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Selection Methods for High-Producing Mammalian Cell Lines

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Cell Line Development

Part of the book series: Cell Engineering ((CEEN,volume 6))

Abstract

The continually expanding market for biotherapeutics such as recombinant proteins that are produced in mammalian cell cultures and the relatively high clinical doses required of these therapeutics is predicted to lead to a bioreactor capacity crunch. Current estimates suggest that by the turn of the decade worldwide bioreactor capacity, currently standing at approximately 500,000 L, will no longer be able to meet demand. Many advances have been made in process design and medium formulation yet there is still scope to improve specific productivities of these manufacturing cell lines. An important step in this process is the selection of a high producing clone from cell lines that are often highly heterogeneous with regard to productivity this can be a difficult task however given the sheer volume of cells that need to be screened. Here we summarise some of the various methods currently available for the isolation of highly productive clonal cell lines.

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Acknowledgements

We thank James C. Weaver, Division of Health Sciences and Technology, MIT; Tim Ward, The Automation Partnership; and James Fandl, Regeneron Pharmaceuticals for provision of information. We also thank Science Foundation Ireland (SFI) for funding.

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  1. School of Chemical and Bioprocess Engineering, and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland

    S. M. Browne & M. Al-Rubeai

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  1. S. M. Browne
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Correspondence to M. Al-Rubeai .

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  1. Dept. Chemical & Biochemical, University College, Dublin, Belfield, Dublin 4, Ireland

    Mohamed Al-Rubeai

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Browne, S.M., Al-Rubeai, M. (2009). Selection Methods for High-Producing Mammalian Cell Lines. In: Al-Rubeai, M. (eds) Cell Line Development. Cell Engineering, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2245-5_7

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