Abstract
A major problem in the recently developed hybridoma technology (1) is screening very large numbers of culture supernatants for antibodies to cell surfaces. Whilst the radioactive anti-immunoglobulin (Ig) binding method has the advantage of detecting all Ig isotopes, it is nonetheless more time consuming than cytotoxic procedures. In addition it is inconvenient for detecting alloantibodies to B lymphocytes since of necessity the radioactive anti-Ig probe will bind to pre-existing B cell sIg. The modification to pre-existing procedures reported here were designed to meet these problems.
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References
Köhler, G., Milstein, C.: Continuous cultures of fused cells secreting antibody of defined specificity. Nature. 256, 495–497 (1975).
Laskey, R. A., Mills, A. D.: Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitised film. FEBS Lett. 82, 314–316 (1977).
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© 1979 Springer-Verlag Berlin Heidelberg
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Parkhouse, R.M.E., Guarnotta, G. (1979). Rapid Binding Test for Detection of Alloantibodies to Lymphocyte Surface Antigens. In: Melchers, F., Potter, M., Warner, N.L. (eds) Lymphocyte Hybridomas. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67448-8_21
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DOI: https://doi.org/10.1007/978-3-642-67448-8_21
Publisher Name: Springer, Berlin, Heidelberg
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