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A novel blue light- and abscisic acid-inducible gene of Arabidopsis thaliana encoding an intrinsic membrane protein

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Abstract

Continuous irradiation with blue light (400–500 nm) induces flower formation in plantlets of Arabidopsis thaliana (C24) while red light (600–700 nm) is ineffective. This observation started a search for genes that are activated by blue light and initiate the morphogenic programme leading to flower formation. Several genes were identified via their cDNAs. From these clone AthH2, with an open reading frame for a hydrophobic 30.5 kDa polypeptide, was selected for further characterization of the corresponding gene. From a genomic library a DNA fragment of about 6.4 kb was isolated, comprising the coding region as well as 5′-upstream and 3′-downstream flanking segments. The coding region is composed of four exons, which specify a polypeptide of 286 amino acids. Several potential regulatory elements were found between position −670 and −1140 including GA and ABA sequence motifs. The latter could account for the observed induction of the AthH2 gene by ABA. Southern blot analysis of Arabidopsis genomic DNA suggests that the AthH2 gene is encoded by a single-copy gene. Hydropathy plots and secondary structure analysis of the putative polypeptide predict six membrane-spanning domains implicating a function as transmembrane channel protein. It displays significant homology with the proteins TR7a of pea (82%) and RD 28 of A. thaliana (68%).

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References

  1. Baker, ME, SaierJr, MH: A common ancestor for bovine lens fiber major intrinsic protein, soybean nodulin 26 protein, and E. coli glycerol faciliator. Cell 60: 185–186 (1990).

    Google Scholar 

  2. Bockholt, S, Kaldenhoff, R, Richter, G: Differential regulation of nuclear genes during blue light-dependent chloroplast differentiation in cultured plant cells. Bot Acta 104: 245–251 (1991).

    Google Scholar 

  3. Brown, JAM, Klein, WH: Photomorphogenesis in Arabidopsis thaliana (L.) Heynh. Threshold intensities and blue-far red synergism in floral induction. Plant Physiol 47: 393–399 (1971).

    Google Scholar 

  4. Cejudo, FJ, Ghose, TK, Stabel, P, Baulcombe, DC: Analysis of the gibberellin-responsive promoter of the cathepsin B-like gene from wheat. Plant Mol Biol 20: 849–856 (1992).

    Google Scholar 

  5. Davis, LG, Dibner, MD, Battey, JF (eds) Methods in Molecular Biology. Elsevier, New York (1986).

    Google Scholar 

  6. Duguid, JR, Rowhwer, RG, Seed, B: Isolation of cDNA of scrapie modulated RNAs by subtractive hybridization of a cDNA library. Proc Natl Acad Sci USA 85: 5738–5742 (1988).

    Google Scholar 

  7. Estelle, MA, Sommerville, C: Auxin-resistant mutants of Arabidopsis thaliana with altered morphology. Mol Gen Genet 206: 200–206 (1987).

    Google Scholar 

  8. Feinberg, AP, Vogelstein, B: A technique for radiolabelling restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).

    Google Scholar 

  9. Fickett, JW: Recognition of protein coding regions in DNA sequences. Nucl Acids Res 10: 5303–5318 (1982).

    Google Scholar 

  10. Fortin, MG, Morrison, NA, Verma, DPS: Nodulin 26, a peribacteroid membrane nodulin is expressed independently of the development of the peribacteroid compartment. Nucl acids Res 15: 813–824 (1987).

    Google Scholar 

  11. Frohmann, MA: RACE: Rapid amplification of cDNA ends. In: MAInnis, DHGelfand, JJSninsky, TJWhite (eds) PCR Protocols: A Guide to Methods and Application, pp. 28–38. Academic Press, San Diego (1990).

    Google Scholar 

  12. Gilmartin, PM, Sarokin, L, Memelink, I, Chua, NH: Molecular light switches for plant genes. Plant Cell 2: 369–378 (1990).

    Google Scholar 

  13. Gamble, PE, Mullet, J: Blue light regulates the accumulation of two psbD-psbC transcripts in barley chloroplasts. EMBO J 8: 2785–2793 (1989).

    Google Scholar 

  14. Gorin, MB, Yancey, CB, Cline, J, Revel, JP, Horwitz, J: The major intrinsic protein (MIP) of the bovine lens fiber membrane: characterization and structure based on cDNA cloning. Cell 39: 49–59 (1984).

    Google Scholar 

  15. Gubler, U, Hoffman, BJ: A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269 (1983).

    Google Scholar 

  16. Gubler, F, Jacobsen, JV: Gibberellin-responsive elements in the promoter of a barley high-pI-amylase gene. Plant Cell 4: 1435–1441 (1992).

    Google Scholar 

  17. Guerrero, FD, Jones, JT, Mullet, JE: Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes. Plant Mol Biol 15: 11–26 (1990).

    Google Scholar 

  18. Hoefte, H, Ludevic, D, Hubbard, L, Chrispeels, MJ: Vegetative and seed-specific isoforms of a putative solute transporter in the tonoplast of Arabidopsis thaliana. Plant Physiol 99: 561–570 (1992).

    Google Scholar 

  19. Hollander, MC, Fornacejr, AJ: Estimation of relative mRNA content by filter hybridization to a polythymidilate probe. Bio-Techniques 9: 174–179 (1990).

    Google Scholar 

  20. Huang, N, Sutliff, TD, Litss, JC, Rodriguez, RL: Classification and characterization of the rice-amylase multigene family. Plant Mol Biol 14: 655–668 (1990).

    Google Scholar 

  21. Jofuku, KD, Goldberg, RB: Analysis of plant gene structure. In: Shaw, CH (ed) Plant Molecular Biology: A Practical Approach, pp. 37–65. IRL Press, Oxford/Washington DC (1988).

    Google Scholar 

  22. Johnson, KD, Hoefte, H, Chrispeels, MJ: An intrinsic tonoplast protein of storage vacuoles in seeds is structurally related to a bacterial solute transporter (GlpF). Plant Cell 2: 525–532 (1990).

    Google Scholar 

  23. Kaldenhoff, R, Richter, G: Sequence of a cDNA for a novel-induced glycine-rich protein. Nucl Acids Res 17: 2853 (1989).

    Google Scholar 

  24. Kaldenhoff, R, Richter, G: Light induction of genes preceding chloroplast differentiation in cultured plant cells. Planta 181: 220–228 (1990).

    Google Scholar 

  25. Koorneef, M, Rolff, E, Spruit, CJP: Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana (L.) Heynh. Z Pflanzenphysiol 100: 147–160 (1980).

    Google Scholar 

  26. Krebs, O, Zimmer, K, Kwack, BH: A set-up for night-break experiments with different spectral composition of artificial light. Gartenbauwissenschaft 43: 120–125 (1987).

    Google Scholar 

  27. Liscum, E, Hangarter, RP: Arabidopsis mutants lacking blue light-dependent inhibition of hypocotyl elongation. Plant Cell 3: 685–694 (1991).

    Google Scholar 

  28. Luo, M, Liu, JH, Mohapatra, S, Hill, RD, Mohapatra, SS: Characterization of a gene family encoding abscisic acid- and environmental stress-inducible proteins of alfalfa. J Biol Chem 267: 15367–15374 (1992).

    Google Scholar 

  29. Marcotte, WR, Russel, SH, Quatrano, RS: Abscisic acid-responsive sequences from the EM gene of wheat. Plant Cell 1: 969–976 (1989).

    Google Scholar 

  30. Murray, MG, Thomson, WF: Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8: 4321 (1980).

    Google Scholar 

  31. Rao, Y, Jan, LY, Jan, YN: Similarity of the product of the Drosophila neurogenic gene big train to transmembrane channel proteins. Nature 345: 163–167 (1990).

    Google Scholar 

  32. Richards E: Preparation of genomic DNA from plant tissues. In: Current Protocols in Molecular Biology 1987–1988. Ausubel FM, Brent R, Kingston RE, Moore DD, Smith JA, Seidman JG, Struhl K (eds) pp. 231–233. Greene/Wiley Interscience (1987).

  33. Sambrook, J, Fritsch, EF, Maniatis, T: Molecular Cloning: A Laboratory Manual. Cold Spring Habor Laboratory Press, Cold Spring Habor, NY (1989).

    Google Scholar 

  34. Schweinfest, CW, Henderson, KW, Gu, JR, Kottaridis, SD, Besbeas, S, Panotopoulou, E, Papas, TS: Subtraction hybridization cDNA libraries from colon carcinoma and hepatic cancer. Genet Anal Techn Appl 7: 64–70 (1990).

    Google Scholar 

  35. Shepherd, JCW: Method to determine the reading frame of a protein from the purine/pyrimidine genome sequence and its possible evolutionary justification. Proc Natl Acad Sci USA 78: 1596–1600 (1981).

    Google Scholar 

  36. Sturzl, M, Roth, WK: ‘Run-off’ synthesis and application of defined single-stranded DNA hybridization probes. Anal Biochem 185: 164 (1990).

    Google Scholar 

  37. Tabor, S, Richardson, CC: DNA sequence analysis with a modified T7 DNA polymerase. Proc Natl Acad Sci USA 84: 4767–4771 (1987).

    Google Scholar 

  38. Yamaguchi-Shinozaki, K, Koizumi, M, Urao, S, Shinozaki, K: Molecular cloning and characterization of a 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana: Sequence analysis of one cDNA clone that encodes a putative transmembrane channel protein. Plant Cell Physiol 33: 217–224 (1992).

    Google Scholar 

  39. Yamamoto, YT, Cheng, CL, Conkling, MA: Root-specific genes from tobacco and Arabidopsis homologous to an evolutionary conserved gene family of membrane channel proteins. Nucl Acids Res 18: 7449 (1990).

    Google Scholar 

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  1. Institut für Botanik, Universität Hannover, D-30419, Hannover, Germany

    Ralf Kaldenhoff, Andreas Kölling & Gerhard Richter

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  1. Ralf Kaldenhoff
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  2. Andreas Kölling
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  3. Gerhard Richter
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Kaldenhoff, R., Kölling, A. & Richter, G. A novel blue light- and abscisic acid-inducible gene of Arabidopsis thaliana encoding an intrinsic membrane protein. Plant Mol Biol 23, 1187–1198 (1993). https://doi.org/10.1007/BF00042352

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  • DOI: https://doi.org/10.1007/BF00042352

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