Abstract
Comparative genomic hybridization (CGH) can be used for comprehensive low-resolution analysis of chromosomal imbalances of an entire human genome. Genomic DNAs from a tested specimen (test DNA) and a normal one (reference DNA) are differentially labeled and simultaneously hybridized to normal metaphase spreads. The ratio of fluorescence intensities along each normal chromosome is analyzed and allows the detection of regions that are over- or underrepresented in the tested specimen. Even though higher-resolution array CGH is nowadays widely used instead of CGH, this molecular cytogenetic approach should not be forgotten, as it can be performed also by labs only equipped with a 3-filter fluorescence microscope. In this chapter we describe the technique of CGH itself and a variant of it. The so-called microdissection-based comparative genomic hybridization (micro-CGH) technique allows the molecular cytogenetic investigation of harvested and cytogenetically fixed interphase nuclei. Micro-CGH has been already used in leukemia cytogenetics as well as clinical genetics.
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Liehr, T., Glaser, A., Kosyakova, N. (2017). Comparative Genomic Hybridization (CGH) and Microdissection-Based CGH (Micro-CGH). In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_54
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DOI: https://doi.org/10.1007/978-3-662-52959-1_54
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