Abstract
In natural conditions, the oocyte and embryo are subjected to ever-changing dynamic processes. However, the routine-assisted reproductive technologies today involve the use of static in vitro culture systems. The objective was to determine whether there is any difference in the viability of embryos after in vitro culture under static and mechanical micro-vibration conditions. The viability of embryonic cells (9624 embryos) generated from 4436 couples after in vitro culture was evaluated. For groups ≤29 years, 30–34 years, 35–39 years, and ≥40 years, the following rates of high-quality embryos without fragmentation (2–4 blastomeres on Day 2; 6–8 blastomeres and compacting morula on Day 3; blastocyst, expanded and hatching blastocyst on Day 5) were detected (static vs. vibration, respectively): 65% vs. 71%, 44% vs. 69%, 67% vs. 76% (for statistic significant differences between respective rates in these three groups P < 0.05), and 67% vs. 66% (P > 0.1). The following baby-take-home rates were determined for groups ≤29 years, 30–34 years, 35–39 years, and ≥ 40 years (static vs. vibration, respectively): 30% vs. 31% (P > 0.1, increasing only on the level of tendency), 28% vs. 37%, 23% vs. 29%, and 9% vs. 15% (differences between respective rates in these three groups with P < 0.05). It was concluded that in vitro culture of embryos under micro-vibration (with a mimic of conditions in nature whereby oviductal fluid is mechanically agitated by the epithelial cilia) significantly increases the baby-take-home rate for patients 30 years of age and older.
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Is the routine procedure of in vitro culture of oocytes/embryos mimicking conditions in nature?
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What are the limitations of in vitro development of oocytes/embryos?
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What is the source of micro-vibration in the testing equipment?
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Is using of micro-vibration beneficial by in vitro culture of oocytes and embryos?
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Isachenko, V., Sterzik, K., Rahimi, G., Mallmann, P., Isachenko, E. (2019). In Vitro Culture of Oocytes and Embryos with Micro-vibration: “Naturalization” of Routine IVF Procedure. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) In Vitro Fertilization. Springer, Cham. https://doi.org/10.1007/978-3-319-43011-9_19
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DOI: https://doi.org/10.1007/978-3-319-43011-9_19
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