Abstract
A CAPTURE ELISA/ELIFA system based on monoclonal capture and biotinylated monoclonal detection antibody is described. The assay is fast, highly specific and detects a minimum dose of 2500 Coxiella (C.) burnetii particles. In contrast to the sophisticated and cumbersome isolation procedures, even non-specialized laboratories could use this assay system for investigating clinical samples of different origin for C. burnetii within a short period of time.
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Thiele, D., Karo, M. & Krauss, H. Monoclonal antibody based capture ELISA/ELIFA for detection of Coxiella burnetii in clinical specimens. Eur J Epidemiol 8, 568–574 (1992). https://doi.org/10.1007/BF00146378
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DOI: https://doi.org/10.1007/BF00146378