Skip to main content
SpringerLink
Log in

Biological Small-Angle X-Ray Scattering (SAXS)

  • Chapter
  • First Online:
Plant Structural Biology: Hormonal Regulations

  • 1133 Accesses

Abstract

Small angle X-ray scattering (SAXS) method for studies of biological macromolecules in solution is briefly described. A short introduction to the basic physical principles is followed by the description of SAXS experiment and an overview of modeling approaches used in scattering data analysis. Practical aspects, advantages, and limitations of the method are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Berman HM (2000) The Protein Data Bank. Nucleic Acids Res 28:235–242

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bernadó P, Mylonas E, Petoukhov MV, Blackledge M, Svergun DI (2007) Structural characterization of flexible proteins using small-angle X-ray scattering. J Am Chem Soc 129:5656–5664

    Article  CAS  PubMed  Google Scholar 

  • Debye PZ v R (1915) Ann Phys 351:809–823

    Article  Google Scholar 

  • Glatter O, Kratky O (1982) Structure analysis by small-angle X-ray and neutron scattering. Academic Press, New York

    Google Scholar 

  • Grishaev A, Guo L, Irving T, Bax A (2010) Improved fitting of solution X-ray scattering data to macromolecular structures and structural ensembles by explicit water modeling. J Am Chem Soc 132:15484–15486

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guinier A (1939) La diffraction des rayons X aux très petits angles : application à l’étude de phénomènes ultramicroscopiques. Ann Phys 11:161–237

    Article  Google Scholar 

  • Jeffries CM et al (2016) Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11:2122–2153

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Konarev PV, Volkov VV, Sokolova AV, Koch MHJ, Svergun DI (2003) PRIMUS: a windows PC-based system for small-angle scattering data analysis. J Appl Crystallogr 36:1277–1282

    Article  CAS  Google Scholar 

  • Mylonas E, Svergun DI (2007) Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering. J Appl Crystallogr 40:s245–s249

    Article  CAS  Google Scholar 

  • Ozenne V et al (2012) Flexible-meccano: a tool for the generation of explicit ensemble descriptions of intrinsically disordered proteins and their associated experimental observables. Bioinformatics 28:1463–1470

    Article  CAS  PubMed  Google Scholar 

  • Panjkovich A, Svergun DI (2016) Deciphering conformational transitions of proteins by small angle X-ray scattering and normal mode analysis. Phys Chem Chem Phys 18:5707–5719

    Article  CAS  PubMed  Google Scholar 

  • Petoukhov MV, Svergun DI (2005) Global rigid body modeling of macromolecular complexes against small-angle scattering data. Biophys J 89:1237–1250

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Petoukhov MV et al (2012) New developments in the ATSAS program package for small-angle scattering data analysis. J Appl Crystallogr 45:342–350

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Porod G (1951) Die Röntgenkleinwinkelstreuung von dichtgepackten kolloiden Systemen: I. Teil. Kolloid-Z 124:83–114

    Article  CAS  Google Scholar 

  • Rambo RP, Tainer JA (2013) Accurate assessment of mass, models and resolution by small-angle scattering. Nature 496:477–481

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schneidman-Duhovny D, Hammel M, Sali A (2010) FoXS: a web server for rapid computation and fitting of SAXS profiles. Nucleic Acids Res 38:W540–W544

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Svergun DI (1992) Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J Appl Crystallogr 25:495–503

    Article  CAS  Google Scholar 

  • Svergun D, Barberato C, Koch MHJ (1995) CRYSOL – a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates. J Appl Crystallogr 28:768–773

    Article  CAS  Google Scholar 

  • Svergun DI (1999) Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys J 76:2879–2886

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Svergun DI, Petoukhov MV, Koch MHJ (2001) Determination of domain structure of proteins from X-ray solution scattering. Biophys J 80:2946–2953

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Svergun DI, Koch MHJ, Timmins PA, May RP (2013) Small angle x-ray and neutron scattering from solutions of biological macromolecules. Oxford University Press

    Google Scholar 

  • Trewhella J et al (2017) 2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr Sect Struct Biol 73:710–728

    Article  CAS  Google Scholar 

  • Tuukkanen AT, Kleywegt GJ, Svergun DI (2016) Resolution of ab initio shapes determined from small-angle scattering. IUCrJ 3:440–447

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Valentini E, Kikhney AG, Previtali G, Jeffries CM, Svergun DI (2015) SASBDB, a repository for biological small-angle scattering data. Nucleic Acids Res 43:D357–D363

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Central European Institute of Technology, Masaryk University, Brno, Czech Republic

    Tomáš Klumpler

Authors
  1. Tomáš Klumpler
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tomáš Klumpler .

Editor information

Editors and Affiliations

  1. CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic

    Jan Hejátko

  2. Structural Biology Laboratory, Nara Institute of Science and Technology, Ikoma, Nara, Japan

    Toshio Hakoshima

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG, part of Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Klumpler, T. (2018). Biological Small-Angle X-Ray Scattering (SAXS). In: Hejátko, J., Hakoshima, T. (eds) Plant Structural Biology: Hormonal Regulations. Springer, Cham. https://doi.org/10.1007/978-3-319-91352-0_14

Download citation

Publish with us

Policies and ethics

Search

Navigation