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Cerebrolysin Attenuates Blood–Brain Barrier and Brain Pathology Following Whole Body Hyperthermia in the Rat

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Brain Edema XIV

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 106))

Abstract

The possibility that Cerebrolysin, a mixture of several neurotrophic factors, has some neuroprotective effects on whole body hyperthermia (WBH) induced breakdown of the blood–brain barrier (BBB), blood–CSF barrier (BCSFB), brain edema formation and neuropathology were examined in a rat model. Rats subjected to a 4 h heat stress at 38°C in a biological oxygen demand (BOD) incubator exhibited profound increases in BBB and BCSFB permeability to Evans blue and radioiodine tracers compared to controls. Hippocampus, caudate nucleus, thalamus and hypothalamus exhibited pronounced increase in water content and brain pathology following 4 h heat stress. Pretreatment with Cerebrolysin (1, 2 or 5 mL/kg i.v.) 24 h before WBH significantly attenuated breakdown of the BBB or BCSFB and brain edema formation. This effect was dose dependent. Interestingly, the cell and tissue injury following WBH in cerebrolysin-treated groups were also considerably reduced. These novel observations suggest that cerebrolysin can attenuate WBH induced BBB and BCSFB damage resulting in neuroprotection.

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Acknowledgements

This study is supported by Grants from Swedish Medical Research Council, Stockholm (HSS 02710); National Institute of Health (R01 AG 027910, CEJ), USA; Alexander von Humboldt Foundation (HSS), Germany; Astra Zeneca Mölndal, Sweden (HSS); Society for the study on Neuroplasticity and Neuroprotection (SSNN), Romania and Ebewe Pharma, Austria (HSS). Technical assistance of Kärstin Flink, Ingmarie Olsson, Margareta Butler, Inga Hörte, Mari-Anne Carlsson and Ulla Nilsson as well as the secretarial assistance of Angela Ludwig and Nancy Johanson are highly appreciated.

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Authors and Affiliations

  1. Laboratory of Carebrovascular Research, Department of Surgical Science, Anesthesiology and Intensive Care Medicine, University Hospital, Frödingsgatan 12:28, Uppsala, SE-75421, Sweden

    Hari Shanker Sharma & Aruna Sharma

  2. Neurochemistry Laboratory, Division of Neurotoxicology National Centre for Toxicological Research/FDA, Jefferson, AR, USA

    Hari Shanker Sharma & Aruna Sharma

  3. EBEWE Neuro-Pharma, Mondseestrasse 11, Unterach, A-4866, Austria

    Sibilla Zimmermann-Meinzingen

  4. Department of Clinical Neurosciences, Brown Medical School, Rhode Island Hospital, Providence, RI, 02903, USA

    Conrad E. Johanson

Authors
  1. Hari Shanker Sharma
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  2. Sibilla Zimmermann-Meinzingen
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  3. Aruna Sharma
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  4. Conrad E. Johanson
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Corresponding author

Correspondence to Hari Shanker Sharma .

Editor information

Editors and Affiliations

  1. Medical Research Center, PAN Warszawa, Ceglowska St. 80, Warszawa, 01-809, Poland

    Zbigniew Czernicki

  2. Klinikum Großhadern, Universitätsklinikum München, Marchioninistr. 15, München, 81377, Germany

    Alexander Baethmann

  3. Dept. Neurosurgery, Musashino Red Cross Hospital, Kyonan-cho 1-26-1, Musashino City, Tokyo, 180, Japan

    Umeo Ito

  4. School of Medicine, Nihon University, Oyaguchikami-machi 30-1 , Tokyo, 173-8610, Japan

    Yoichi Katayama

  5. Dept. Neuropathology, Medical Research Institute, Yushima 1-5-45, Tokyo, 113-8510, Japan

    Toshihiko Kuroiwa

  6. Dept. Neurosurgery, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne, NE46BE, United Kingdom

    David Mendelow

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© 2010 Springer-Verlag/Wien

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Sharma, H.S., Zimmermann-Meinzingen, S., Sharma, A., Johanson, C.E. (2010). Cerebrolysin Attenuates Blood–Brain Barrier and Brain Pathology Following Whole Body Hyperthermia in the Rat. In: Czernicki, Z., Baethmann, A., Ito, U., Katayama, Y., Kuroiwa, T., Mendelow, D. (eds) Brain Edema XIV. Acta Neurochirurgica Supplementum, vol 106. Springer, Vienna. https://doi.org/10.1007/978-3-211-98811-4_60

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  • DOI: https://doi.org/10.1007/978-3-211-98811-4_60

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  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-98758-2

  • Online ISBN: 978-3-211-98811-4

  • eBook Packages: MedicineMedicine (R0)

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