Abstract
Stromal vascular fraction (SVF) of adipose tissue consists of the connective support for the adipocytes, and it is proven to contain mesenchymal stem cells. SVF is commonly isolated by separation, collagenase digestion, and filtration processes in order to obtain a cell suspension to be cultivated, but this process takes 8–10 h and therefore is difficult to apply in clinical practice. On the basis of a new protocol and the ability to directly isolate mesenchymal stem cells from adipose tissue through their adherence to the cultivated area, a new low-invasive technique is now available through the use of a new system (myStem Evo) which allows separation, washing, and concentration of the cellular components on the basis of cell size directly inside a completely closed apparatus, without any centrifugation, with minimal handling and in absolute sterility, obtaining a final product rich in multipotent stem cells ready to use in operating room for one-step chondral defect repair. This single-step ADSC implantation procedure reduces time and costs and is less invasive to the patient. Recently, the literature has demonstrated an incredible interest in the use of ADSCs in regenerative medicine but, although extremely promising, needs more patients and longer follow-ups.
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Sciarretta, F.V., Ascani, C. (2014). Adipose Tissue and Progenitor Cells for Cartilage Formation. In: Doral, M., Karlsson, J. (eds) Sports Injuries. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-36801-1_159-1
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DOI: https://doi.org/10.1007/978-3-642-36801-1_159-1
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