Bacillus subtilis. J. Virol. 2, 256–264 (1968).

    Google Scholar 

  • Ramareddy, G., Reiter, H.: Sequential loss of loci in thymine-starved Bacillus subtilis 168 cells. Evidence for a circular chromosome. J. molec. Biol. 50, 525–532 (1970).

    Google Scholar 

  • Roseman, S.: The transport of carbohydrates by a bacterial phosphotransferase system. J. gen. Physiol. 54, 138 s-184 s (1969).

    Google Scholar 

  • Schaeffer, P., Ionesco, H., Ryter, A., Balassa, G.: La sporulation de Bacillus subtilis, étude génétique et physiologique. in Mécanismes de régulation des activités cellulaires chez les microorganismes, p. 553–563. Paris: Centre National de la Recherche Scientifique 1965.

    Google Scholar 

  • Schnös, M., Inman, R. B.: Position of branch points in replicating λ DNA. J. molec. Biol. 51, 61–73 (1970).

    Google Scholar 

  • Takahashi, I.: Transducing phages for Bacillus subtilis, J. gen. Microbiol. 31, 211–217 (1963).

    Google Scholar 

  • Yoshikawa, H.: temperature-sensitive mutants of Bacillus subtilis I-Multiforked replication and sequential transfer of DNA by a temperature-sensitive mutant. Proc. nat. Acad. Sci. (Wash.) 65, 206–213 (1970a).

    Google Scholar 

  • Yoshikawa, H.: Initiation of DNA replication in B. subtilis II-Linkage between termini of parental strands and origins of daughter strands. J. molec. Biol. 47, 403–417 (1970b).

    Google Scholar 

  • Young, F. E., Smith, C., Reilly, B. E.: Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. J. Bact. 98, 1087–1097 (1969).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department de Biochimie Cellulaire, Institut de Biologie Moléculaire, Centre National de la Recherche Scientifique, Paris

    Jean-Antoine Lepesant, Frank Kunst, Jana Lepesant-Kejzlarová & Raymond Dedonder

  2. Université Paris VII, Paris

    Jean-Antoine Lepesant, Frank Kunst, Jana Lepesant-Kejzlarová & Raymond Dedonder

Authors
  1. Jean-Antoine Lepesant
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Frank Kunst
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jana Lepesant-Kejzlarová
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Raymond Dedonder
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by P. Starlinger

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lepesant, JA., Kunst, F., Lepesant-Kejzlarová, J. et al. Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg. Molec. Gen. Genet. 118, 135–160 (1972). https://doi.org/10.1007/BF00267084

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267084

Keywords

Search

Navigation