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Gill epithelial cells kinetics in a freshwater teleost, Oncorhynchus mykiss during adaptation to ion-poor water and hormonal treatments

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Abstract

The aim of this work was to determine the kinetics of the dramatic development of the gill chloride cells (CCs) during adaptation of the salmonid Oncorhynchus mykiss to an ion-poor environment.

To monitor cell division, the incorporation in the mitotic cell DNA of bromo-deoxyuridine (BrdUrd) was visualized with a monoclonal antibody. The density of labelled nuclei was used as an index of cellular division (proliferation), concomitantly with morphometry of phenotypic changes monitored with SEM.

In the filament epithelium, a phase of CC differentiation occurred within 12h after the transfer, followed by a delayed phase of cell proliferation (48h). In the lamellar epithelium, the present study demonstrates the absence of cell proliferation after ion-poor water transfer. The conclusion is that proliferation (mitosis) is important in the primary filament whereas differentiation and migration (from the filament) is the main mechanism for the appearance of CCs on the secondary lamellae.

The present study suggests that cortisol promoted differentiation, but not division, of cells. CCs, presumably premature, were stained by anti-cortisol monoclonal antibody indicating the presence of cortisol. No mature CCs were stained.

Growth hormone (oGH, ratGH) increased the rate of cell division both in lamellar and filament epithelium.

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  1. Laboratoire de Morphologie Fonctionnelle des Adaptations Centre d'Ecologie et de Physiologie Energétiques, C.N.R.S., 23, Rue du Loess, 67037, Strasbourg Cedex, France

    Pierre Laurent, Suzanne Dunel-Erb, Claudine Chevalier & Jacques Lignon

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  1. Pierre Laurent
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  2. Suzanne Dunel-Erb
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  3. Claudine Chevalier
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  4. Jacques Lignon
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Laurent, P., Dunel-Erb, S., Chevalier, C. et al. Gill epithelial cells kinetics in a freshwater teleost, Oncorhynchus mykiss during adaptation to ion-poor water and hormonal treatments. Fish Physiol Biochem 13, 353–370 (1994). https://doi.org/10.1007/BF00003415

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