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Effects of ambient ion concentrations on gill ATPases in fresh water eel,Anguilla anguilla

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Abstract

Branchial activities of Na+,K+-ATPase (ouabain sensitive), Mg2+ ATPase (ouabain insensitive) and kinetic analysis of high and low affinity Ca2+ ATPase were measured inAnguilla anguilla that had been acclimated to demineralized water (DW, Ca < 10 μM), freshwater (FW, Ca = 2 mM), and Low calcium freshwater (L-Ca, Ca = 0.9 mM). Na+,K+-ATPase activity decreased while ouabain insensitive activity increased when ambient Ca2+ decreased. Two kinetic forms of Ca2+ ATPase could be resolved in each environmental condition. The stimulation coefficients of both sites or enzymes were not affected by ambient Ca2+ concentrations. The maximal velocity of both the high and the low affinity Ca2+ ATPase was increased when external Ca2+ was decreased during acclimation. The low affinity Ca2+ ATPase and the Mg2+ stimulated enzyme could be a non specific enzyme accepting either Ca2+ or Mg2+. Results are compared with previous results in the literature and in relation to the branchial morphology and ionic exchanges in fish.

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  1. N. Mayer-Gostan

    Present address: Laboratoire de Physiologie Cellulaire et Comparée. Faculté des Sciences et des Techniques, Université de Nice, Parc Valrose, 06034, Nice Cedex

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  1. Laboratoire de Physiologie Cellulaire et Comparée et Laboratoire de Chimie moléculaire, Université de Nice Sophia Antipolis, Parc Valrose, 06034, Nice Cedex, France

    N. Mayer-Gostan & R. Naon

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  1. N. Mayer-Gostan
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Mayer-Gostan, N., Naon, R. Effects of ambient ion concentrations on gill ATPases in fresh water eel,Anguilla anguilla . Fish Physiol Biochem 10, 75–89 (1992). https://doi.org/10.1007/BF00004656

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