Skip to main content

In Vitro Digestibility of Native Grass Silage Supplemented with Leucaena leucocephala

  • Conference paper
  • First Online:
Proceeding of the 2nd International Conference on Tropical Agriculture

Abstract

Research has been conducted to determine the best level of Leucaena leucocephala supplementation to digestibility and fermentability in vitro. The study was designed using completely randomized design with three levels supplementation of Leucaena (10%, 20%, and 30%) and each treatment was done in six replications. All of the silage treatments was inoculated with 2% Lactobacillus plantarum, and stored in mini-silos for 21 days. The results showed that supplementing native grass with 20% Leucaena resulted in the most significant (P < 0.05) dry matter and organic matter digestibility. The highest fiber crude digestibility was obtained at 30% supplementation. However, the highest digestibility of crude protein obtained at 10% Leucaena supplementation and the digestibility decreased linearly with the increasing levels of supplementation (P < 0.05). These data indicate that supplementing native grass with 20% Leucaena is the best treatment in terms of nutrient digestibility.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Abbreviations

CF:

crude fiber

CFD:

crude fiber digestibility

CP:

crude protein

CPD:

crude protein digestibility

DM:

dry matter

DMD:

dry matter digestibility

EE:

Ether Extract

IVCFD:

in vitro crude fiber digestibility

IVCPD:

in vitro crude protein digestibility

IVDMD:

in vitro dry matter digestibility

IVOMD:

in vitro organic matter digestibility

LAB:

lactic acid bacteria

LIPI:

Lembaga Ilmu Pengetahuan Indonesia

NFE:

Nitrogen free extract

OM:

organic matter

OMD:

organic matter digestibility

PEG:

polyethylene glycol

TDN:

total digestible nutrient

UGM:

Universitas Gadjah Mada

References

  1. Yasin S. Perilaku makan ruminansia sebagai bioindikator fenologi dan dinamika padang penggembalaan [Ingestive behaviour of ruminant as bioindicator of phenology and dynamics of grassland]. Pastura 2013;3(1):1–4. (in Bahasa Indonesia).

    Google Scholar 

  2. National Research Council. Nutrient requirements of beef cattle. 7th rev. ed. Washington, DC: The National Academies Press; 1996.

  3. Souza WF, Pereira OG, Ribeiro KG, Santos SA, Valadares-Filho SC. Intake, digestibility, nitrogen efficiency, and animal performance of growing and finishing beef cattle fed warm-season legume (Stylosanthes capitata plus Stylosanthes macrocephala) silage replacing corn silage. J Anim Sci. 2014;92(9):4099–107.

    Article  CAS  PubMed  Google Scholar 

  4. Gusha J, Halimani TE, Ngongoni NT, Ncube S. Effect of feeding cactus-legum silages on nitrogen retention, digestibility and microbial protein synthesis in goats. Anim Feed Sci Technol. 2015;206:1–7.

    Article  CAS  Google Scholar 

  5. Yunus M. Pengaruh pemberian daun lamtoro (Leucaena leucocephala) terhadap kualitas silase rumput gajah (Pennisetum purpereum) yang diberi molasses [The effect of gift lamtoro leaf (Leucaena leocephala) to quality of silage of elephant grass (Pennisetum purpereum) that adding molasses)]. Agripet. 2009;9:38–42. (in Bahasa Indonesia).

    Google Scholar 

  6. Association of Official Analytical Chemist. Official method of analysis. 18th ed. Washington, DC: The Association of Official Analytical Chemist; 2005.

    Google Scholar 

  7. Tilley JMA, Terry RA. A two-stage technique for the in vitro digestion of forage crop. J Br Grassl Soc. 1963;18:104–11.

    Article  CAS  Google Scholar 

  8. Makkar HPS. In vitro screening of feed resources for efficiency of microbial protein synhesis (Chapter 7). In: Vercoe PE, Makkar HPS, Schlink AC, editors. In vitro screening of plant resources for extra-nutritional attributes in ruminants: nuclear and related methodologies. Netherlands: Springer; 2010. p. 107–144.

    Google Scholar 

  9. Hartadi H, Reksohadiprodjo S, Tillman AD. Tabel komposisi pakan untuk Indonesia [Indonesian feed composition tables]. Yogyakarta: UGM Press; 2005. (in Bahasa Indonesia).

    Google Scholar 

  10. Tan HY, Sieo CC, Abdullah N, Liang JB, Huang XD, Ho YW. Effects of condensed tannins from Leucaena on methane production, rumen fermentation, and populations of methanogens and protozoa in vitro. Anim Feed Sci Technol. 2011;169:185–93.

    Article  CAS  Google Scholar 

  11. Niderkorn V, Mueller-Harvey I, Morvan AL, Aufrere J. Synergistic effects of mixing cocksfoot and sainfoin on in vitro rumen fermentation. Role of condensed tannins. Anim Feed Sci. 2012;178(1–2):48–56.

    Article  CAS  Google Scholar 

  12. Galindo J, Marrero Y, Ruiz TE, González N, Díaz A, Aldama AI, et al. Effect of a multiple mixture of herbaceous legumes and Leucaena leucocephala on the microbial population and fermentative products in the rumen of Zebu upgraded yearling steers. Cuba J Agric Sci. 2009;43:251–7.

    Google Scholar 

  13. Min BR, Attwood GT, Reilly K, Sun W, Peters JS, Barry TN, et al. Lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep. Can J Anim Sci. 2002;48:911–21.

    CAS  Google Scholar 

  14. Toral PG, Hervas G, Missaoui H, Andres S, Giraldez FJ, Jellali S, et al. Effects of a tannin-rich legume (Onobrychis viciifolia) on in vitro ruminal biohydrogenation and fermentation. Span J Agric Res. 2016;14(1):e0602. https://doi.org/10.5424/sjar/2016141-8989.

    Article  Google Scholar 

  15. McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA, Sinclair LA, Wilkinson RG. Animal nutrition. 7th ed. London: Prentice Hall; 2011.

    Google Scholar 

  16. Goel G, Puniya AK, Aguilar CN, Singh K. Review: interaction of gut microflora with tannins in feed. Naturwissenchaften. 2005;92:497–503.

    Article  CAS  Google Scholar 

  17. Barros-Rodríguez MA, Solorio-Sánchez FJ, Sandoval-Castro CA, Klieve A, Rojas-Herrera RA, Briceño-Poot EG, et al. Rumen function in vivo and in vitro in sheep fed Leucaena leucocephala. Trop Anim Health Prod. 2015;47:757–64.

    Article  PubMed  Google Scholar 

  18. Tillman AD, Hartadi H, Reksohadiprodjo S, Lebdosoekojo S. Ilmu makanan ternak dasar [Principle of animal nutrition]. Yogyakarta: UGM Press; 1991. (in Bahasa Indonesia).

    Google Scholar 

Download references

Acknowledgments

Thanks to Indonesia Endowment Fun for Education, the Ministry of Finance, The Republic of Indonesia as the main sponsor of this research, either in the financial support and the moral support. (Kep-105/LPDP/2014)

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Cuk Tri Noviandi .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Nature Switzerland AG

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Putra, R.A., Noviandi, C.T., Umami, N. (2018). In Vitro Digestibility of Native Grass Silage Supplemented with Leucaena leucocephala . In: Sukartiko, A., Nuringtyas, T., Marliana, S., Isnansetyo, A. (eds) Proceeding of the 2nd International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-97553-5_23

Download citation

Publish with us

Policies and ethics