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Essential fatty acid requirement of juvenile red drum (Sciaenops ocellatus)

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Feeding experiments and laboratory analyses were conducted to establish the essential fatty acid (EFA) requirement of red drum (Sciaenops ocellatus). Juvenile red drum were maintained in aquaria containing brackish water (5 ± 2‰ total dissolved solids) for two 6-week experiments. Semipurified diets contained a total of 70% lipid consisting of different combinations of tristearin [predominantly 18:0] and the following fatty acid ethyl esters: oleate, linoleate, linolenate, and a mixture of highly unsaturated fatty acids (HUFA) containing approximately 60% eicosapentaenoate plus docosahexaenoate. EFA-deficient diets (containing only tristearin or oleate) rapidly reduced fish growth and feed efficiency, and increased mortality. Fin erosion and a “shock syndrome” also occurred in association with EFA deficiency. Of the diets containing fatty acid ethyl esters, those with 0.5–1% (n-3) HUFA (0.3–0.6% eicosapentaenoate plus docosahexaenoate) promoted the best growth, survival, and feed efficiency; however, the control diet containing 7% menhaden fish oil provided the best performance. Excess (n-3) HUFA suppressed fish weight gain; suppression became evident at 1.5% (n-3) HUFA, and was pronounced at 2.5%. Fatty acid compositions of whole-body, muscle and liver tissues from red drum fed the various diets generally reflected dietary fatty acids, but modifications of these patterns also were evident. Levels of saturated fatty acids appeared to be regulated independent of diet. In fish fed EFA-deficient diets (containing only tristearin or oleate), monoenes increased and (n-3) HUFA were preferentially conserved in polar lipid fractions. Eicosatrienoic acid [20:3(n-9)] was not elevated in EFA-deficient red drum, apparently due to their limited ability to transform fatty acids. Red drum exhibited some limited ability to elongate and desaturate linoleic acid [18:2(n-6)] and linolenic acid [18:3(n-3)]; however, metabolism of 18:3(n-3) did not generally result in increased tissue levels of (n-3) HUFA. Based on these responses, the red drum required approximately 0.5% (n-3) HUFA in the diet (approximately 7% of dietary lipid) for proper growth and health.

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References cited

  • Ackman, R.G. 1982. Fatty acid composition of fish oil. In Nutritional Evaluation of Long-Chain Fatty Acids in Fish Oil. pp. 25–88. Edited by S.M. Barlow and M.E. Stansby. Academic Press, London.

    Google Scholar 

  • Bautista, M.N. and De la Cruz, M.C. 1988. Linoleic (n-6) and linolenic (n-3) acids in the diet of fingerling milkfish (Chanos chanos Forsskal). Aquaculture 71: 347–358.

    Article  CAS  Google Scholar 

  • Bell, M.V., Henderson, R.J. and Sargent, J.R. 1986. The role of polyunsaturated fatty acids in fish. Comp. Biochem. Physiol. 83B: 711–719.

    Article  CAS  Google Scholar 

  • Bell, M.V., Henderson, R.J., Pirie, B.J.S. and Sargent, J.R. 1985. Effects of dietary polyunsaturated fatty acid deficiencies on mortality, growth and gill structure in the turbot, Scophthalmus maximus. J. Fish Biol. 26: 181–191.

    Article  CAS  Google Scholar 

  • Borlongan, I.G. 1992. The essential fatty acid requirement of milkfish (Chanos chanos Forsskal). Fish Physiol. Biochem. 9: 401–407.

    Article  CAS  Google Scholar 

  • Brenner, R.R. 1981. Nutritional and hormonal factors influencing desaturation of essential fatty acids. Prog. Lipid Res. 20: 41–47.

    Article  PubMed  CAS  Google Scholar 

  • Brenner, R.R. 1984. Effect of unsaturated fatty acids on membrane structure and enzyme kinetics. Prog. Lipid Res. 23: 69–96.

    Article  PubMed  CAS  Google Scholar 

  • Brenner, R.R., Garda, H., De Gomez Dumm, I.N.T. and Pezzano, H. 1981. Early effects of EFA deficiency on the structure and enzymatic activity of rat liver microsomes. In Essential Fatty Acids and Prostaglandins, Progress in Lipid Research. pp. 315–321. Edited by R.T. Holman. Pergamon Press, Oxford.

    Google Scholar 

  • Burr, G.O. and Burr, M.M. 1929. A new deficiency disease produced by the rigid exclusion of fat from the diet. J. Biol. Chem. 82: 345–367.

    CAS  Google Scholar 

  • Castell, J.D., Sinnhuber, R.O., Wales, J.H. and Lee, D.J. 1972a. Essential fatty acids in the diet of rainbow trout (Salmo gairdneri): Growth, feed conversion and some gross deficiency symptoms. J. Nutr. 102: 77–86.

    PubMed  CAS  Google Scholar 

  • Castell, J.D., Sinnhuber, R.O., Wales, J.H., and Lee, D.J. 1972b. Essential fatty acids in the diet of rainbow trout (Salmo gairdneri): Physiological symptoms of EFA deficiency. J. Nutr. 102: 87–92.

    PubMed  CAS  Google Scholar 

  • Castell, J.D., Lee, D.J. and Sinnhuber, R.O. 1972c. Essential fatty acids in the diet of rainbow trout (Salmo gairdneri): Lipid metabolism and fatty acid composition. J. Nutr. 102: 93–100.

    PubMed  CAS  Google Scholar 

  • Cowey, C.B. and Sargent, J.R. 1972. Fish nutrition. Mar. Biol. 10: 383–492.

    Article  CAS  Google Scholar 

  • Cowey, C.B., Owen, J.M., Adron, J.W. and Middleton, C. 1976. Studies on the nutrition of marine flatfish. The effect of different dietary fatty acids on the growth and fatty acid composition of turbot Scophthalamus maximus. Br. J. Nutr. 36: 479–486.

    Article  PubMed  CAS  Google Scholar 

  • Cowey, C.B. and Sargent, J.R. 1977. Lipid nutrition in fish. Comp. Biochem. Physiol. 57B: 269–273.

    Article  CAS  Google Scholar 

  • Farkas, T., Csengeri, I., Majoros, F. and Olah, J. 1977. Metabolism of fatty acids in fish. I. Development of essential fatty acid deficiency in the carp, Cyprinus carpio Linnaeus 1758. Aquaculture 11: 147–157.

    Article  CAS  Google Scholar 

  • Folch, J., Lees, M. and Sloane-Stanley, G.H. 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226: 497–509.

    PubMed  CAS  Google Scholar 

  • Fujii, M., Nakayama, H. and Yone, Y. 1976. Effect of n-3 fatty acids on growth, feed efficiency and fatty acid composition of red sea bream (Chyrysophrys major). Rep. Fish. Res. Lab. Kyushu Univ. 3: 65–86.

    CAS  Google Scholar 

  • Gatlin, D.M., III, MacKenzie, D.S., Craig, S.R. and Neill, W.H. 1992. Effects of dietary sodium chloride on red drum juveniles in waters of various salinities. Prog. Fish-Cult., 54: 220–227.

    Article  Google Scholar 

  • Greene, D.H.S. and Selivonchick, D.P. 1987. Lipid metabolism in fish. Prog. Lipid Res. 26: 53–85.

    Article  PubMed  CAS  Google Scholar 

  • Hardy, R.W., Scott, T.M. and Harrell, L.W. 1987. Replacement of herring oil with menhaden oil, soybean oil, or tallow in the diets of Atlantic salmon raised in marine netpens. Aquaculture 65: 267–277.

    Article  Google Scholar 

  • Kanazawa, A. 1985. Essential fatty acid and lipid requirement of fish. In Nutrition and Feeding in Fish. pp. 281–298. Edited by C.B. Cowey, A.M. Mackie and J.G. Bell. Academic Press, London.

    Google Scholar 

  • Kanazawa, A., Teshima, S., Sakamoto, M. and Awal, M.A. 1980. Requirement of Tilapia zillii for essential fatty acids. Bull. Jap. Soc. Sci. Fish. 46: 1353–1356.

    CAS  Google Scholar 

  • Lee, A.G., East, J.M. and Fround, R.J. 1986. Are essential fatty acids essential for membrane function? Prog. Lipid Res. 25: 41–46.

    Article  PubMed  CAS  Google Scholar 

  • Lochmann, R.T. and Gatlin, D.M., III. 1993. Evaluation of different types and levels of triglycerides, singly and in combination with different levels of n-3 highly unsaturated fatty acid ethyl esters in diets of juvenile red drum, Sciaenops ocellatus. Aquaculture, (In press).

  • McClain, W.R. and Gatlin, D.M., III. 1988. Dietary zinc requirement of Oreochromis aureus and effects of dietary calcium and phytate on zinc bioavailability. J. World Aquacult. Soc. 19: 103–108.

    Google Scholar 

  • Mead, J.F., Alfin-Slater, R.B., Howton, D.R. and Popjak, G. 1986. Lipids, Chemistry, Biochemistry and Nutrition. Plenum Press, New York.

    Google Scholar 

  • Moon, H.Y. and Gatlin, D.M., III. 1991. Total sulfur amino acid requirement of the red drum (Sciaenops ocellatus). Aquaculture 95: 97–106.

    Article  CAS  Google Scholar 

  • Mugrditchian, D.S., Hardy, R.W. and Iwaoka, W.T. 1981. Lin-seed oil and animal fat as alternative lipid sources in dry diets for chinook salmon (Oncorhynchus tshawytscha). Aquaculture 25: 161–172.

    Article  Google Scholar 

  • Osmundsen, H. 1982. Peroxisomal beta-oxidation of long chain fatty acids: effect of a high fat diet. Ann. N.Y. Acad. Sci. 86: 13–29.

    Google Scholar 

  • Owen, J.M., Adron, J.W., Sargent, J.R. and Cowey, C.B. 1972. Studies on the nutrition of marine flatfish. The effect of dietary fatty acids on the tissue fatty acids of the plaice, Pleuronectes platessa. Mar. Biol. 13: 160–166.

    Article  CAS  Google Scholar 

  • SAS Institute, Inc. 1985. SAS User's Guide: Statistics, version 5 edn. Cary, NC.

  • Satoh, S., Poe, W.E. and Wilson, R.P. 1989. Effect of dietary n-3 fatty acids on weight gain and liver polar lipid fatty acid composition of fingerling channel catfish. J. Nutr. 119: 23–28.

    PubMed  CAS  Google Scholar 

  • Serrano, J.A., Nematipour G.R. and D.M. Gatlin, III. 1992. Dietary protein requirement of the red drum (Sciaenops ocellatus) and relative use of dietary carbohydrate and lipid. Aquaculture 101: 283–291.

    Article  CAS  Google Scholar 

  • Sinnhuber, R.O. 1969. The role of fats. In Fish in Research. pp. 245–261. Edited by O.W. Neuhaus and J.E. Halver. Academic Press, New York.

    Google Scholar 

  • Stickney, R.R. and Andrews, J.W. 1971. Combined effects of dietary lipids and environmental temperature on growth, metabolism and body composition of channel catfish (Ictalurus punctatus). J. Nutr. 101: 1703–1710.

    CAS  Google Scholar 

  • Takeuchi, T. and Watanabe, T. 1976. Nutritive value of n-3 highly unsaturated fatty acids in pollack liver oil for rainbow trout. Bull. Jap. Soc. Sci. Fish. 42: 907–919.

    Google Scholar 

  • Takeuchi, T. and Takeuchi, T., Arai, S., Watanabe, T. and Shimma, Y. 1980. Requirement of eel Anguilla japonica for essential fatty acids. Bull. Jap. Soc. Sci. Fish. 46: 345–353.

    CAS  Google Scholar 

  • Takeuchi, T., Toyota, M., Satoh, S. and Watanabe, T. 1990. Requirement of juvenile red seabream Pagrus major for eicosapentaenoic and docosahexaenoic acids. Bull. Jap. Soc. Sci. Fish. 56: 1263–1269.

    CAS  Google Scholar 

  • Watanabe, T. 1979. Effect of excess amounts of essential fatty acids on growth of rainbow trout. Bull. Jap. Soc. Sci. Fish. 45: 1517–1519.

    Google Scholar 

  • Takeuchi, T., Watanabe, T. and Nose, T. 1979. Requirement for essential fatty acids of chum salmon (Oncorhynchus keta) in freshwater environment. Bull. Jap. Soc. Sci. Fish. 45: 1319–1323.

    CAS  Google Scholar 

  • Watanabe, T., Takashima, F. and Ogino, C. 1974. Effect of dietary methyl linolenate on growth of rainbow trout. Bull. Jap. Soc. Sci. Fish. 40: 181–188.

    CAS  Google Scholar 

  • Watanabe, T. 1982. Lipid nutrition in fish. Comp. Biochem. Physiol. 73B: 3–15.

    Article  Google Scholar 

  • Watanabe, T., Takeuchi, T., Arakawa, T., Imaizumi, K., Sekiya, S. and Kitajima, C. 1989. Requirement of juvenile striped jack Longirostris delicatissimus for n-3 highly unsaturated fatty acids. Bull. Jap. Soc. Sci. Fish. 55: 1111–1117.

    CAS  Google Scholar 

  • Williams, C.D. and Robinson, E.H. 1988. Response of red drum to various dietary levels of menhaden oil. Aquaculture 70: 107–120.

    Article  CAS  Google Scholar 

  • Yone, Y. and Fujii, M. 1975. Studies on nutrition of red sea bream-:XI. Effect of n-3 fatty acid supplement in a corn oil diet on growth rate and feed efficiency. Bull. Jap. Soc. Sci. Fish. 41: 73–77.

    CAS  Google Scholar 

  • Yone, Y. 1978. Essential fatty acids and lipid requirements of marine fish. In Dietary Lipids in Aquaculture. pp. 43–59. Edited by the Bulletin of the Japanese Society of Scientific Fisheries. Koseisha-Koseikaku, Tokyo.

    Google Scholar 

  • Yu, T.C. and Sinnhuber, R.O. 1979. Effect of dietary n-3 and n-6 fatty acids on growth and feed conversion efficiency of coho salmon (Oncorhynchus kisutch). Aquaculture 16: 31–38.

    Article  CAS  Google Scholar 

  • Yu, T.C., Sinnhuber, R.O. and Putnam, G.B. 1977. Effect of dietary lipids on fatty acid composition of body lipid in rainbow trout (Salmo gairdneri). Lipids 12: 495–499.

    PubMed  CAS  Google Scholar 

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  1. Department of Wildlife and Fisheries Sciences, Texas A&M University System, College Station, TX, 77843-2258, USA

    Rebecca T. Lochmann & Delbert M. Gatlin III

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  1. Rebecca T. Lochmann
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  2. Delbert M. Gatlin III
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Lochmann, R.T., Gatlin, D.M. Essential fatty acid requirement of juvenile red drum (Sciaenops ocellatus). Fish Physiol Biochem 12, 221–235 (1993). https://doi.org/10.1007/BF00004370

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